2. Metabolic Enzyme/Protease NF-κB Immunology/Inflammation Anti-infection
  3. HIF/HIF Prolyl-Hydroxylase Reactive Oxygen Species Bacterial Influenza Virus Endogenous Metabolite
  4. Chlorogenic acid

Chlorogenic acid  (Synonyms: 3-O-Caffeoylquinic acid; Heriguard; NSC-407296)

Cat. No.: HY-N0055 Purity: 99.35%
COA Handling Instructions

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Chlorogenic acid is a major phenolic compound in Lonicera japonica Thunb. It is an orally active antioxidant activity, antibacterial, hepatoprotective, cardioprotective, anti-inflammatory, antipyretic, neuroprotective, anti-obesity, antiviral, anti-microbial, anti-hypertension compound.

For research use only. We do not sell to patients.

Chlorogenic acid Chemical Structure

Chlorogenic acid Chemical Structure

CAS No. : 327-97-9

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10 mM * 1 mL in DMSO
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Based on 15 publication(s) in Google Scholar

Other Forms of Chlorogenic acid:

Chlorogenic acid Related Antibodies

Top Publications Citing Use of Products

    Chlorogenic acid purchased from MedChemExpress. Usage Cited in: Int J Med Microbiol. 2023 Jan 24.

    Chlorogenic acid (CGA; 50 μg/mL; 1 h) inhibits the Klebsiella pneumoniae (Kp)-induced increase in p38 expression levels as well as increases in p-p38/p-38 and p-MK2/MK2 levels, in RLE6TN cells.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Chlorogenic acid is a major phenolic compound in Lonicera japonica Thunb. It is an orally active antioxidant activity, antibacterial, hepatoprotective, cardioprotective, anti-inflammatory, antipyretic, neuroprotective, anti-obesity, antiviral, anti-microbial, anti-hypertension compound[1][2][3].

    In Vitro

    Chlorogenic acid (10 μM, 16 h) decreases HIF-1α protein levels in CoCl2 induced hypoxic A549 cells, but does not affect HIF-1α mRNA level[1].
    Chlorogenic acid (10 μM, 24 h) inhibits the hypoxia-induced HUVEC cell migration, invasion and tube formation of vascular endothelial cells[1].
    Chlorogenic acid (25, 50 μM, 24 h) inhibits cell proliferation of Huh7 cells, and reduces the number of invading and migrating cells[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Chlorogenic acid Related Antibodies
    In Vivo

    Chlorogenic acid (10 μM, s.c.) inhibits VEGF (200 ng/mL)-induced angiogenesis in C57BL/6J mice, by suppression of AKT activation (Matrigel plug assay)[1].
    Chlorogenic acid (10-100 mg/kg, p.o.) shows protective effects against experimental reflux esophagitis in rats[3].
    Chlorogenic acid (10 mg/kg, i.v.) prevents endotoxic mortality and induced TNF-α release of LPS-intoxicated C57BL/6 mice, and ameliorates acute liver injury of LPS/GalN-challenged mice[2].
    Chlorogenic acid (ip, 25-200 mg/kg) inhibits tumor growth in NOD/SCID mice inoculated with Huh7 or H446 cells[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Experimental reflux esophagitis (RE) in rats[1]
    Dosage: 10, 30, 100 mg/kg
    Administration: p.o.
    Result: Reduced esophageal lipid peroxidation (marker: MDA) and increased the reduced glutathione/oxidized glutathione ratio.
    Inhibited the increases in the serum level of TNF-α, and expressions of iNOS and COX-2 protein.
    Animal Model: LPS/GalN-challenged mice[2]
    Dosage: 10 mg/kg
    Administration: i.v.
    Result: Increased survival rates of LPS/GalN-intoxicated mice.
    Inhibited LPS/GalN-induced phosphorylation of NF-κB p65 or c-Jun, without affecting p-IRF3 levels in the liver lobules.
    Clinical Trial
    Molecular Weight

    354.31

    Formula

    C16H18O9
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C([C@@]1(O)C[C@@H](OC(/C=C/C2=CC=C(O)C(O)=C2)=O)[C@H](O)[C@H](O)C1)O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (282.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : ≥ 20 mg/mL (56.45 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8224 mL 14.1119 mL 28.2239 mL
    5 mM 0.5645 mL 2.8224 mL 5.6448 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (7.06 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (7.06 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.55%

    COA (249 KB) LCMS (267 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 2.8224 mL 14.1119 mL 28.2239 mL 70.5597 mL
    5 mM 0.5645 mL 2.8224 mL 5.6448 mL 14.1119 mL
    10 mM 0.2822 mL 1.4112 mL 2.8224 mL 7.0560 mL
    15 mM 0.1882 mL 0.9408 mL 1.8816 mL 4.7040 mL
    20 mM 0.1411 mL 0.7056 mL 1.4112 mL 3.5280 mL
    25 mM 0.1129 mL 0.5645 mL 1.1290 mL 2.8224 mL
    30 mM 0.0941 mL 0.4704 mL 0.9408 mL 2.3520 mL
    40 mM 0.0706 mL 0.3528 mL 0.7056 mL 1.7640 mL
    50 mM 0.0564 mL 0.2822 mL 0.5645 mL 1.4112 mL
    DMSO 60 mM 0.0470 mL 0.2352 mL 0.4704 mL 1.1760 mL
    80 mM 0.0353 mL 0.1764 mL 0.3528 mL 0.8820 mL
    100 mM 0.0282 mL 0.1411 mL 0.2822 mL 0.7056 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Chlorogenic acid Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Chlorogenic acid327-97-93-O-Caffeoylquinic acid Heriguard NSC-407296NSC407296NSC 407296NSC-407296HIF/HIF Prolyl-HydroxylaseReactive Oxygen SpeciesBacterialInfluenza VirusEndogenous MetaboliteHypoxia-inducible factorsHIFsHIF-PHantioxidant activityantibacterialhepatoprotectivecardioprotectiveanti-inflammatoryantipyreticneuroprotectiveanti-obesityantiviralanti-microbialanti-hypertensionInhibitorinhibitorinhibit

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