2. Metabolic Enzyme/Protease
  3. Liposome
  4. C14-4

C14-4 is an ionizable lipid utilized for the synthesis of lipid nanoparticles (LNPs). C14-4 enhances mRNA delivery, enabling the effective transport of mRNA to primary human T cells, which in turn induces functional protein expression. C14-4 demonstrates high transfection efficiency while maintaining low cytotoxicity.

For research use only. We do not sell to patients.

C14-4 Chemical Structure

C14-4 Chemical Structure

CAS No. : 2639634-80-1

Size Price Stock Quantity
1 mg USD 85 In-stock
5 mg USD 250 In-stock
10 mg USD 390 In-stock
25 mg USD 750 In-stock
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Based on 1 publication(s) in Google Scholar

C14-4 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

C14-4 is an ionizable lipid utilized for the synthesis of lipid nanoparticles (LNPs). C14-4 enhances mRNA delivery, enabling the effective transport of mRNA to primary human T cells, which in turn induces functional protein expression. C14-4 demonstrates high transfection efficiency while maintaining low cytotoxicity[1].
In Vitro

C14-4 (30 ng C14-4 LNPs/60000 cells, 48 h) promotes the expression of luciferase in primary human T cell Jurkat cells by enhancing mRNA delivery with low cytotoxicity[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

C14-4 Related Antibodies

Cell Cytotoxicity Assay[1].

Cell Line: Jurkat cells
Concentration: 30 ng C14-4 LNPs/60000 cells
Incubation Time: 48 h
Result: Promoted the expression of luciferase and had minimal cytotoxicity.
Molecular Weight

1365.30

Formula

C84H173N5O7
CAS No.
Appearance

Liquid (Density: 0.946±0.06 g/cm3)

Color

Colorless to light yellow

SMILES

OC(CCCCCCCCCCCC)CN(CCN1CCN(CC1)CCOCCN(CC(CCCCCCCCCCCC)O)CC(CCCCCCCCCCCC)O)CCOCCN(CC(CCCCCCCCCCCC)O)CC(CCCCCCCCCCCC)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : 100 mg/mL (73.24 mM; Need ultrasonic)

DMSO : 100 mg/mL (73.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7324 mL 3.6622 mL 7.3244 mL
5 mM 0.1465 mL 0.7324 mL 1.4649 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (1.83 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.01%

SDS (252 KB)

COA (244 KB) HNMR (333 KB) RP-HPLC (220 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 0.7324 mL 3.6622 mL 7.3244 mL 18.3110 mL
5 mM 0.1465 mL 0.7324 mL 1.4649 mL 3.6622 mL
10 mM 0.0732 mL 0.3662 mL 0.7324 mL 1.8311 mL
15 mM 0.0488 mL 0.2441 mL 0.4883 mL 1.2207 mL
20 mM 0.0366 mL 0.1831 mL 0.3662 mL 0.9155 mL
25 mM 0.0293 mL 0.1465 mL 0.2930 mL 0.7324 mL
30 mM 0.0244 mL 0.1221 mL 0.2441 mL 0.6104 mL
40 mM 0.0183 mL 0.0916 mL 0.1831 mL 0.4578 mL
50 mM 0.0146 mL 0.0732 mL 0.1465 mL 0.3662 mL
60 mM 0.0122 mL 0.0610 mL 0.1221 mL 0.3052 mL
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C14-4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

C14-42639634-80-1LiposomeIonizable lipidmRNA deliverytransfectionInhibitorinhibitorinhibit

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