AUTEN-67 (Synonyms: Autophagy enhancer-67)

Cat. No.: HY-117924 Purity: ≥92.0%: FAQs
Data Sheet SDS COA Handling Instructions

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AUTEN-67 (Autophagy enhancer-67) is an orally active autophagy enhancer and MTMR14 inhibitor. AUTEN-67 has anti-aging and neuroprotective effects. AUTEN-67 protects neurons from stress-induced cell death. AUTEN-67 also restores nesting behavior in a mice model of Alzheimer disease.

For research use only. We do not sell to patients.

AUTEN-67 Chemical Structure

CAS No. : 1783800-77-0

Size	Price	Stock	Quantity
1 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Other Forms of AUTEN-67:

AUTEN-67 inner salt Get quote

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Description

In Vitro

AUTEN-67 (2-100 µM, 3 h) inhibits MTMR14 by nearly 3%-70%, induces autophagic flux, and promotes the survival in HeLa cells^[1].
AUTEN-67 (10-100 µM, 2 h) induces autophagy in Drosophila via inhibiting EDTP^[1].
AUTEN-67 (1-50 µM) decreases levels of LC3B-II, and protects neurons from oxidative stress (increases cell viability) in murine primary neurons^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

AUTEN-67 enhances autophagy in zebrafish (10, 50 µM)and mice (e 50 µmol/g body weight, i.p.)^[1].
AUTEN-67 (19 mg/kg, p.o., 3 times a week, for 3 month) restores nesting behavior and decreases APP level in the Alzheimer disease mice model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Alzheimer disease model (Mice expressing the human APP)^[1]
Dosage:	19 mg/kg, 3 times a week for 3 month
Administration:	Oral administration
Result:	Restored nesting behavior by around 30%. Decreased Amyloid β levels in the hemibrain of mice.

Molecular Weight

474.45

Formula

C₂₃H₁₄N₄O₆S

CAS No.

1783800-77-0

Appearance

Solid

Color

Orange to red

SMILES

O=S(C1=CC=C([N+]([O-])=O)C=C1)(NC(C2=O)=C(N3C4=CC=CC=C4N=C3)C(C5=C2C=CC=C5)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 12.5 mg/mL (26.35 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1077 mL	10.5385 mL	21.0770 mL
5 mM	0.4215 mL	2.1077 mL	4.2154 mL
10 mM	0.2108 mL	1.0539 mL	2.1077 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.63 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥92.0%

Select Batch:

Data Sheet (279 KB) SDS (252 KB)

COA (258 KB)

Handling Instructions (2659 KB)

References

[1]. Papp D, et al. AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects. Autophagy. 2016;12(2):273-86. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1077 mL	10.5385 mL	21.0770 mL	52.6926 mL
	5 mM	0.4215 mL	2.1077 mL	4.2154 mL	10.5385 mL
	10 mM	0.2108 mL	1.0539 mL	2.1077 mL	5.2693 mL
	15 mM	0.1405 mL	0.7026 mL	1.4051 mL	3.5128 mL
	20 mM	0.1054 mL	0.5269 mL	1.0539 mL	2.6346 mL
	25 mM	0.0843 mL	0.4215 mL	0.8431 mL	2.1077 mL

AUTEN-67 Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AUTEN-671783800-77-0Autophagy enhancer-67AUTEN67AUTEN 67Autophagy enhancer67Autophagy enhancer 67AutophagyAlzheimer diseaseHeLaDrosophilaneuronoxidative stressAPPInhibitorinhibitorinhibit

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