trans-Benzylideneacetone (Synonyms: trans-Benzalacetone)

Name: trans-Benzylideneacetone
Brand: MedChemExpress
SKU: HY-W012595A
Rating: 4.5 (1 reviews)

Cat. No.: HY-W012595A Purity: 99.29%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

trans-Benzylideneacetone (trans-Benzalacetone), a metabolite of gram-negative entomopathogenic bacterium Xenorhabdus nematophila, is an enzyme inhibitor against phospholipase A2 (PLA2). trans-Benzylideneacetone is an immunosuppressant.

For research use only. We do not sell to patients.

trans-Benzylideneacetone Chemical Structure

CAS No. : 1896-62-4

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10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

Other Forms of trans-Benzylideneacetone:

Benzylideneacetone Get quote

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Phospholipase A PLD Phospholipase C

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

PLA2

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	2.8 μg/mL Compound: 9	Cytotoxicity against human A549 cells after 2 days by sulforhodamine B assay Cytotoxicity against human A549 cells after 2 days by sulforhodamine B assay	[PMID: 17067159]
HeLa	IC₅₀	> 20 μM Compound: Butenone	Antiproliferative activity against human HeLa cells after 72 hrs by trypan blue assay Antiproliferative activity against human HeLa cells after 72 hrs by trypan blue assay	[PMID: 19155103]
HL-60	IC₅₀	> 20 μM Compound: Butenone	Antiproliferative activity against human HL60 cells after 72 hrs by trypan blue assay Antiproliferative activity against human HL60 cells after 72 hrs by trypan blue assay	[PMID: 19155103]
KB	IC₅₀	5.3 μg/mL Compound: 9	Cytotoxicity against human KB cells after 2 days by sulforhodamine B assay Cytotoxicity against human KB cells after 2 days by sulforhodamine B assay	[PMID: 17067159]
KB	IC₅₀	5.6 μg/mL Compound: 9	Cytotoxicity against multidrug-resistant human KB-VCR cells after 2 days by sulforhodamine B assay Cytotoxicity against multidrug-resistant human KB-VCR cells after 2 days by sulforhodamine B assay	[PMID: 17067159]

In Vitro

Benzylideneacetone (trans-Benzylideneacetone; 0.005, 0.05, 0.5, 5, 50, 500, 5000 μM) inhibits hemocytespreading behavior of hemocytes of S. exigua larvae. Benzylideneacetone enhances virulence of Bacillus thuringiensisagainst beet armyworm^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

146.19

Formula

C₁₀H₁₀O

CAS No.

1896-62-4

Appearance

<39°C Solid,>42°C Liquid

Color

Off-white to light yellow

SMILES

CC(/C=C/C1=CC=CC=C1)=O.[E]

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Microorganisms

a metabolite of gram-negative entomopathogenic bacterium Xenorhabdus nematophila

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (684.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.8404 mL	34.2021 mL	68.4041 mL
5 mM	1.3681 mL	6.8404 mL	13.6808 mL
10 mM	0.6840 mL	3.4202 mL	6.8404 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (17.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (17.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (17.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.29%

Select Batch:

Data Sheet (271 KB) SDS (393 KB)

COA (247 KB)

Handling Instructions (2659 KB)

References

[1]. Kwon B, et al. Benzylideneacetone, an immunosuppressant, enhances virulence of Bacillus thuringiensisagainst beet armyworm (Lepidoptera: Noctuidae). J Econ Entomol. 2008 Feb;101(1):36-41. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.8404 mL	34.2021 mL	68.4041 mL	171.0103 mL
	5 mM	1.3681 mL	6.8404 mL	13.6808 mL	34.2021 mL
	10 mM	0.6840 mL	3.4202 mL	6.8404 mL	17.1010 mL
	15 mM	0.4560 mL	2.2801 mL	4.5603 mL	11.4007 mL
	20 mM	0.3420 mL	1.7101 mL	3.4202 mL	8.5505 mL
	25 mM	0.2736 mL	1.3681 mL	2.7362 mL	6.8404 mL
	30 mM	0.2280 mL	1.1401 mL	2.2801 mL	5.7003 mL
	40 mM	0.1710 mL	0.8551 mL	1.7101 mL	4.2753 mL
	50 mM	0.1368 mL	0.6840 mL	1.3681 mL	3.4202 mL
	60 mM	0.1140 mL	0.5700 mL	1.1401 mL	2.8502 mL
	80 mM	0.0855 mL	0.4275 mL	0.8551 mL	2.1376 mL
	100 mM	0.0684 mL	0.3420 mL	0.6840 mL	1.7101 mL

trans-Benzylideneacetone Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

trans-Benzylideneacetone1896-62-4trans-BenzalacetonePhospholipasegram-negativeentomopathogenicbacteriumXenorhabdusnematophilaimmunosuppressantInhibitorinhibitorinhibit

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trans-Benzylideneacetone (Synonyms: trans-Benzalacetone)

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Other Forms of trans-Benzylideneacetone:

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•Related Screening Libraries:

•Related Small Molecules:

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Biological Activity

Purity & Documentation

References

Customer Review

trans-Benzylideneacetone Related Antibodies

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Complete Stock Solution Preparation Table

trans-Benzylideneacetone Related Classifications

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