Chlorogenic acid (Synonyms: 3-O-Caffeoylquinic acid; Heriguard; NSC-407296)

Name: Chlorogenic acid
Brand: MedChemExpress
SKU: HY-N0055
Rating: 5.0 (15 reviews)

Cat. No.: HY-N0055 Purity: 99.35%: FAQs
Data Sheet SDS COA Handling Instructions

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Chlorogenic acid is a major phenolic compound in Lonicera japonica Thunb. It is an orally active antioxidant activity, antibacterial, hepatoprotective, cardioprotective, anti-inflammatory, antipyretic, neuroprotective, anti-obesity, antiviral, anti-microbial, anti-hypertension compound.

For research use only. We do not sell to patients.

Chlorogenic acid Chemical Structure

CAS No. : 327-97-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 66	In-stock	Estimated Time of Arrival: December 31
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100 mg	USD 30	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
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Based on 15 publication(s) in Google Scholar

Other Forms of Chlorogenic acid:

14 Publications Citing Use of MCE Chlorogenic acid

: Chlorogenic acid purchased from MedChemExpress. Usage Cited in: Int J Med Microbiol. 2023 Jan 24.; Chlorogenic acid (CGA; 50 μg/mL; 1 h) inhibits the Klebsiella pneumoniae (Kp)-induced increase in p38 expression levels as well as increases in p-p38/p-38 and p-MK2/MK2 levels, in RLE6TN cells.

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HIF-1α HIF

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
BALB/3T3	IC₅₀	> 280 μM Compound: 14	Growth inhibition of BALB/c mouse cloned 3T3/A31 cells after 72 hrs by nigrosin assay Growth inhibition of BALB/c mouse cloned 3T3/A31 cells after 72 hrs by nigrosin assay	[PMID: 10096863]
BMDC	IC₅₀	> 50 μM Compound: 4	Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced IL-12 p40 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BMDC	IC₅₀	> 50 μM Compound: 4	Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced IL-6 production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BMDC	IC₅₀	> 50 μM Compound: 4	Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA Inhibition of LPS-induced TNF-alpha production in wild-type C57BL/6 mouse BMDC pretreated with compound for 1 hr before LPS treatment measured 16 hrs by ELISA	[PMID: 25769817]
BMDM	IC₅₀	86 μM Compound: Chlorogenic acid	Inhibition of M-CSF/RANKL-induced osteoclast differentiation in C57BL/6 mouse bone marrow macrophage assessed as reduction in multinucleated TRAP+ cells incubated for 6 days with fresh media replacement on day 3 and measured on day 6 by TRAP staining-base Inhibition of M-CSF/RANKL-induced osteoclast differentiation in C57BL/6 mouse bone marrow macrophage assessed as reduction in multinucleated TRAP+ cells incubated for 6 days with fresh media replacement on day 3 and measured on day 6 by TRAP staining-base	[PMID: 31257875]
HCT-116	IC₅₀	367 μM Compound: chlorogenic acid	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 12880319]
HeLa	IC₅₀	> 200 μM Compound: Chlorogenic acid	Inhibition of HDAC in human HeLa cells using Boc-Lys(AC)-AMC as substrate after 24 to 48 hrs by spectrofluorometry Inhibition of HDAC in human HeLa cells using Boc-Lys(AC)-AMC as substrate after 24 to 48 hrs by spectrofluorometry	[PMID: 26996372]
HeLa	IC₅₀	375 μM Compound: 26	Inhibition of HDAC in human Hela cells nuclear extracts by fluorimetric assay Inhibition of HDAC in human Hela cells nuclear extracts by fluorimetric assay	[PMID: 19520580]
SW480	IC₅₀	353 μM Compound: chlorogenic acid	Cytotoxicity against human SW480 cells after 72 hrs by MTT assay Cytotoxicity against human SW480 cells after 72 hrs by MTT assay	[PMID: 12880319]

In Vitro

Chlorogenic acid (10 μM, 16 h) decreases HIF-1α protein levels in CoCl₂ induced hypoxic A549 cells, but does not affect HIF-1α mRNA level^[1].
Chlorogenic acid (10 μM, 24 h) inhibits the hypoxia-induced HUVEC cell migration, invasion and tube formation of vascular endothelial cells^[1].
Chlorogenic acid (25, 50 μM, 24 h) inhibits cell proliferation of Huh7 cells, and reduces the number of invading and migrating cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Chlorogenic acid (10 μM, s.c.) inhibits VEGF (200 ng/mL)-induced angiogenesis in C57BL/6J mice, by suppression of AKT activation (Matrigel plug assay)^[1].
Chlorogenic acid (10-100 mg/kg, p.o.) shows protective effects against experimental reflux esophagitis in rats^[3].
Chlorogenic acid (10 mg/kg, i.v.) prevents endotoxic mortality and induced TNF-α release of LPS-intoxicated C57BL/6 mice, and ameliorates acute liver injury of LPS/GalN-challenged mice^[2].
Chlorogenic acid (ip, 25-200 mg/kg) inhibits tumor growth in NOD/SCID mice inoculated with Huh7 or H446 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Experimental reflux esophagitis (RE) in rats^[1]
Dosage:	10, 30, 100 mg/kg
Administration:	p.o.
Result:	Reduced esophageal lipid peroxidation (marker: MDA) and increased the reduced glutathione/oxidized glutathione ratio. Inhibited the increases in the serum level of TNF-α, and expressions of iNOS and COX-2 protein.

Animal Model:	LPS/GalN-challenged mice^[2]
Dosage:	10 mg/kg
Administration:	i.v.
Result:	Increased survival rates of LPS/GalN-intoxicated mice. Inhibited LPS/GalN-induced phosphorylation of NF-κB p65 or c-Jun, without affecting p-IRF3 levels in the liver lobules.

Clinical Trial

Molecular Weight

354.31

Formula

C₁₆H₁₈O₉

CAS No.

327-97-9

Appearance

Solid

Color

White to off-white

SMILES

O=C([C@@]1(O)C[C@@H](OC(/C=C/C2=CC=C(O)C(O)=C2)=O)[C@H](O)[C@H](O)C1)O

Structure Classification

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (282.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 20 mg/mL (56.45 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.8224 mL	14.1119 mL	28.2239 mL
5 mM	0.5645 mL	2.8224 mL	5.6448 mL
10 mM	0.2822 mL	1.4112 mL	2.8224 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.06 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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mg/kg

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.55%

Select Batch:

Data Sheet (279 KB) SDS (393 KB)

COA (249 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. Park JJ, et al. Chlorogenic acid inhibits hypoxia-induced angiogenesis via down-regulation of the HIF-1α/AKT pathway. Cell Oncol (Dordr). 2015 Jan 6. [Content Brief]

[2]. Park SH, et al. IRAK4 as a Molecular Target in the Amelioration of Innate Immunity-Related Endotoxic Shock and Acute Liver Injury by Chlorogenic Acid. J Immunol. 2015 Feb 1;194(3):1122-30. [Content Brief]

[3]. Kang JW, et al. Protective Effects of Chlorogenic Acid against Experimental Reflux Esophagitis in Rats. Biomol Ther (Seoul). 2014 Sep;22(5):420-5. [Content Brief]

[4]. Huang S, et al. Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation. Theranostics. 2019 Sep 19;9(23):6745-6763. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.8224 mL	14.1119 mL	28.2239 mL	70.5597 mL
	5 mM	0.5645 mL	2.8224 mL	5.6448 mL	14.1119 mL
	10 mM	0.2822 mL	1.4112 mL	2.8224 mL	7.0560 mL
	15 mM	0.1882 mL	0.9408 mL	1.8816 mL	4.7040 mL
	20 mM	0.1411 mL	0.7056 mL	1.4112 mL	3.5280 mL
	25 mM	0.1129 mL	0.5645 mL	1.1290 mL	2.8224 mL
	30 mM	0.0941 mL	0.4704 mL	0.9408 mL	2.3520 mL
	40 mM	0.0706 mL	0.3528 mL	0.7056 mL	1.7640 mL
	50 mM	0.0564 mL	0.2822 mL	0.5645 mL	1.4112 mL
DMSO	60 mM	0.0470 mL	0.2352 mL	0.4704 mL	1.1760 mL
	80 mM	0.0353 mL	0.1764 mL	0.3528 mL	0.8820 mL
	100 mM	0.0282 mL	0.1411 mL	0.2822 mL	0.7056 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Chlorogenic acid Related Classifications

Help & FAQs

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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Chlorogenic acid (Synonyms: 3-O-Caffeoylquinic acid; Heriguard; NSC-407296)

Customer Review

Other Forms of Chlorogenic acid:

14 Publications Citing Use of MCE Chlorogenic acid

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•Related Screening Libraries:

•Related Small Molecules:

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View All HIF/HIF Prolyl-Hydroxylase Isoform Specific Products:

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Purity & Documentation

References

Customer Review

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Complete Stock Solution Preparation Table

Chlorogenic acid Related Classifications

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Chlorogenic acid (Synonyms: 3-O-Caffeoylquinic acid; Heriguard; NSC-407296)

Customer Review

Other Forms of Chlorogenic acid:

Chlorogenic acid Related Antibodies

14 Publications Citing Use of MCE Chlorogenic acid

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All HIF/HIF Prolyl-Hydroxylase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Chlorogenic acid Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Chlorogenic acid Related Classifications

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