BCI (Synonyms: (E)-BCI)

Name: BCI
Brand: MedChemExpress
SKU: HY-115502
Rating: 5.0 (11 reviews)

Cat. No.: HY-115502 Purity: 99.73%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

BCI ((E)-BCI) is a DUSP6 (dual specificity phosphatase 6) inhibitor. BCI shows anti-inflammatory activity and decreases reactive oxygen species (ROS) production. BCI can be used in inflammatory disease research.

For research use only. We do not sell to patients.

BCI Chemical Structure

CAS No. : 1245792-51-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 116	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 116	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 165	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 275	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 11 publication(s) in Google Scholar

Other Forms of BCI:

(E/Z)-BCI Get quote
BCI hydrochloride Get quote

10 Publications Citing Use of MCE BCI

: BCI purchased from MedChemExpress. Usage Cited in: Kaohsiung J Med Sci. 2022 Dec 16. [Abstract]; BCI (8 μM) reverses FAM3D-induced inhibition of gluconeogenesis and expression of DUSP1 in HepG2 cells.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	IC₅₀	11.5 μM Compound: 1, BCI	Inhibition of Myc-tagged human Dusp1 expressed in TPA-induced human HeLa cells assessed as decrease in dephosphorylation of pERK measured by immunostaining Inhibition of Myc-tagged human Dusp1 expressed in TPA-induced human HeLa cells assessed as decrease in dephosphorylation of pERK measured by immunostaining	[PMID: 19578332]
HeLa	IC₅₀	12.3 μM Compound: 1, BCI	Inhibition of Myc-tagged human Dusp6 expressed in TPA-induced human HeLa cells assessed as decrease in dephosphorylation of pERK measured by immunostaining Inhibition of Myc-tagged human Dusp6 expressed in TPA-induced human HeLa cells assessed as decrease in dephosphorylation of pERK measured by immunostaining	[PMID: 19578332]

In Vitro

BCI (100 ng/mL; 24 h) downregulats the expression of DUSP6 in RAW264.7 macrophage cells^[2].
? BCI (0-1 nM; 24 h) inhibits the expression of IL-1β and IL-6 in lipopolysaccharide- (LPS-) activated macrophages^[2].
? BCI (0-4 nM; 24 h) decreases ROS production and activates the Nrf2 Pathway in LPS-activated macrophages^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	RAW264.7 macrophage cells
Concentration:	100 ng/mL
Incubation Time:	24 hours
Result:	Showed DUSP6 protein downregulation.

RT-PCR^[2]

Cell Line:	RAW264.7 macrophage cells
Concentration:	0-1 nM
Incubation Time:	24 hours
Result:	Inhibited the expression of IL-1β and IL-6 mRNA in LPS-activated macrophages.

Molecular Weight

317.43

Formula

C₂₂H₂₃NO

CAS No.

1245792-51-1

Appearance

Solid

Color

Off-white to yellow

SMILES

O=C1/C(C(NC2CCCCC2)C3=C1C=CC=C3)=C/C4=CC=CC=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (393.79 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1503 mL	15.7515 mL	31.5030 mL
5 mM	0.6301 mL	3.1503 mL	6.3006 mL
10 mM	0.3150 mL	1.5752 mL	3.1503 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (6.55 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.55 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.73%

Select Batch:

Data Sheet (282 KB) SDS (393 KB)

COA (258 KB) LCMS (103 KB)

Handling Instructions (2659 KB)

References

[1]. Korotchenko VN, et al. In vivo structure-activity relationship studies support allosteric targeting of a dual specificity phosphatase. Chembiochem. 2014 Jul 7;15(10):1436-45. [Content Brief]

[2]. Zhang F, et al. DUSP6 Inhibitor (E/Z)-BCI Hydrochloride Attenuates Lipopolysaccharide-Induced Inflammatory Responses in Murine Macrophage Cells via Activating the Nrf2 Signaling Axis and Inhibiting the NF-κB Pathway. Inflammation. 2019 Apr;42(2):672-681. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1503 mL	15.7515 mL	31.5030 mL	78.7575 mL
	5 mM	0.6301 mL	3.1503 mL	6.3006 mL	15.7515 mL
	10 mM	0.3150 mL	1.5752 mL	3.1503 mL	7.8758 mL
	15 mM	0.2100 mL	1.0501 mL	2.1002 mL	5.2505 mL
	20 mM	0.1575 mL	0.7876 mL	1.5752 mL	3.9379 mL
	25 mM	0.1260 mL	0.6301 mL	1.2601 mL	3.1503 mL
	30 mM	0.1050 mL	0.5251 mL	1.0501 mL	2.6253 mL
	40 mM	0.0788 mL	0.3938 mL	0.7876 mL	1.9689 mL
	50 mM	0.0630 mL	0.3150 mL	0.6301 mL	1.5752 mL
	60 mM	0.0525 mL	0.2625 mL	0.5251 mL	1.3126 mL
	80 mM	0.0394 mL	0.1969 mL	0.3938 mL	0.9845 mL
	100 mM	0.0315 mL	0.1575 mL	0.3150 mL	0.7876 mL

BCI Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BCI1245792-51-1(E)-BCIPhosphataseanti-inflammatory activityRAW264.7 macrophage cellInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

BCI (Synonyms: (E)-BCI)

Customer Review

Other Forms of BCI:

BCI Related Antibodies

10 Publications Citing Use of MCE BCI

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity

Purity & Documentation

References

Customer Review

BCI Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

BCI Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report