CIL62

Name: CIL62
Brand: MedChemExpress
SKU: HY-136509
Rating: 4.5 (1 reviews)

Cat. No.: HY-136509 Purity: ≥97.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

CIL62 is a caspase-3/7-independent cell death inducer. The mechanism of action of CIL62 is Necrostatin-1 dependent cell death.

For research use only. We do not sell to patients.

CIL62 Chemical Structure

CAS No. : 117593-36-9

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 264	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 264	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 360	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1250	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 2000	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

CIL62 is a caspase-3/7-independent cell death inducer. The mechanism of action of CIL62 is Necrostatin-1 dependent cell death^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
COLO 205	IC₅₀	28 μM Compound: 3f	Antiproliferative activity against human COLO205 cells after 24 hrs by MTT assay Antiproliferative activity against human COLO205 cells after 24 hrs by MTT assay	[PMID: 22365759]
IMR-32	IC₅₀	41 μM Compound: 3f	Antiproliferative activity against human IMR32 cells after 24 hrs by MTT assay Antiproliferative activity against human IMR32 cells after 24 hrs by MTT assay	[PMID: 22365759]
K562	IC₅₀	36 μM Compound: 3f	Antiproliferative activity against human K562 cells after 24 hrs by MTT assay Antiproliferative activity against human K562 cells after 24 hrs by MTT assay	[PMID: 22365759]

In Vitro

19 µM (5 µg/mL) Necrostatin-1 suppresses CIL62, but CIL62 is not suppressed by the ferroptosis inducers (antioxidants or iron-chelators)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

382.45

Formula

C₂₃H₂₆O₅

CAS No.

117593-36-9

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(C(C2=CC=C(O)C=C2O)C3=C(CC(C)(C)CC3=O)O4)=C4CC(C)(C)C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (326.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6147 mL	13.0736 mL	26.1472 mL
5 mM	0.5229 mL	2.6147 mL	5.2294 mL
10 mM	0.2615 mL	1.3074 mL	2.6147 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (5.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥97.0%

Select Batch:

Data Sheet (276 KB) SDS (392 KB)

COA (254 KB) HNMR (284 KB)

Handling Instructions (2659 KB)

References

[1]. Kenichi Shimada, et al. Global Survey of Cell Death Mechanisms Reveals Metabolic Regulation of Ferroptosis. Nat Chem Biol. 2016 Jul;12(7):497-503. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6147 mL	13.0736 mL	26.1472 mL	65.3680 mL
	5 mM	0.5229 mL	2.6147 mL	5.2294 mL	13.0736 mL
	10 mM	0.2615 mL	1.3074 mL	2.6147 mL	6.5368 mL
	15 mM	0.1743 mL	0.8716 mL	1.7431 mL	4.3579 mL
	20 mM	0.1307 mL	0.6537 mL	1.3074 mL	3.2684 mL
	25 mM	0.1046 mL	0.5229 mL	1.0459 mL	2.6147 mL
	30 mM	0.0872 mL	0.4358 mL	0.8716 mL	2.1789 mL
	40 mM	0.0654 mL	0.3268 mL	0.6537 mL	1.6342 mL
	50 mM	0.0523 mL	0.2615 mL	0.5229 mL	1.3074 mL
	60 mM	0.0436 mL	0.2179 mL	0.4358 mL	1.0895 mL
	80 mM	0.0327 mL	0.1634 mL	0.3268 mL	0.8171 mL
	100 mM	0.0261 mL	0.1307 mL	0.2615 mL	0.6537 mL

CIL62 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CIL62117593-36-9CIL 62CIL-62OthersCaspase-3/7-independentnon-apoptoticNecrostatin-1elldeathInhibitorinhibitorinhibit

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CIL62

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CIL62 Related Antibodies

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Complete Stock Solution Preparation Table

CIL62 Related Classifications

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