2. JAK/STAT Signaling Protein Tyrosine Kinase/RTK
  3. EGFR
  4. Dacomitinib hydrate

Dacomitinib hydrate  (Synonyms: PF-00299804 hydrate; PF-299804 hydrate)

Cat. No.: HY-118263 Purity: 99.03%
SDS
COA
Handling Instructions Technical Support

Dacomitinib (PF-00299804) hydrate is an orally active, irreversible pan-ErbB inhibitor. Dacomitinib hydrate can be used in the research of cancers such as metastatic non-small cell lung cancer (NSCLC).

For research use only. We do not sell to patients.

Dacomitinib hydrate Chemical Structure

Dacomitinib hydrate Chemical Structure

CAS No. : 1042385-75-0

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Customer Review

Based on 21 publication(s) in Google Scholar

Other Forms of Dacomitinib hydrate:

Dacomitinib hydrate Related Antibodies

Top Publications Citing Use of Products

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EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Dacomitinib (PF-00299804) hydrate is an orally active, irreversible pan-ErbB inhibitor. Dacomitinib hydrate can be used in the research of cancers such as metastatic non-small cell lung cancer (NSCLC)[1].

Cellular Effect
Cell Line Type Value Description References
Sf9 IC50
46 nM
Compound: 25, PF-00299804
Inhibition of GST-tagged ErbB2 expressed in sf9 cells by ELISA
Inhibition of GST-tagged ErbB2 expressed in sf9 cells by ELISA
[PMID: 22621397]
Sf9 IC50
6 nM
Compound: 25, PF-00299804
Inhibition of GST-tagged EGFR expressed in sf9 cells by ELISA
Inhibition of GST-tagged EGFR expressed in sf9 cells by ELISA
[PMID: 22621397]
Sf9 IC50
74 nM
Compound: 25, PF-00299804
Inhibition of GST-tagged ErbB4 expressed in sf9 cells by ELISA
Inhibition of GST-tagged ErbB4 expressed in sf9 cells by ELISA
[PMID: 22621397]
Clinical Trial
Molecular Weight

487.95

Formula

C24H27ClFN5O3
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C(/C=C/CN1CCCCC1)NC2=CC3=C(C=C2OC)N=CN=C3NC4=CC=C(C(Cl)=C4)F.O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMF : 10 mg/mL (20.49 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0494 mL 10.2470 mL 20.4939 mL
5 mM 0.4099 mL 2.0494 mL 4.0988 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMF    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1 mg/mL (2.05 mM); Clear solution

  • Protocol 2

    Add each solvent one by one:  10% DMF    90% Corn Oil

    Solubility: ≥ 1 mg/mL (2.05 mM); Clear solution

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMF 1 mM 2.0494 mL 10.2470 mL 20.4939 mL 51.2348 mL
5 mM 0.4099 mL 2.0494 mL 4.0988 mL 10.2470 mL
10 mM 0.2049 mL 1.0247 mL 2.0494 mL 5.1235 mL
15 mM 0.1366 mL 0.6831 mL 1.3663 mL 3.4157 mL
20 mM 0.1025 mL 0.5123 mL 1.0247 mL 2.5617 mL
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Dacomitinib hydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dacomitinib1042385-75-0PF-00299804PF-299804PF00299804PF 00299804PF299804PF 299804EGFREpidermal growth factor receptorErbB-1HER1NSCLCcancertumorInhibitorinhibitorinhibit

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