Effusol

Name: Effusol
Brand: MedChemExpress
SKU: HY-N5130
Rating: 4.5 (1 reviews)

Cat. No.: HY-N5130 Purity: 99.83%: FAQs
Data Sheet SDS COA Handling Instructions

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Effusol, a phenolic constituent from Juncus effuses, exhibits potent scavenging activity for DPPH and ABTS radicals, with IC₅₀ values of 79 μM and 2.73 μM, respectively. Effusol rescues CA1 LTP attenuated by corticosterone, defending the hippocampal function against stress-induced cognitive decline.

For research use only. We do not sell to patients.

Effusol Chemical Structure

CAS No. : 73166-28-6

Size	Price	Stock	Quantity
1 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	22.7 μM Compound: 9	Inhibition of OAT3 (unknown origin) expressed in HEK293 cells assessed as reduction in 6-CF uptake measured after 5 mins by fluorescence assay Inhibition of OAT3 (unknown origin) expressed in HEK293 cells assessed as reduction in 6-CF uptake measured after 5 mins by fluorescence assay	[PMID: 30892891]
HEK293	IC₅₀	23.3 μM Compound: 9	Inhibition of OAT1 (unknown origin) expressed in HEK293 cells assessed as reduction in 6-CF uptake measured after 5 mins by fluorescence assay Inhibition of OAT1 (unknown origin) expressed in HEK293 cells assessed as reduction in 6-CF uptake measured after 5 mins by fluorescence assay	[PMID: 30892891]

Molecular Weight

252.31

Formula

C₁₇H₁₆O₂

CAS No.

73166-28-6

Appearance

Solid

Color

White to off-white

SMILES

OC(C(C)=C1CC2)=CC=C1C3=C2C=C(O)C=C3C=C

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (396.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9634 mL	19.8169 mL	39.6338 mL
5 mM	0.7927 mL	3.9634 mL	7.9268 mL
10 mM	0.3963 mL	1.9817 mL	3.9634 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.91 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (249 KB) HNMR (160 KB) RP-HPLC (260 KB)

Handling Instructions (2659 KB)

References

[1]. Kim, Ye Jin, et al. Antioxidant and Cytotoxic Activity of Compounds from the Stem of Juncus effuses. Journal of Applied Biological Chemistry. Volume 57 Issue 1 / Pages.47-51 / 2014 / 1976-0442(pISSN).

[2]. Haruna Tamano, et al. CA1 LTP Attenuated by Corticosterone Is Canceled by Effusol via Rescuing Intracellular Zn 2+ Dysregulation. Cell Mol Neurobiol. 2019 Oct;39(7):975-983. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.9634 mL	19.8169 mL	39.6338 mL	99.0845 mL
	5 mM	0.7927 mL	3.9634 mL	7.9268 mL	19.8169 mL
	10 mM	0.3963 mL	1.9817 mL	3.9634 mL	9.9084 mL
	15 mM	0.2642 mL	1.3211 mL	2.6423 mL	6.6056 mL
	20 mM	0.1982 mL	0.9908 mL	1.9817 mL	4.9542 mL
	25 mM	0.1585 mL	0.7927 mL	1.5854 mL	3.9634 mL
	30 mM	0.1321 mL	0.6606 mL	1.3211 mL	3.3028 mL
	40 mM	0.0991 mL	0.4954 mL	0.9908 mL	2.4771 mL
	50 mM	0.0793 mL	0.3963 mL	0.7927 mL	1.9817 mL
	60 mM	0.0661 mL	0.3303 mL	0.6606 mL	1.6514 mL
	80 mM	0.0495 mL	0.2477 mL	0.4954 mL	1.2386 mL
	100 mM	0.0396 mL	0.1982 mL	0.3963 mL	0.9908 mL

Effusol Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Effusol73166-28-6OthersInhibitorinhibitorinhibit

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Effusol

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Complete Stock Solution Preparation Table

Effusol Related Classifications

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