2. Apoptosis Autophagy Cell Cycle/DNA Damage PI3K/Akt/mTOR
  3. Apoptosis Autophagy DNA/RNA Synthesis Akt mTOR
  4. Autophagy inducer 7

Autophagy inducer 7 (Compound SSA) is an Autophagy and Apoptosis inducer. Autophagy inducer 7 activates autophagy by inhibiting Akt/mTOR signaling and the expression of downstream proteins. Autophagy inducer 7 suppresses DNA synthesis and causes a G0-G1 cell-cycle arrest. Autophagy inducer 7 inhibits tumor cell growth.

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Autophagy inducer 7 Chemical Structure

Autophagy inducer 7 Chemical Structure

CAS No. : 944159-20-0

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Based on 1 publication(s) in Google Scholar

Autophagy inducer 7 Related Antibodies

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Description

Autophagy inducer 7 (Compound SSA) is an Autophagy and Apoptosis inducer. Autophagy inducer 7 activates autophagy by inhibiting Akt/mTOR signaling and the expression of downstream proteins. Autophagy inducer 7 suppresses DNA synthesis and causes a G0-G1 cell-cycle arrest. Autophagy inducer 7 inhibits tumor cell growth[1].

In Vitro

Autophagy inducer 7 (72 h) inhibits the growth of human lung adenocarcinoma cell lines, with IC50s of 1.95 μM (HOP-62), 4.72 μM (A549), 3.05μM (H1299)[1].
Autophagy inducer 7 (0-10 μM, 24 h) induces apoptosis in HOP-62, A549, H1299 cells, but not significant as Sulindac sulfide (HY-B1786) and Staurosporine (HY-15141)[1].
Autophagy inducer 7 (0-7.5 μM, 24 h) inhibits DNA synthesis in HOP-62, A549, H1299 cells, and increases the percentage of cells in the G0-G1 phase of the cell cycle[1].
Autophagy inducer 7 (0-7.5 μM, 24 h) induces autophagy and increases autophagic flux by inhibiting Akt/mTOR/p70S6k signaling in A549 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Autophagy inducer 7 Related Antibodies

Apoptosis Analysis[1]

Cell Line: HOP-62, A549, H1299 cells
Concentration: 2.5, 5, 7.5, 10 μM
Incubation Time: 24 h
Result: Induced caspase3/7 activation dose-dependently.
Induced cleaved PARP expression at 10 μM.
Increased Annexin-V surface staining and PI labeling.

Cell Autophagy Assay[1]

Cell Line: HOP-62, A549, H1299 cells
Concentration: 2.5, 5, 7.5, 10 μM
Incubation Time: 24 h
Result: Induced dose- and time-dependent cleavage of cytosolic LC3-I to autophagosome-associated LC3-II.
Decreased p62 expression.
Displayed a striking accumulation of vesicle-like structures within the cytoplasm.
Increased both the number and size of acidic vesicles within the cytoplasm.
Molecular Weight

410.55

Formula

C24H27FN2OS
CAS No.
Appearance

Solid

SMILES

CN(C)CCNC(CC1=C(C)/C(C2=CC=C(F)C=C21)=C/C3=CC=C(C=C3)SC)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (121.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4358 mL 12.1788 mL 24.3576 mL
5 mM 0.4872 mL 2.4358 mL 4.8715 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4358 mL 12.1788 mL 24.3576 mL 60.8939 mL
5 mM 0.4872 mL 2.4358 mL 4.8715 mL 12.1788 mL
10 mM 0.2436 mL 1.2179 mL 2.4358 mL 6.0894 mL
15 mM 0.1624 mL 0.8119 mL 1.6238 mL 4.0596 mL
20 mM 0.1218 mL 0.6089 mL 1.2179 mL 3.0447 mL
25 mM 0.0974 mL 0.4872 mL 0.9743 mL 2.4358 mL
30 mM 0.0812 mL 0.4060 mL 0.8119 mL 2.0298 mL
40 mM 0.0609 mL 0.3045 mL 0.6089 mL 1.5223 mL
50 mM 0.0487 mL 0.2436 mL 0.4872 mL 1.2179 mL
60 mM 0.0406 mL 0.2030 mL 0.4060 mL 1.0149 mL
80 mM 0.0304 mL 0.1522 mL 0.3045 mL 0.7612 mL
100 mM 0.0244 mL 0.1218 mL 0.2436 mL 0.6089 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Autophagy inducer 7944159-20-0Autophagy inducer7Autophagy inducer-7ApoptosisAutophagyDNA/RNA SynthesisAktmTORPKBProtein kinase BMammalian target of Rapamycinlung adenocarcinomaHOP-62A549H1299cell-cycle arrestInhibitorinhibitorinhibit

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