2. Epigenetics Apoptosis
  3. Histone Acetyltransferase Apoptosis
  4. CTB

CTB is a potent p300 histone acetyltransferase activator. CTB can effectively induce apoptosis in MCF-7 cells.

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CTB Chemical Structure

CTB Chemical Structure

CAS No. : 451491-47-7

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10 mM * 1 mL in DMSO
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Customer Review

Based on 3 publication(s) in Google Scholar

CTB Related Antibodies

Top Publications Citing Use of Products

View All Histone Acetyltransferase Isoform Specific Products:

View All Isoforms
CBP/p300 GCN5/PCAF TIP60 MOZ/MORF HBO1

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

CTB is a potent p300 histone acetyltransferase activator[1]. CTB can effectively induce apoptosis in MCF-7 cells[2].

IC50 & Target[1]

CBP/p300

 

In Vitro

CTB (10, 50, 100, 150, 200, and 250 μM; 10 min) enhances p300 HAT activity of p300 in a dosedependent manner[1].
CTB (0-200 μM; 24 hours) inhibits the viability of MCF-7 cells with an IC50 of 85.43 μM[2].
CTB (85.43 μM; 24, 48 and 72 h) induces time dependence apoptosis of MCF-7 cells[2].
CTB (50 uM; 24 h) increases p300/CBP activity , and reduces autophagic flux in primary neurons[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CTB Related Antibodies

Cell Viability Assay[2]

Cell Line: MCF-7 cell
Concentration: 0-200 μM
Incubation Time: 24 hours
Result: Inhibit viability with an IC50 of 85.43 μM.

Apoptosis Analysis[2]

Cell Line: MCF-7 cell
Concentration: 85.43 μM
Incubation Time: 24, 48 and 72 h
Result: Iinduced time-dependence apoptosis.
Clinical Trial
Molecular Weight

343.73

Formula

C16H13ClF3NO2
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=CC=C(Cl)C(C(F)(F)F)=C1)C2=CC=CC=C2OCC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (290.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9093 mL 14.5463 mL 29.0926 mL
5 mM 0.5819 mL 2.9093 mL 5.8185 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (7.27 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (7.27 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.93%

COA (249 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9093 mL 14.5463 mL 29.0926 mL 72.7315 mL
5 mM 0.5819 mL 2.9093 mL 5.8185 mL 14.5463 mL
10 mM 0.2909 mL 1.4546 mL 2.9093 mL 7.2732 mL
15 mM 0.1940 mL 0.9698 mL 1.9395 mL 4.8488 mL
20 mM 0.1455 mL 0.7273 mL 1.4546 mL 3.6366 mL
25 mM 0.1164 mL 0.5819 mL 1.1637 mL 2.9093 mL
30 mM 0.0970 mL 0.4849 mL 0.9698 mL 2.4244 mL
40 mM 0.0727 mL 0.3637 mL 0.7273 mL 1.8183 mL
50 mM 0.0582 mL 0.2909 mL 0.5819 mL 1.4546 mL
60 mM 0.0485 mL 0.2424 mL 0.4849 mL 1.2122 mL
80 mM 0.0364 mL 0.1818 mL 0.3637 mL 0.9091 mL
100 mM 0.0291 mL 0.1455 mL 0.2909 mL 0.7273 mL
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CTB Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CTB451491-47-7Histone AcetyltransferaseApoptosisHATsHATpotentneuronsMCF-7 cellcancerautophagic fluxapoptosisInhibitorinhibitorinhibit

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