2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. For-Met-OH

For-Met-OH  (Synonyms: N-Formylmethionine)

Cat. No.: HY-W016647 Purity: 99.92%
COA Handling Instructions

Molarity Calculator

For-Met-OH is an endogenous metabolite.

For research use only. We do not sell to patients.

For-Met-OH Chemical Structure

For-Met-OH Chemical Structure

CAS No. : 4289-98-9

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

For-Met-OH Related Antibodies

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Description

For-Met-OH is an endogenous metabolite.

IC50 & Target

Microbial Metabolite

 

Human Endogenous Metabolite

 

Molecular Weight

177.22

Formula

C6H11NO3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CSCC[C@@H](C(O)=O)NC=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (564.27 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 50 mg/mL (282.14 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.6427 mL 28.2135 mL 56.4270 mL
5 mM 1.1285 mL 5.6427 mL 11.2854 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 6.25 mg/mL (35.27 mM); Clear solution

    This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (14.11 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.92%

COA (246 KB) HNMR (249 KB) RP-HPLC (214 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 5.6427 mL 28.2135 mL 56.4270 mL 141.0676 mL
5 mM 1.1285 mL 5.6427 mL 11.2854 mL 28.2135 mL
10 mM 0.5643 mL 2.8214 mL 5.6427 mL 14.1068 mL
15 mM 0.3762 mL 1.8809 mL 3.7618 mL 9.4045 mL
20 mM 0.2821 mL 1.4107 mL 2.8214 mL 7.0534 mL
25 mM 0.2257 mL 1.1285 mL 2.2571 mL 5.6427 mL
30 mM 0.1881 mL 0.9405 mL 1.8809 mL 4.7023 mL
40 mM 0.1411 mL 0.7053 mL 1.4107 mL 3.5267 mL
50 mM 0.1129 mL 0.5643 mL 1.1285 mL 2.8214 mL
60 mM 0.0940 mL 0.4702 mL 0.9405 mL 2.3511 mL
80 mM 0.0705 mL 0.3527 mL 0.7053 mL 1.7633 mL
100 mM 0.0564 mL 0.2821 mL 0.5643 mL 1.4107 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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For-Met-OH Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

For-Met-OH4289-98-9N-FormylmethionineEndogenous MetaboliteInhibitorinhibitorinhibit

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