Berberrubine chloride

Cat. No.: HY-125850 Purity: 99.28%: FAQs
Data Sheet SDS COA Handling Instructions

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Berberrubine chloride is an active metabolite of berberine, attenuates ulcerative colitis in mice model.

For research use only. We do not sell to patients.

Berberrubine chloride Chemical Structure

CAS No. : 15401-69-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Berberrubine chloride:

Berberrubine chloride (Standard) Get quote

1 Publications Citing Use of MCE Berberrubine chloride

•Molecules. 2024 May 14.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Berberrubine chloride is an active metabolite of berberine, attenuates ulcerative colitis in mice model^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
Bel-7402	IC₅₀	34.31 μM Compound: 2, Berberrubine	Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 48 hrs by MTT assay	10.1007/s00044-013-0796-9
BGC-823	IC₅₀	7.66 μM Compound: 2, Berberrubine	Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay Cytotoxicity against human BGC823 cells after 48 hrs by MTT assay	10.1007/s00044-013-0796-9
CCRF-CEM	CC₅₀	169.2 μM Compound: 5	Cytotoxicity against human CEM cells expressing green fluorescent protein after 48 hrs by MTT assay Cytotoxicity against human CEM cells expressing green fluorescent protein after 48 hrs by MTT assay	[PMID: 21295891]
CCRF-CEM	EC₅₀	2.8 μM Compound: 5	Antiviral activity against 0.05 MOI Human immunodeficiency virus 1 NL4.3 infected in human CEM cells expressing green fluorescent protein assessed as p24 antigen production measured 7 days post infection by ELISA Antiviral activity against 0.05 MOI Human immunodeficiency virus 1 NL4.3 infected in human CEM cells expressing green fluorescent protein assessed as p24 antigen production measured 7 days post infection by ELISA	[PMID: 21295891]
HepG2	IC₅₀	> 50 μM Compound: 2, Berberrubine	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 23182088]
HepG2	IC₅₀	> 50 μM Compound: 2, Berberrubine	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 23182088]
HL-60	IC₅₀	11.36 μM Compound: 2, Berberrubine	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	10.1007/s00044-013-0796-9
HT-29	IC₅₀	> 50 μM Compound: 2, Berberrubine	Cytotoxicity against human HT-29 cells after 24 hrs by MTT assay Cytotoxicity against human HT-29 cells after 24 hrs by MTT assay	[PMID: 23182088]
HT-29	IC₅₀	36.93 μM Compound: 2, Berberrubine	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay	[PMID: 23182088]
KB	IC₅₀	6.92 μM Compound: 2, Berberrubine	Cytotoxicity against human KB cells after 48 hrs by MTT assay Cytotoxicity against human KB cells after 48 hrs by MTT assay	10.1007/s00044-013-0796-9

Molecular Weight

357.79

Formula

C₁₉H₁₆ClNO₄

CAS No.

15401-69-1

Appearance

Solid

Color

Yellow to reddish brown

SMILES

COC1=C(O)C2=C[N+]3=C(C(C(CC3)=C4)=CC5=C4OCO5)C=C2C=C1.[Cl-]

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (13.97 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : 2 mg/mL (5.59 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7949 mL	13.9747 mL	27.9494 mL
5 mM	0.5590 mL	2.7949 mL	5.5899 mL
10 mM	0.2795 mL	1.3975 mL	2.7949 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water
Solubility: 5 mg/mL (13.97 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.28%

Select Batch:

Data Sheet (274 KB) SDS (392 KB)

COA (252 KB) HNMR (253 KB) RP-HPLC (263 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Yu XT, et al. Berberrubine attenuates mucosal lesions and inflammation in dextran sodium sulfate-induced colitis in mice. PLoS One. 2018 Mar 14;13(3):e0194069. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	2.7949 mL	13.9747 mL	27.9494 mL	69.8734 mL
Ethanol / DMSO	5 mM	0.5590 mL	2.7949 mL	5.5899 mL	13.9747 mL
DMSO	10 mM	0.2795 mL	1.3975 mL	2.7949 mL	6.9873 mL