Bisindolylmaleimide IV (Synonyms: Arcyriarubin A)

Name: Bisindolylmaleimide IV
Brand: MedChemExpress
SKU: HY-108254
Rating: 4.5 (1 reviews)

Cat. No.: HY-108254 Purity: 99.62%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Bisindolylmaleimide IV (Arcyriarubin A) is a potent protein kinase C (PKC) inhibitor, with IC₅₀s ranging from 0.1 to 0.55 μM. Bisindolylmaleimide IV also inhibits PKA (IC₅₀=3.1-11.8μM). Bisindolylmaleimide IV is a potent, selective inhibitor of human cytomegalovirus (HCMV) replication in cell culture with an IC₅₀ of 0.2 μM.

For research use only. We do not sell to patients.

Bisindolylmaleimide IV Chemical Structure

CAS No. : 119139-23-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 77	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 77	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 112	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 226	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 315	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 440	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PKC Isoform Specific Products:

View All Isoforms

PKC PKCα PKCβ PKCγ PKCδ PKCε PKCη PKCζ PKCθ PKCμ PKC-ι ℩ PKCι

Biological Activity
Purity & Documentation
References
Customer Review

Description

Bisindolylmaleimide IV (Arcyriarubin A) is a potent protein kinase C (PKC) inhibitor, with IC₅₀s ranging from 0.1 to 0.55 μM. Bisindolylmaleimide IV also inhibits PKA (IC₅₀=3.1-11.8μM)^[1]. Bisindolylmaleimide IV is a potent, selective inhibitor of human cytomegalovirus (HCMV) replication in cell culture with an IC₅₀ of 0.2 μM^[2].

IC₅₀ & Target^[1]^[2]

PKC

0.1-0.55 μM (IC₅₀)

HCMV

0.2 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	4.14 μM Compound: Bisindolyl-maleimide	Cytotoxicity activity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity activity against human A549 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 30119011]
A549	IC₅₀	56.61 μM Compound: Ro-316233	Antiproliferative activity against human A549 cells after 48 hrs by MTT assay Antiproliferative activity against human A549 cells after 48 hrs by MTT assay	[PMID: 19447039]
ECa-109 cell line	IC₅₀	15.9 μM Compound: Bisindolyl-maleimide	Cytotoxicity activity against human Eca-109 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity activity against human Eca-109 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 30119011]
ECa-109 cell line	IC₅₀	83.55 μM Compound: Ro-316233	Antiproliferative activity against human ECA109 cells after 48 hrs by MTT assay Antiproliferative activity against human ECA109 cells after 48 hrs by MTT assay	[PMID: 19447039]
K562	IC₅₀	17.46 μM Compound: Ro-316233	Antiproliferative activity against human K562 cells after 48 hrs by MTT assay Antiproliferative activity against human K562 cells after 48 hrs by MTT assay	[PMID: 19447039]
KB	IC₅₀	53.78 μM Compound: Ro-316233	Antiproliferative activity against human KB cells after 48 hrs by MTT assay Antiproliferative activity against human KB cells after 48 hrs by MTT assay	[PMID: 19447039]
PC-3	IC₅₀	10.3 μM Compound: Bisindolyl-maleimide	Cytotoxicity activity against human PC-3 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity activity against human PC-3 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 30119011]
SMMC-7721	IC₅₀	> 100 μM Compound: Ro-316233	Antiproliferative activity against human SMMC7721 cells after 48 hrs by MTT assay Antiproliferative activity against human SMMC7721 cells after 48 hrs by MTT assay	[PMID: 19447039]
SMMC-7721	IC₅₀	13.2 μM Compound: Bisindolyl-maleimide	Cytotoxicity activity against human SMMC-7721 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity activity against human SMMC-7721 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 30119011]

In Vitro

Bisindolylmaleimide IV (Arcyriarubin A) inhibits PKC with an IC₅₀ of 0.1 μM in 1 % DMSO, while the IC₅₀ of 0.55 μM in 10 % DMSO^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

327.34

Formula

C₂₀H₁₃N₃O₂

CAS No.

119139-23-0

Appearance

Solid

Color

Orange to red

SMILES

O=C(C(C1=CNC2=C1C=CC=C2)=C3C4=CNC5=C4C=CC=C5)NC3=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (381.87 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0549 mL	15.2746 mL	30.5493 mL
5 mM	0.6110 mL	3.0549 mL	6.1099 mL
10 mM	0.3055 mL	1.5275 mL	3.0549 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.62%

Select Batch:

Data Sheet (278 KB) SDS (252 KB)

COA (256 KB) HNMR (251 KB) LCMS (255 KB)

Handling Instructions (2659 KB)

References

[1]. Davis PD, et al. Inhibitors of protein kinase C. 1. 2,3-Bisarylmaleimides. J Med Chem. 1992 Jan;35(1):177-84. [Content Brief]

[2]. Slater MJ, et al. Indolocarbazoles: potent, selective inhibitors of human cytomegalovirus replication. Bioorg Med Chem. 1999 Jun;7(6):1067-74. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.0549 mL	15.2746 mL	30.5493 mL	76.3732 mL
	5 mM	0.6110 mL	3.0549 mL	6.1099 mL	15.2746 mL
	10 mM	0.3055 mL	1.5275 mL	3.0549 mL	7.6373 mL
	15 mM	0.2037 mL	1.0183 mL	2.0366 mL	5.0915 mL
	20 mM	0.1527 mL	0.7637 mL	1.5275 mL	3.8187 mL
	25 mM	0.1222 mL	0.6110 mL	1.2220 mL	3.0549 mL
	30 mM	0.1018 mL	0.5092 mL	1.0183 mL	2.5458 mL
	40 mM	0.0764 mL	0.3819 mL	0.7637 mL	1.9093 mL
	50 mM	0.0611 mL	0.3055 mL	0.6110 mL	1.5275 mL
	60 mM	0.0509 mL	0.2546 mL	0.5092 mL	1.2729 mL
	80 mM	0.0382 mL	0.1909 mL	0.3819 mL	0.9547 mL
	100 mM	0.0305 mL	0.1527 mL	0.3055 mL	0.7637 mL

Bisindolylmaleimide IV Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Bisindolylmaleimide IV119139-23-0Arcyriarubin APKCCMVProtein kinase CCytomegalovirushumancytomegalovirusdiseaseselectiveDMSOdiscriminativeInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Email Address *

Phone Number

Department *

Organization Name *

Country or Region *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Bisindolylmaleimide IV (Synonyms: Arcyriarubin A)

Customer Review

Bisindolylmaleimide IV Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PKC Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Bisindolylmaleimide IV Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Bisindolylmaleimide IV Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report