β-Amyrin

Name: β-Amyrin
Brand: MedChemExpress
SKU: HY-N2922
Rating: 4.5 (1 reviews)

Cat. No.: HY-N2922 Purity: 99.95%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

β-Amyrin shows effectively counteract amyloid β (Aβ)-induced impairment of long-term potentiation (LTP). β-Amyrin is a promising candidate for Alzheimer's disease (AD) research. β-Amyrin exhibits anti-inflammatory effects, protective activity against pulmonary fibrosis, and notable antibacterial capabilities. β-Amyrin is an orally active natural triterpenoid compound.

For research use only. We do not sell to patients.

β-Amyrin Chemical Structure

CAS No. : 559-70-6

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
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50 mg	Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of β-Amyrin:

β-Amyrin (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	23.55 μM Compound: 10	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 28482219]
B16	ED₅₀	> 50 μM Compound: 14	Induction of melanogenesis in mouse B16 2F2 cells assessed as intracellular melanin content after 3 days Induction of melanogenesis in mouse B16 2F2 cells assessed as intracellular melanin content after 3 days	[PMID: 12027734]
B16	IC₅₀	48.8 μM Compound: 14	Growth inhibition of mouse B16 2F2 cells after 3 days Growth inhibition of mouse B16 2F2 cells after 3 days	[PMID: 12027734]
DU-145	IC₅₀	27.88 μM Compound: 10	Cytotoxicity against human DU145 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human DU145 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 28482219]
HEK293	IC₅₀	94.4 μM Compound: 10	Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HEK293 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 28482219]
HeLa	IC₅₀	> 50 μM Compound: 4	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 19447618]
HeLa	IC₅₀	27.94 μM Compound: 10	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 28482219]
J774	IC₅₀	> 234.3 μM Compound: 2	Cytotoxicity against mouse J774 cells by alamar blue assay Cytotoxicity against mouse J774 cells by alamar blue assay	[PMID: 17637068]
MCF7	IC₅₀	> 50 μM Compound: 4	Cytotoxicity against human MCF7 cells by MTT assay Cytotoxicity against human MCF7 cells by MTT assay	[PMID: 19447618]
MCF7	IC₅₀	35.31 μM Compound: 10	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 48 hrs by MTT assay	[PMID: 28482219]
SK-MEL-1	IC₅₀	> 50 μM Compound: 4	Cytotoxicity against human SK-MEL-1 cells by MTT assay Cytotoxicity against human SK-MEL-1 cells by MTT assay	[PMID: 19447618]

In Vitro

β-Amyrin (1-100 μM, 0-80 min) blocks Aβ-induced synaptic dysfunction in the hippocampal tissues of mice^[1].
β-Amyrin (1-10 μg, 24 h 15 min) shows antioxidant activity in cultured hPBMCs (human peripheral blood mononuclear cells)^[2].
β-Amyrin (5-20 μg/mL, 16-24 h) significantly inhibits PGE2 (HY-101952), IL-6 secretion and NF-κB activation dose-dependently in LPS-induced hPBMCs^[2].
β-Amyrin (5 μM, 2 h) exhibits antibacterial mode of action, leading promoting apoptosis-like death in E. coli by producing ROS.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[4]

Cell Line:	E. coli (BW 25113)
Concentration:	5 μM
Incubation Time:	2 h
Result:	Decreased GSH/GSSG ratio and induced membrane depolarization and DNA cleavage. Activated caspase-like protein expression.

In Vivo

β-Amyrin (4 mg/kg, p.o., daily, 5 days) ameliorates object recognition memory deficit and neurogenesis impairments in the hippocampus of Aβ-induced AD model mice^[1].
β-Amyrin (20-100 μg, i.p.) inhibits carrageenan-induced paw edema of rats^[2].
β-Amyrin (20-80 mg/kg, i.g., daily, 5 days) has a potent efficacy in protecting against BLM-induced mice pulmonary fibrosis via suppressing inflammatory response and oxidative stress^[3].
Pharmacokinetic parameters of β-Amyrin in rats

	β-Amyrin standard IV (group 3)	β-Amyrin standard oral dose (group 2)
Dose (mg kg−1)	1	3	300 (equivalent of 3mg kg−1 β-amyrin)
V (ml kg−1)	1317
AUC_{0→24 h} (min μg ml−1)	411	10.6	47.3
Cl (ml min−1 kg−1)	2.04
t_1/2λZ (h)	10.2	10.3	8.3
MRT_{0→24 h} (h) (h)	8	8.5	8.7
C_max (ng ml−1) (h) (h)		18.7	75
t_max (h)	-	3 (n = 2) or 5 (n = 1)	3 (n = 3) or 5 (n = 1)
F (%)		0.86	3.83

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male CD-1 mice, 26-28 g, 6 weeks old. 5 μL of Aβ was acutely injected into the left lateral ventricle by hand^[1].
Dosage:	4 mg/kg
Administration:	p.o., daily, 5 days
Result:	Increased number of transitions between the light and dark compartments. Increased the total exploration time on the light compartment of the apparatus and the time spent in open arms. Ameliorated the reduction of neurogenesis.

Animal Model:	Adult male rats of Sprague–Dawley strain, weighing 150-180 g. Carrageenan induces edema in the hind paw of the rat^[2].
Dosage:	20, 50, 100 μg/kg
Administration:	i.p.
Result:	Significant decreased edema by in a dose dependent manner.

Animal Model:	Adult male rats of Sprague–Dawley strain, weighing 150-180 g. Carrageenan induces Use Bleomycin (BLM) (HY-108345), 5mg/kg, one-time intratracheally injection, in 50μL saline per mouse to induce the marine model of pulmonary fibrosis^[3].
Dosage:	20, 40, 80 mg/kg
Administration:	i.g., daily, 5 days
Result:	Promoted the values of SO2 and PO2 and inhibited the value of PCO2. Significantly improved inflammatory cell infiltration, the thickened alveolar septum and the lesion collapses of the alveoli and formation of flaky lung tissue.

Molecular Weight

426.72

Formula

C₃₀H₅₀O

CAS No.

559-70-6

Appearance

Solid

Color

White to off-white

SMILES

CC1(C)[C@@H](O)CC[C@]2(C)[C@@]3([H])CC=C4[C@]5([H])CC(C)(C)CC[C@@](C)5CC[C@](C)4[C@@](C)3CC[C@@]12[H]

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

Ethanol : 5 mg/mL (11.72 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3435 mL	11.7173 mL	23.4346 mL
5 mM	0.4687 mL	2.3435 mL	4.6869 mL
10 mM	0.2343 mL	1.1717 mL	2.3435 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.5 mg/mL (1.17 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 0.5 mg/mL (1.17 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.95%

Select Batch:

Data Sheet (285 KB) SDS (393 KB)

COA (265 KB) HNMR (256 KB) RP-HPLC (248 KB)

Handling Instructions (2659 KB)

References

[1]. Park HJ, et al. β-Amyrin Ameliorates Alzheimer's Disease-Like Aberrant Synaptic Plasticity in the Mouse Hippocampus. Biomol Ther (Seoul). 2019 Jul 30. [Content Brief]

[2]. Krishnan K, et al. Anti-inflammatory potential of β-amyrin, a triterpenoid isolated from Costus igneus. Inflammopharmacology. 2014 Dec;22(6):373-85. [Content Brief]

[3]. Cai Z, Liu J, et al. β-Amyrin ameliorates pulmonary fibrosis by inhibiting inflammatory response and oxidative stress in mice. Pak J Pharm Sci. 2023 Mar;36(2):431-436. [Content Brief]

[4]. Han G, et al. Antibacterial Mode of Action of β-Amyrin Promotes Apoptosis-Like Death in Escherichia coli by Producing Reactive Oxygen Species. J Microbiol Biotechnol. 2022 Dec 28;32(12):1547-1552. [Content Brief]

[5]. Ching J, et al. Quantification of α- and β-amyrin in rat plasma by gas chromatography-mass spectrometry: application to preclinical pharmacokinetic study. J Mass Spectrom. 2011 May;46(5):457-64. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	2.3435 mL	11.7173 mL	23.4346 mL	58.5864 mL
	5 mM	0.4687 mL	2.3435 mL	4.6869 mL	11.7173 mL
	10 mM	0.2343 mL	1.1717 mL	2.3435 mL	5.8586 mL

β-Amyrin Related Classifications

Neurological Disease Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

β-Amyrin559-70-6Amyloid-βNF-κBβ-amyloid peptideAβAbetaNuclear factor-κBNuclear factor-kappaBAlzheimer’s diseaseSynaptic plasticityanti-inflammatorypulmonary fibrosisantibacterialInhibitorinhibitorinhibit

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β-Amyrin

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β-Amyrin Related Antibodies

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Complete Stock Solution Preparation Table

β-Amyrin Related Classifications

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