2. Immunology/Inflammation
  3. Toll-like Receptor (TLR)
  4. CL264

CL264 is a TLR7-specific agonist for innate immune signals research.

For research use only. We do not sell to patients.

CL264 Chemical Structure

CL264 Chemical Structure

CAS No. : 1510712-69-2

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 352 In-stock
Solution
10 mM * 1 mL in DMSO USD 352 In-stock
Solid
5 mg USD 320 In-stock
10 mg USD 450 In-stock
25 mg USD 750 In-stock
50 mg USD 950 In-stock
100 mg USD 1350 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

CL264 Related Antibodies

Top Publications Citing Use of Products

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TLR3 TLR8 TLR9 TLR2 TLR4 TLR7 TLR1 TLR6

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

CL264 is a TLR7-specific agonist for innate immune signals research[1].

IC50 & Target[1]

TLR7

 

In Vitro

Toll like receptors (TLRs) are the best-studied class of receptors that are essential players in the detection of a range of lipid-, protein-, nucleic acid-based PAMPs. TLR7 is expressed predominantly in plasmacytoid dendritic cells (pDCs), macrophages and B cells. Many TLR7 modulators are interferon inducers, anti-cancer agents, anti-infectious agents, therapeutic agents for immunological diseases and vaccine adjuvants[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CL264 Related Antibodies
Molecular Weight

413.43

Formula

C19H23N7O4
CAS No.
Appearance

Solid

Color

Brown to gray

SMILES

O=C(O)CNC(C1=CC=C(CN2C(NC3=C(N)N=C(NCCCC)N=C23)=O)C=C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (60.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4188 mL 12.0939 mL 24.1879 mL
5 mM 0.4838 mL 2.4188 mL 4.8376 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.08 mg/mL (5.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.63%

COA (247 KB) HNMR (294 KB) LCMS (99 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4188 mL 12.0939 mL 24.1879 mL 60.4697 mL
5 mM 0.4838 mL 2.4188 mL 4.8376 mL 12.0939 mL
10 mM 0.2419 mL 1.2094 mL 2.4188 mL 6.0470 mL
15 mM 0.1613 mL 0.8063 mL 1.6125 mL 4.0313 mL
20 mM 0.1209 mL 0.6047 mL 1.2094 mL 3.0235 mL
25 mM 0.0968 mL 0.4838 mL 0.9675 mL 2.4188 mL
30 mM 0.0806 mL 0.4031 mL 0.8063 mL 2.0157 mL
40 mM 0.0605 mL 0.3023 mL 0.6047 mL 1.5117 mL
50 mM 0.0484 mL 0.2419 mL 0.4838 mL 1.2094 mL
60 mM 0.0403 mL 0.2016 mL 0.4031 mL 1.0078 mL
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CL264 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CL2641510712-69-2CL 264CL-264Toll-like Receptor (TLR)TLR7specificinnateimmuneInhibitorinhibitorinhibit

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