PYCARD - PYD and CARD domain containing Gene

Homo sapiens

Also known as ASC; TMS; TMS1; CARD5; TMS-1

Gene ID: 29108 | Gene type: protein coding

About PYCARD

Cytogenetic location: 16p11.2 Genomic coordinates (GRCh38): 16:31,201,486-31,202,760 (from NCBI)

This gene has 4 transcripts (splice variants), 139 orthologues and 16 paralogues. Broad expression in spleen (RPKM 25.5), colon (RPKM 25.4) and 22 other tissues.

Summary

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of Caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing Apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

PYCARD Products(2)

mRNA	Protein	Name
NM_013258.5	NP_037390.2	apoptosis-associated speck-like protein containing a CARD isoform a
NM_145182.3	NP_660183.1	apoptosis-associated speck-like protein containing a CARD isoform b

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	Reference	Source
enables BMP receptor binding	IPI IPI: Inferred from physical interaction	24407287	GOA
enables Pyrin domain binding	IPI IPI: Inferred from physical interaction	15030775	GOA
enables enzyme binding	IPI IPI: Inferred from physical interaction	24407287	GOA
enables identical protein binding	IDA IDA: Inferred from direct assay	34341353	GOA
enables identical protein binding	IPI IPI: Inferred from physical interaction	24630722	GOA
enables interleukin-6 receptor binding	IPI IPI: Inferred from physical interaction	24407287	GOA
enables myosin I binding	IPI IPI: Inferred from physical interaction	24407287	GOA
enables protease binding	IPI IPI: Inferred from physical interaction	24407287	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	11498534	GOA
enables protein dimerization activity	IDA IDA: Inferred from direct assay	24531343	GOA
enables protein homodimerization activity	IDA IDA: Inferred from direct assay	15030775	GOA
enables tropomyosin binding	IPI IPI: Inferred from physical interaction	24407287	GOA

Biological Process GO Annotation	Evidence	Reference	Source
involved in NLRP3 inflammasome complex assembly	IDA IDA: Inferred from direct assay	27432880	GOA
involved in activation of innate immune response	IDA IDA: Inferred from direct assay	21575908	GOA
involved in cellular response to interleukin-1	IDA IDA: Inferred from direct assay	12486103	GOA
involved in cellular response to lipopolysaccharide	IDA IDA: Inferred from direct assay	12486103	GOA
involved in cellular response to tumor necrosis factor	IDA IDA: Inferred from direct assay	12486103	GOA
involved in defense response to Gram-negative bacterium	IMP IMP: Inferred from mutant phenotype	16982856	GOA
involved in defense response to virus	IDA IDA: Inferred from direct assay	21575908	GOA
involved in intrinsic apoptotic signaling pathway by p53 class mediator	IMP IMP: Inferred from mutant phenotype	14730312	GOA
involved in intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator	IMP IMP: Inferred from mutant phenotype	14730312	GOA
involved in myeloid dendritic cell activation	IMP IMP: Inferred from mutant phenotype	22732093	GOA
involved in negative regulation of NF-kappaB transcription factor activity	IMP IMP: Inferred from mutant phenotype	16585594	GOA
involved in negative regulation of canonical NF-kappaB signal transduction	IDA IDA: Inferred from direct assay	12486103	GOA
involved in negative regulation of cytokine production involved in inflammatory response	IMP IMP: Inferred from mutant phenotype	24531343	GOA
involved in negative regulation of interferon-beta production	IMP IMP: Inferred from mutant phenotype	19158675	GOA
involved in negative regulation of protein serine/threonine kinase activity	IDA IDA: Inferred from direct assay	12486103	GOA
involved in positive regulation of DNA-binding transcription factor activity	IDA IDA: Inferred from direct assay	19494289	GOA
involved in positive regulation of ERK1 and ERK2 cascade	IMP IMP: Inferred from mutant phenotype	21487011	GOA
involved in positive regulation of JNK cascade	IMP IMP: Inferred from mutant phenotype	21487011	GOA
involved in positive regulation of NF-kappaB transcription factor activity	IDA IDA: Inferred from direct assay	12646168	GOA
involved in positive regulation of T cell activation	IMP IMP: Inferred from mutant phenotype	22732093	GOA
involved in positive regulation of adaptive immune response	IMP IMP: Inferred from mutant phenotype	22732093	GOA
involved in positive regulation of apoptotic process	IDA IDA: Inferred from direct assay	12646168	GOA
involved in positive regulation of canonical NF-kappaB signal transduction	IDA IDA: Inferred from direct assay	12656673	GOA
involved in positive regulation of chemokine production	IMP IMP: Inferred from mutant phenotype	21487011	GOA
involved in positive regulation of cysteine-type endopeptidase activity	IDA IDA: Inferred from direct assay	19158676	GOA
involved in positive regulation of cysteine-type endopeptidase activity involved in apoptotic process	IMP IMP: Inferred from mutant phenotype	22267217	GOA
involved in positive regulation of extrinsic apoptotic signaling pathway	IDA IDA: Inferred from direct assay	16964285	GOA
involved in positive regulation of inflammatory response	IDA IDA: Inferred from direct assay	12191486	GOA
involved in positive regulation of interleukin-1 beta production	IDA IDA: Inferred from direct assay	7642516	GOA
involved in positive regulation of interleukin-1 beta production	IMP IMP: Inferred from mutant phenotype	22267217	GOA
involved in positive regulation of interleukin-10 production	IMP IMP: Inferred from mutant phenotype	16982856	GOA
involved in positive regulation of interleukin-6 production	IMP IMP: Inferred from mutant phenotype	16982856	GOA
involved in positive regulation of interleukin-8 production	IMP IMP: Inferred from mutant phenotype	16982856	GOA
involved in positive regulation of non-canonical NF-kappaB signal transduction	IPI IPI: Inferred from physical interaction	15817483	GOA
involved in positive regulation of release of cytochrome c from mitochondria	IDA IDA: Inferred from direct assay	14730312	GOA
involved in positive regulation of tumor necrosis factor production	IMP IMP: Inferred from mutant phenotype	16982856	GOA
involved in protein homooligomerization	IDA IDA: Inferred from direct assay	24531343	GOA
involved in regulation of intrinsic apoptotic signaling pathway	IDA IDA: Inferred from direct assay	14730312	GOA
involved in regulation of tumor necrosis factor-mediated signaling pathway	IMP IMP: Inferred from mutant phenotype	14730312	GOA
involved in tumor necrosis factor-mediated signaling pathway	IDA IDA: Inferred from direct assay	12656673	GOA

Cellular Component GO Annotation	Evidence	Reference	Source
part of AIM2 inflammasome complex	IDA IDA: Inferred from direct assay	19158676	GOA
part of AIM2 inflammasome complex	IPI IPI: Inferred from physical interaction	24630722	GOA
located in Golgi membrane	IDA IDA: Inferred from direct assay	23229815	GOA
part of IkappaB kinase complex	IDA IDA: Inferred from direct assay	12486103	GOA
part of NLRP1 inflammasome complex	IDA IDA: Inferred from direct assay	12191486	GOA
part of NLRP1 inflammasome complex	IPI IPI: Inferred from physical interaction	17349957	GOA
part of NLRP3 inflammasome complex	IDA IDA: Inferred from direct assay	15030775	GOA
part of NLRP3 inflammasome complex	IPI IPI: Inferred from physical interaction	29255148	GOA
part of NLRP6 inflammasome complex	IDA IDA: Inferred from direct assay	30674671	GOA
part of canonical inflammasome complex	IPI IPI: Inferred from physical interaction	16037825	GOA
located in cytoplasm	IDA IDA: Inferred from direct assay	14730312	GOA
located in mitochondrion	IDA IDA: Inferred from direct assay	14730312	GOA
part of protein-containing complex	IDA IDA: Inferred from direct assay	22267217	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

PYCARD Protein Structure

PYRIN

CARD

0
100
195 a.a.

Protein Preferred Names

Protein Names

apoptosis-associated speck-like protein containing a CARD

caspase recruitment domain-containing protein 5

PYCARD Protein-protein interaction Information

Type Cross Intra	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	Reference
Intra	PYCARD	Q9ULZ3	RIPK1	Homo sapiens	Q13546	Anti Tag CoIP	25402006
Intra	PYCARD	Q9ULZ3	BAX	Homo sapiens	Q07812	Confocal	14730312
Intra	PYCARD	Q9ULZ3	CASP1	Homo sapiens	P29466	Anti Bait CoIP	31421660
Intra	PYCARD	Q9ULZ3	CASP1	Homo sapiens	P29466	Anti Bait CoIP	34589402
Intra	PYCARD	Q9ULZ3	CASP1	Homo sapiens	P29466	Anti Bait CoIP	24630722
Intra	PYCARD	Q9ULZ3	CASP1	Homo sapiens	P29466	Anti Bait CoIP	25605870
Intra	PYCARD	Q9ULZ3	AIM2	Homo sapiens	O14862	Pull Down	19158676
Intra	PYCARD	Q9ULZ3	AIM2	Homo sapiens	O14862	EM	24630722
Intra	PYCARD	Q9ULZ3	AIM2	Homo sapiens	O14862	FPS	24630722
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	EM	24630722
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	Anti Bait CoIP	31421660
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	Y2H	11786556
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	Confocal	34341353
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	Anti Tag CoIP	34341353
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	IF	11786556
Intra	PYCARD	Q9ULZ3	NLRP3	Homo sapiens	Q96P20	Anti Tag CoIP	19158675
Intra	PYCARD	Q9ULZ3	PYDC2	Homo sapiens	Q56P42	Pull Down	17178784
Intra	PYCARD	Q9ULZ3	PYDC2	Homo sapiens	Q56P42	Anti Tag CoIP	17178784
Intra	PYCARD	Q9ULZ3	MEFV	Homo sapiens	O15553	Pull Down	25006247
Intra	PYCARD	Q9ULZ3	MEFV	Homo sapiens	O15553	CoIP	11498534
Intra	PYCARD	Q9ULZ3	MEFV	Homo sapiens	O15553	Y2H	11498534
Intra	PYCARD	Q9ULZ3	SYK	Homo sapiens	P43405	Protein Kinase Assay	25605870
Intra	PYCARD	Q9ULZ3	SYK	Homo sapiens	P43405	Anti Bait CoIP	25605870
Intra	PYCARD	Q9ULZ3	SYK	Homo sapiens	P43405	Anti Tag CoIP	25605870
Intra	PYCARD	Q9ULZ3	EXOC5	Homo sapiens	O00471	Y2H Prey Pooling	25416956
Intra	PYCARD	Q9ULZ3	EXOC5	Homo sapiens	O00471	Y2H Array	25416956

Cross: Cross-species interaction Intra: Intraspecies interaction

Related Diseases

Diseases

Alias

Muckle-Wells Syndrome

MWS	Urticaria-Deafness-Amyloidosis Syndrome
Uda Syndrome	Neutrophilic Urticaria
Urticaria, Deafness And Amyloidosis	Cryopyrin-Associated Periodic Syndrome 2
Caps2	Muckle Wells Syndrome
Urticaria-Deafness-Amyloidosis	Cryopyrin-Associated Periodic Syndromes

Cinca Syndrome

CINCA	Nomid
Cryopyrin-Associated Periodic Syndrome 3	Chronic Neurologic Cutaneous And Articular Syndrome
Multisystem Inflammatory Disease, Neonatal-Onset	Caps3
Chronic Infantile Neurological Cutaneous Articular Syndrome	Infantile-Onset Multisystem Inflammatory Disease
Iomid Syndrome	Neonatal-Onset Multisystem Inflammatory Disease
Nomid Syndrome	Prieur-Griscelli Syndrome
Neonatal Onset Multisystem Inflammatory Disease	Chronic Infantile Neurological, Cutaneous And Articular Syndrome
Iomid	Infantile Onset Multisystem Inflammatory Disease
Prieur Griscelli Syndrome	Chronic Infantile Neurological Cutaneous And Articular Syndrome
Chronic, Infantile, Neurological, Cutaneous, Articular Syndrome	Chronic Infantile Neurologic Cutaneous And Articular Syndrome
Chronic Infantile Neurological, Cutaneous, And Articular Syndrome	Cryopyrin-Associated Periodic Syndromes

Familial Mediterranean Fever

Periodic Fever Syndrome	FMF
Benign Paroxysmal Peritonitis	Periodic Disease
Recurrent Polyserositis	Familial Paroxysmal Polyserositis
Periodic Fever	Familial Mediterranean Fever, Autosomal Recessive
Familial Mediterranean Fever, Ar	Polyserositis, Recurrent
Polyserositis, Familial Paroxysmal	Periodic Peritonitis
Mef	Reimann Periodic Disease
Siegal-Cattan-Mamou Disease	Wolff Periodic Disease
Benign Recurrent Polyserositis	Mediterranean Fever, Familial
ARFMF	Autosomal Recessive Familial Mediterranean Fever
Fever, Mediterranean, Familial, Autosomal Recessive	Hereditary Autoinflammatory Diseases
Fmf - [Familial Mediterranean Fever]	Periodic Polyserositis
Periodic Familial Polyserositis	Periodic Familial Peritonitis
Paroxysmal Polyserositis	Hereditary Amyloid Nephropathy
Familial Recurrent Polyserositis	Familial Non-Neuropathic Amyloidosis
Armenian Disease	Riemann Periodic Disease
Siegal Cattan Mamou Disease

Osteomyelitis

Gout

Gouty Arthritis	Articular Gout
Gouty Arthropathy	Arthritis, Gouty
Arthritis Gouty	Idiopathic Gout
Idiopathic Gout, Unspecified Site	Gouty Bursitis
Uratic Arthritis	Gout Nos
Gouty	Gouty Diathesis

Familial Cold Autoinflammatory Syndrome

Familial Cold Urticaria	Fcas
Familial Polymorphous Cold Eruption	Fcu
Cold Hypersensitivity

Melanoma

Malignant Melanoma	Cutaneous Melanoma
Naevocarcinoma	Malignant Melanomas

Colorectal Cancer

Colon Cancer	Colorectal Carcinoma
Colon Carcinoma	Colorectal Cancer, Susceptibility To
Carcinoma Of Colon	CRC
Colorectal Cancer With Chromosomal Instability, Somatic	Colon Cancer, Somatic
Colon Cancer, Susceptibility To	Colonic Neoplasms
Colorectal Neoplasms	Colorectal Cancer, Somatic
Colon Cancer, Advanced, Somatic	Colonic Carcinoma
Colorectal Carcinomas	Colon Cancers
Colorectal Cancers	Cancer, Colorectal, Somatic
Cancer, Colon	Cancer, Colorectal, Susceptibility To
Colorectal Neoplasm	Colonic Neoplasm
Malignant Tumor Of Colon

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Bioactive Molecules (1)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS11485	PYCARD Human Pre-designed siRNA Set A	Pre-designed Sets	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Mus musculus	PYCARD	MGD	MGI:1931465
Macaca mulatta	PYCARD	VGNC	VGNC:76489
Felis catus	PYCARD	VGNC	VGNC:80676
Rattus norvegicus	PYCARD	RGD	RGD:628637
Others	PYCARD	NCBI

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