Coralyne chloride 

Coralyne chloride is a protoberberine alkaloid with potent anti-cancer activities. Coralyne chloride acts as a potent topoisomerase I poison and induces Top I mediated DNA cleavage^[2]. Coralyne chloride can be used for preparing coralyne derivatives as DNA binding fluorescent probes^[3].

Coralyne (6.25-100 μM; 24-72 hours) has cytotoxicity effect on breast cancer cell lines. It against MCF-7, MDA-MB-231 and MCF-10A cells with IC₅₀s of 76.4 uM, 76.4 uM, and 99 uM,respectively at 24 hours. And it against MCF-7, MDA-MB-231 and MCF-10A cells with IC₅₀s of 21.9 uM, 19.1 uM, and 91 μM, respectively at 72 hours^[1].
Coralyne (25 μM; 48 hours) significantly downregulates cancer cell attachment of MCF-7 and MDA-MB-231 compared to the untreated controls. The percent of reduction in adhesion of MCF-7 is 55%, whereas 53% in reduction in the adhesion of MDA-MB-23 and 62% in reduction of MCF-10A,respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

399.87

C₂₂H₂₂ClNO₄

38989-38-7

Solid

Light yellow to yellow

CC1=C2C=C(C(OC)=CC2=CC3=C4C=C(C(OC)=CC4=CC=[N+]13)OC)OC.[Cl-]

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Seema Kumari, et al. Synergistic effects of coralyne and paclitaxel on cell migration and proliferation of breast cancer cells lines. Biomed Pharmacother. 2017 Jul;91:436-445.  [Content Brief]

  [2]. D Makhey, et al. Coralyne and related compounds as mammalian topoisomerase I and topoisomerase II poisons. Bioorg Med Chem. 1996 Jun;4(6):781-91.  [Content Brief]

  [3]. P M Pithan, et al. 8-Styryl-substituted coralyne derivatives as DNA binding fluorescent probes.RSC Adv. 2017 Feb 8;7(18):10660-10667.  [Content Brief]