BCI hydrochloride (Synonyms: (E)-BCI hydrochloride)

Name: BCI hydrochloride
Brand: MedChemExpress
SKU: HY-115502A
Rating: 5.0 (9 reviews)

Cat. No.: HY-115502A Purity: 99.93%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

BCI ((E)-BCI) hydrochloride is a DUSP6 (dual specificity phosphatase 6) inhibitor. BCI hydrochloride shows anti-inflammatory activity and decreases reactive oxygen species (ROS) production. BCI hydrochloride can be used in inflammatory disease research.

For research use only. We do not sell to patients.

BCI hydrochloride Chemical Structure

CAS No. : 95130-23-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 109	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 109	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 140	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 440	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 700	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1120	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of BCI hydrochloride:

(E/Z)-BCI Get quote
BCI Get quote

8 Publications Citing Use of MCE BCI hydrochloride

: BCI hydrochloride purchased from MedChemExpress. Usage Cited in: Kaohsiung J Med Sci. 2022 Dec 16. [Abstract]; BCI (8 μM) reverses FAM3D-induced inhibition of gluconeogenesis and expression of DUSP1 in HepG2 cells.

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Biological Activity
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Description

IC₅₀ & Target

DUSP6^[1]

In Vitro

BCI (100 ng/mL; 24 h) downregulats the expression of DUSP6 in RAW264.7 macrophage cells^[2].
BCI (0-1 nM; 24 h) inhibits the expression of IL-1β and IL-6 in lipopolysaccharide- (LPS-) activated macrophages^[2].
BCI (0-4 nM; 24 h) decreases ROS production and activates the Nrf2 Pathway in LPS-activated macrophages^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:	RAW264.7 macrophage cells
Concentration:	100 ng/mL
Incubation Time:	24 hours
Result:	Showed DUSP6 protein downregulation.

RT-PCR^[2]

Cell Line:	RAW264.7 macrophage cells
Concentration:	0-1 nM
Incubation Time:	24 hours
Result:	Inhibited the expression of IL-1β and IL-6 mRNA in LPS-activated macrophages.

Molecular Weight

353.89

Formula

C₂₂H₂₄ClNO

CAS No.

95130-23-7

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1/C(C(NC2CCCCC2)C3=C1C=CC=C3)=C/C4=CC=CC=C4.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 15.62 mg/mL (44.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8257 mL	14.1287 mL	28.2574 mL
5 mM	0.5651 mL	2.8257 mL	5.6515 mL
10 mM	0.2826 mL	1.4129 mL	2.8257 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.56 mg/mL (4.41 mM); Clear solution

This protocol yields a clear solution of ≥ 1.56 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.6 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.56 mg/mL (4.41 mM); Clear solution

This protocol yields a clear solution of ≥ 1.56 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.6 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1.56 mg/mL (4.41 mM); Clear solution

This protocol yields a clear solution of ≥ 1.56 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (15.6 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (280 KB) SDS (252 KB)

COA (256 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Korotchenko VN, et al. In vivo structure-activity relationship studies support allosteric targeting of a dual specificity phosphatase. Chembiochem. 2014 Jul 7;15(10):1436-45. [Content Brief]

[2]. Zhang F, et al. DUSP6 Inhibitor (E/Z)-BCI Hydrochloride Attenuates Lipopolysaccharide-Induced Inflammatory Responses in Murine Macrophage Cells via Activating the Nrf2 Signaling Axis and Inhibiting the NF-κB Pathway. Inflammation. 2019 Apr;42(2):672-681. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.8257 mL	14.1287 mL	28.2574 mL	70.6434 mL
	5 mM	0.5651 mL	2.8257 mL	5.6515 mL	14.1287 mL
	10 mM	0.2826 mL	1.4129 mL	2.8257 mL	7.0643 mL
	15 mM	0.1884 mL	0.9419 mL	1.8838 mL	4.7096 mL
	20 mM	0.1413 mL	0.7064 mL	1.4129 mL	3.5322 mL
	25 mM	0.1130 mL	0.5651 mL	1.1303 mL	2.8257 mL
	30 mM	0.0942 mL	0.4710 mL	0.9419 mL	2.3548 mL
	40 mM	0.0706 mL	0.3532 mL	0.7064 mL	1.7661 mL

BCI hydrochloride Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BCI95130-23-7(E)-BCIPhosphataseanti-inflammatory activityRAW264.7 macrophage cellInhibitorinhibitorinhibit

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BCI hydrochloride (Synonyms: (E)-BCI hydrochloride)

Customer Review

Other Forms of BCI hydrochloride:

BCI hydrochloride Related Antibodies

8 Publications Citing Use of MCE BCI hydrochloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

Purity & Documentation

References

Customer Review

BCI hydrochloride Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

BCI hydrochloride Related Classifications

Keywords:

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Customer Review

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