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  6. Enmein

Enmein is isolated from I. serra with immunosuppressive effect.

For research use only. We do not sell to patients.

Enmein Chemical Structure

Enmein Chemical Structure

CAS No. : 3776-39-4

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Based on 1 publication(s) in Google Scholar

Enmein Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

Enmein is isolated from I. serra with immunosuppressive effect[1].

In Vitro

Enmein shows significant inhibitory effect toward K562 with an IC50 value ranging from 3.2 μg/ml to 8.2 μg/ml[2].
Enmein (3.13-25 μg/ml; 48 hours) has inhibitory effect on lymphocytes with significant difference within concentration from 6.25 μg/ml to 25 μg/ml[1].
Enmein (5.5-22 μg/ml; 48 hours) causes the percentage of S stage cells decreased from 19% to 6%, causes the percentage of G2/M stage cells to decrease from 2% to 0.4%[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Enmein Related Antibodies

Cell Viability Assay[1]

Cell Line: Splenic lymphocyte cells
Concentration: 3.13, 6.25, 12.5, 25 μg/ml
Incubation Time: 48 hours
Result: Exhibited an IC50 value of 17 μg/ml in splenic lymphocytes.

Cell Cycle Analysis[1]

Cell Line: Splenic lymphocyte cells
Concentration: 5.5 μg/ml, 11 μg/ml, 22 μg/ml
Incubation Time: 48 hours
Result: Caused S stage cells and G2/M stage cells decreased.
In Vivo

Enmein (inject to ear; 6.52-13.04 mg/kg; 5 days) inhibits the xylene-induced ear swelling and inhibits IL-2 expression[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Balb/c mice[1]
Dosage: 6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg
Administration: Injected to ear; 6.52 mg/kg, 9.78 mg/kg or 13.04 mg/kg; 5 days
Result: Had inhibitory effect on IL-2 release in blood.
Molecular Weight

362.42

Formula

C20H26O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CC1([C@]2([H])[C@]3(CO[C@H]2O)[C@@]4([H])[C@@](C5=O)(C(O[C@@]3([H])C[C@@H]1O)=O)C[C@@](C5=C)([H])CC4)C
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (68.98 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7592 mL 13.7961 mL 27.5923 mL
5 mM 0.5518 mL 2.7592 mL 5.5185 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥98.0%

COA (260 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7592 mL 13.7961 mL 27.5923 mL 68.9807 mL
5 mM 0.5518 mL 2.7592 mL 5.5185 mL 13.7961 mL
10 mM 0.2759 mL 1.3796 mL 2.7592 mL 6.8981 mL
15 mM 0.1839 mL 0.9197 mL 1.8395 mL 4.5987 mL
20 mM 0.1380 mL 0.6898 mL 1.3796 mL 3.4490 mL
25 mM 0.1104 mL 0.5518 mL 1.1037 mL 2.7592 mL
30 mM 0.0920 mL 0.4599 mL 0.9197 mL 2.2994 mL
40 mM 0.0690 mL 0.3449 mL 0.6898 mL 1.7245 mL
50 mM 0.0552 mL 0.2759 mL 0.5518 mL 1.3796 mL
60 mM 0.0460 mL 0.2299 mL 0.4599 mL 1.1497 mL
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Enmein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Enmein3776-39-4OthersInhibitorinhibitorinhibit

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