2. Anti-infection
  3. Bacterial
  4. Gut restricted-7

Gut restricted-7  (Synonyms: GR-7)

Cat. No.: HY-135747 Purity: 98.23%
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Gut restricted-7 (GR-7) is a potent, covalent and orally active pan-bile salt hydrolase (BSH) inhibitor. Gut restricted-7 has a tissue-selective and is restricted to the gut. Gut restricted-7 decreases gut bacterial BSHs and decreases deconjugated bile acid levels in feces of mice.

For research use only. We do not sell to patients.

Gut restricted-7 Chemical Structure

Gut restricted-7 Chemical Structure

CAS No. : 2553218-46-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 449 In-stock
Solution
10 mM * 1 mL in DMSO USD 449 In-stock
Solid
5 mg USD 400 In-stock
10 mg USD 640 In-stock
50 mg USD 1900 In-stock
100 mg USD 2850 In-stock
200 mg   Get quote  
500 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Gut restricted-7:

Gut restricted-7 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Gut restricted-7 (GR-7) is a potent, covalent and orally active pan-bile salt hydrolase (BSH) inhibitor. Gut restricted-7 has a tissue-selective and is restricted to the gut. Gut restricted-7 decreases gut bacterial BSHs and decreases deconjugated bile acid levels in feces of mice[1].

IC50 & Target

Bile salt hydrolase (BSH)[1]
In Vitro

BSH Inhibitor Gut restricted-7 (GR-7) (60 μM) does not affect epithelial barrier integrity of Caco-2 cells, suggesting that this compound is relatively nontoxic. Gut restricted-7 also does not affect microbial biomass[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Gut restricted-7 Related Antibodies
In Vivo

The C57BL/6 mice fed with either powdered chow containing 0.09% Gut restricted-7 (GR-7) (w/w) for 1 d day or powdered chow alone. Gut restricted-7 significant inhibits the BSH activity in the feces of inhibitortreated mice 8 h postdiet change. 20picomol/mg wet mass (~20 μM) of Gut restricted-7 in cecal contents is detected[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

510.63

Formula

C25H40FNaO6S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(CF)CC[C@@H](C)[C@@]1([H])CC[C@@]2([H])[C@]3([H])[C@H](O)C[C@]4([H])C[C@H](OS(=O)(O[Na])=O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 233.33 mg/mL (456.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 100 mg/mL (195.84 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9584 mL 9.7918 mL 19.5837 mL
5 mM 0.3917 mL 1.9584 mL 3.9167 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.19 mg/mL (4.29 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.19 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.9 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.07 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.9584 mL 9.7918 mL 19.5837 mL 48.9591 mL
5 mM 0.3917 mL 1.9584 mL 3.9167 mL 9.7918 mL
10 mM 0.1958 mL 0.9792 mL 1.9584 mL 4.8959 mL
15 mM 0.1306 mL 0.6528 mL 1.3056 mL 3.2639 mL
20 mM 0.0979 mL 0.4896 mL 0.9792 mL 2.4480 mL
25 mM 0.0783 mL 0.3917 mL 0.7833 mL 1.9584 mL
30 mM 0.0653 mL 0.3264 mL 0.6528 mL 1.6320 mL
40 mM 0.0490 mL 0.2448 mL 0.4896 mL 1.2240 mL
50 mM 0.0392 mL 0.1958 mL 0.3917 mL 0.9792 mL
60 mM 0.0326 mL 0.1632 mL 0.3264 mL 0.8160 mL
80 mM 0.0245 mL 0.1224 mL 0.2448 mL 0.6120 mL
100 mM 0.0196 mL 0.0979 mL 0.1958 mL 0.4896 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Gut restricted-7 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Gut restricted-72553218-46-3GR-7Gut restricted7Gut restricted 7GR7GR 7BacterialNontoxicBSHgutbileacidorallygut-restrictedbacterialInhibitorinhibitorinhibit

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