Cjoc42

Name: Cjoc42
Brand: MedChemExpress
SKU: HY-138054
Rating: 4.5 (1 reviews)

Cat. No.: HY-138054 Purity: ≥99.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Cjoc42 is a compound capable of binding to gankyrin. Cjoc42 inhibits gankyrin activity in a dose-dependent manner. Cjoc42 prevents the decrease in p53 protein levels normally associated with high amounts of gankyrin. Cjoc42 restores p53-dependent transcription and sensitivity to DNA damage.

For research use only. We do not sell to patients.

Cjoc42 Chemical Structure

CAS No. : 2171519-89-2

Size	Price	Stock	Quantity
5 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 435	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 780	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1170	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1640	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 100 μM Compound: 1; cjoc42	Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 32738965]
A549	IC₅₀	> 100 μM Compound: 1: cjoc42	Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 35758870]
A549	IC₅₀	> 100 μM Compound: Cjoc42	Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 33865970]
Hep 3B2	IC₅₀	> 100 μM Compound: Cjoc42	Cytotoxicity against human Hep3B cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human Hep3B cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 33865970]
Hep 3B2	IC₅₀	> 50 μM Compound: cjoc42	Antiproliferative activity against human Hep3B cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human Hep3B cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31902711]
HepG2	IC₅₀	> 100 μM Compound: Cjoc42	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 33865970]
HepG2	IC₅₀	> 100 μM Compound: 1; cjoc42	Cytotoxicity against human Hep3B cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human Hep3B cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32738965]
HepG2	IC₅₀	> 100 μM Compound: 1; cjoc42	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 32738965]
HepG2	IC₅₀	> 50 μM Compound: cjoc42	Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31902711]
MDA-MB-231	IC₅₀	> 100 μM Compound: 1; cjoc42	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 32738965]
MDA-MB-231	IC₅₀	> 100 μM Compound: 1: cjoc42	Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 35758870]
MDA-MB-231	IC₅₀	> 100 μM Compound: Cjoc42	Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 33865970]

In Vitro

cjoc42 (0.5, 1, and 5 μM; 48 hours) dose-dependently restores p53 levels (comparable to mock transfected cells) and inhibits gankyrin-induced lowering of p53 levels in cells^[1].
cjoc42 (0.1, 1, and 10 μM; 24 hours) alone shows no dose-dependent change in cell viability^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	U2OS
Concentration:	0.5, 1, and 5 μM
Incubation Time:	48 hours
Result:	Dose-dependent restoration of p53 levels (comparable to mock transfected cells) and inhibited gankyrin-induced lowering of p53 levels in cells.

Molecular Weight

415.46

Formula

C₂₀H₂₁N₃O₅S

CAS No.

2171519-89-2

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(OC)C1=CC=C(N2N=NC(CCCOS(=O)(C3=CC=C(C)C=C3)=O)=C2)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (240.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4070 mL	12.0349 mL	24.0697 mL
5 mM	0.4814 mL	2.4070 mL	4.8139 mL
10 mM	0.2407 mL	1.2035 mL	2.4070 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.02 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (270 KB) SDS (251 KB)

COA (245 KB) HNMR (111 KB) LCMS (178 KB)

Handling Instructions (2659 KB)

References

[1]. Chattopadhyay A, et al. Discovery of a small-molecule binder of the oncoprotein gankyrin that modulates gankyrin activity in the cell. Sci Rep. 2016;6:23732. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4070 mL	12.0349 mL	24.0697 mL	60.1743 mL
	5 mM	0.4814 mL	2.4070 mL	4.8139 mL	12.0349 mL
	10 mM	0.2407 mL	1.2035 mL	2.4070 mL	6.0174 mL
	15 mM	0.1605 mL	0.8023 mL	1.6046 mL	4.0116 mL
	20 mM	0.1203 mL	0.6017 mL	1.2035 mL	3.0087 mL
	25 mM	0.0963 mL	0.4814 mL	0.9628 mL	2.4070 mL
	30 mM	0.0802 mL	0.4012 mL	0.8023 mL	2.0058 mL
	40 mM	0.0602 mL	0.3009 mL	0.6017 mL	1.5044 mL
	50 mM	0.0481 mL	0.2407 mL	0.4814 mL	1.2035 mL
	60 mM	0.0401 mL	0.2006 mL	0.4012 mL	1.0029 mL
	80 mM	0.0301 mL	0.1504 mL	0.3009 mL	0.7522 mL
	100 mM	0.0241 mL	0.1203 mL	0.2407 mL	0.6017 mL

Cjoc42 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cjoc422171519-89-2Cjoc 42Cjoc-42MDM-2/p53gankyrinp53transcriptionDNA damageInhibitorinhibitorinhibit

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Cjoc42

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Complete Stock Solution Preparation Table

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