Evocarpine

Name: Evocarpine
Brand: MedChemExpress
SKU: HY-N2060
Rating: 4.5 (1 reviews)

Cat. No.: HY-N2060 Purity: 99.73%: FAQs
Data Sheet SDS COA Handling Instructions

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Evocarpine, a quinolone alkaloid that could be isolated from Evodiae fructus, inhibitss Ca²⁺ influx through voltage-dependent calcium channels. Antimycobacterial activity.

For research use only. We do not sell to patients.

Evocarpine Chemical Structure

CAS No. : 15266-38-3

Size	Price	Stock	Quantity
10 mg	USD 220	In-stock	Estimated Time of Arrival: December 31
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100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

Evocarpine, a quinolone alkaloid that could be isolated from Evodiae fructus, inhibitss Ca²⁺ influx through voltage-dependent calcium channels. Antimycobacterial activity^[1]^[2].

In Vitro

Cumulative application of evocarpine (1-100 μM) inhibits the sustained contraction induced by 60 mM K⁺ in a concentration-dependent manner^[1].
Evocarpine is found to induce apoptotic cell death in promyelocytic leukaemia HL‐60 cells in dose- and time-dependent manners^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

339.51

Formula

C₂₃H₃₃NO

CAS No.

15266-38-3

Appearance

Solid

Color

White to off-white

SMILES

O=C1C=C(CCCCCCC/C=C\CCCC)N(C)C2=C1C=CC=C2

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (294.54 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9454 mL	14.7271 mL	29.4542 mL
5 mM	0.5891 mL	2.9454 mL	5.8908 mL
10 mM	0.2945 mL	1.4727 mL	2.9454 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.36 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.36 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.73%

Select Batch:

Data Sheet (267 KB) SDS (251 KB)

COA (240 KB) HNMR (155 KB) CNMR (152 KB) RP-HPLC (297 KB)

Handling Instructions (2659 KB)

References

[1]. J Yamahar, et al. The vasorelaxant effect of evocarpine in isolated aortic strips: mode of action. Eur J Pharmacol. 1988 Oct 11;155(1-2):139-43. [Content Brief]

[2]. C Hochfellner, et al. Antagonistic effects of indoloquinazoline alkaloids on antimycobacterial activity of evocarpine. J Appl Microbiol. 2015 Apr;118(4):864-72. [Content Brief]

[3]. N Y Kim, et al. Cyclic adenosine monophosphate inhibits quinolone alkaloid evocarpine-induced apoptosis via activation of protein kinase A in human leukaemic HL-60 cells. Pharmacol Toxicol. 2000 Jul;87(1):1-5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9454 mL	14.7271 mL	29.4542 mL	73.6355 mL
	5 mM	0.5891 mL	2.9454 mL	5.8908 mL	14.7271 mL
	10 mM	0.2945 mL	1.4727 mL	2.9454 mL	7.3636 mL
	15 mM	0.1964 mL	0.9818 mL	1.9636 mL	4.9090 mL
	20 mM	0.1473 mL	0.7364 mL	1.4727 mL	3.6818 mL
	25 mM	0.1178 mL	0.5891 mL	1.1782 mL	2.9454 mL
	30 mM	0.0982 mL	0.4909 mL	0.9818 mL	2.4545 mL
	40 mM	0.0736 mL	0.3682 mL	0.7364 mL	1.8409 mL
	50 mM	0.0589 mL	0.2945 mL	0.5891 mL	1.4727 mL
	60 mM	0.0491 mL	0.2455 mL	0.4909 mL	1.2273 mL
	80 mM	0.0368 mL	0.1841 mL	0.3682 mL	0.9204 mL
	100 mM	0.0295 mL	0.1473 mL	0.2945 mL	0.7364 mL

Evocarpine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Evocarpine15266-38-3ApoptosisBacterialCa2+InfluxleukaemiaapoptosisHL-60antimycobacterialInhibitorinhibitorinhibit

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Evocarpine

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Evocarpine Related Antibodies

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Complete Stock Solution Preparation Table

Evocarpine Related Classifications

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