2. Immunology/Inflammation Apoptosis MAPK/ERK Pathway Anti-infection NF-κB Metabolic Enzyme/Protease
  3. Toll-like Receptor (TLR) MyD88 Apoptosis Ferroptosis JNK Fungal Reactive Oxygen Species
  4. Chloranil

Chloranil  (Synonyms: Tetrachloro-p-benzoquinone; TCBQ)

Cat. No.: HY-Y0278 Purity: 98%
SDS COA Handling Instructions Technical Support

Chloranil (Tetrachloro-p-benzoquinone), an orally active metabolite of pentachlorophenol and hexachlorobenzene, is a widely used fungicide. Chloranil can induce ROS production. Chloranil induces neutrophil extracellular traps through the ROS-JNK-NOX2 pathway. Chloranil induces ferroptosis and neuroinflammation. Chloranil induces apoptosis of mouse embryonic stem cells .

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Chloranil Chemical Structure

Chloranil Chemical Structure

CAS No. : 118-75-2

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Other Forms of Chloranil:

Chloranil Related Antibodies

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Description

Chloranil (Tetrachloro-p-benzoquinone), an orally active metabolite of pentachlorophenol and hexachlorobenzene, is a widely used fungicide. Chloranil can induce ROS production. Chloranil induces neutrophil extracellular traps through the ROS-JNK-NOX2 pathway. Chloranil induces ferroptosis and neuroinflammation. Chloranil induces apoptosis of mouse embryonic stem cells[1][2][3][4][5] .

IC50 & Target[1]

TLR4

 

Cellular Effect
Cell Line Type Value Description References
MRC5 IC50
> 100 μM
Compound: 16
Cytotoxicity against human MRC5 cells assessed as cell growth inhibition after 48 hrs by alamar blue assay
Cytotoxicity against human MRC5 cells assessed as cell growth inhibition after 48 hrs by alamar blue assay
[PMID: 26638044]
In Vitro

Chloranil (20 μM; 12-24 h) induces ferroptosis in PC12 cells[1].
Chloranil (0-50 μM; 24 h) induces apoptosis in mouse embryonic stem cells in a dose-dependent manner[2].
Chloranil (10 μM; 1.5-3 h) induces neutrophil extracellular traps by ROS-JNK-NOX2 signaling pathway in mouse neutrophils[3].
Chloranil (25 μM; 6 h) decreases the viability of PC12 cells, increases the expression and interaction of TLR4 and MyD88, and up-regulates the expression and interaction of CD14 and MD2[4].
Chloranil (25 μM; 6 h) stimulates the expression of inflammatory factors and activates MAPK signaling pathway in PC12 cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chloranil Related Antibodies

Western Blot Analysis[3]

Cell Line: Mouse neutrophil
Concentration: 10 μM
Incubation Time: 1.5 h
Result: Significantly increased the phosphorylation level of JNK.
Increased the level of NOX2.

Western Blot Analysis[4]

Cell Line: PC12 cells
Concentration: 25 μM
Incubation Time: 6 h
Result: Enhanced the expression levels of TLR4 and MyD88.
Enhanced the expression levels of CD14 and MD2.
Enhanced the expression levels of TNF-a, IL-1β and IL-6.
Enhanced the expression levels of c-fos, c-jun and AP-1.
Increased the phosphorylation levels of p38, JNK and ERK.
In Vivo

Chloranil (10 mg/kg; Oral administration; 2 weeks) induces nerve damage in mice[4].
Chloranil (1 mg/kg; Intraperitoneal injection; 3 days) has a strong hepatotoxic effect in mice, but can be alleviated by chlorogenic acid (HY-N0055)[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6 TLR4 wild-type mice (5-8 weeks old)[4]
Dosage: 10 mg/kg
Administration: Oral administration (p.o.); 2 weeks
Result: Caused severe coagulation necrosis, nuclear pyknosis, anachromasis and shrunken neuronal bodies in both cortical and the hippocampus region.
Caused cortical damage.
Decreased NeuN-positive neurons and increased highly activated microglia in cortex.
Animal Model: Male Kunming mice (22 ± 2 g)[5]
Dosage: 1 mg/kg
Administration: Intraperitoneal injection (i.p.); 3 days
Result: Caused marked liver cell necrosis and inflammation but not apoptosis, and this damage was alleviated by Chlorogenic acid (HY-N0055).
Enhanced serum ALT, AST activities, TBIL content, hepatic oxidative stress and lipid peroxidation, decreased GSH content and inhibited the activities of antioxidant enzymes.
Up-regulated HO-1 and NQO1 expression.
Molecular Weight

245.88

Formula

C6Cl4O2
CAS No.
Appearance

Solid

Color

Light yellow to green yellow

SMILES

O=C1C(Cl)=C(Cl)C(C(Cl)=C1Cl)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (20.34 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0670 mL 20.3351 mL 40.6702 mL
5 mM 0.8134 mL 4.0670 mL 8.1340 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Working solution concentration: mg/mL
Purity & Documentation

Purity: 98%

SDS (759 KB)

COA (255 KB) CNMR (124 KB) MS (79 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.0670 mL 20.3351 mL 40.6702 mL 101.6756 mL
5 mM 0.8134 mL 4.0670 mL 8.1340 mL 20.3351 mL
10 mM 0.4067 mL 2.0335 mL 4.0670 mL 10.1676 mL
15 mM 0.2711 mL 1.3557 mL 2.7113 mL 6.7784 mL
20 mM 0.2034 mL 1.0168 mL 2.0335 mL 5.0838 mL
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Chloranil Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Chloranil118-75-2Tetrachloro-p-benzoquinone TCBQToll-like Receptor (TLR)MyD88ApoptosisFerroptosisJNKFungalReactive Oxygen Speciesc-Jun N-terminal kinaseMouse neutrophilPC12 cellsMouse embryonic stem cellsInhibitorinhibitorinhibit

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