2. Metabolic Enzyme/Protease
  3. Ser/Thr Protease
  4. Hepln-13

Hepln-13 is a potent and orally active Hepsin inhibitor with an IC50 of 0.33 μM. Hepln-13 can be used for the research of metastatic prostate cancer.

For research use only. We do not sell to patients.

Hepln-13 Chemical Structure

Hepln-13 Chemical Structure

CAS No. : 64369-13-7

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 180 In-stock
Solution
10 mM * 1 mL in DMSO USD 180 In-stock
Solid
5 mg USD 160 In-stock
10 mg USD 260 In-stock
25 mg USD 520 In-stock
50 mg USD 850 In-stock
100 mg   Get quote  
200 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Hepln-13 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Hepln-13 is a potent and orally active Hepsin inhibitor with an IC50 of 0.33 μM. Hepln-13 can be used for the research of metastatic prostate cancer[1].

IC50 & Target

IC50: 0.33 μM (Hepsin)
In Vitro

Hepln-13 (10 μM; 2 hours; 293 cells) inhibits cleavage[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Hepln-13 Related Antibodies

Western Blot Analysis[1]

Cell Line: 293 cells
Concentration: 10 µM
Incubation Time: 2 hours
Result: Inhibited cleavage.
In Vivo

HepIn-13 displays dose dependent inhibition of Hepsin overexpression-relevant prostate cancer phenotypes and blocks prostate cancer metastasis[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

325.20

Formula

C17H13BrN2
CAS No.
Appearance

Solid

Color

Pale purple to purple

SMILES

BrC1=CC=C(C=C1)C(N2)NC3=C4C2=CC=CC4=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (192.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.0750 mL 15.3752 mL 30.7503 mL
5 mM 0.6150 mL 3.0750 mL 6.1501 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (6.40 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.34%

SDS (393 KB)

COA (248 KB) HNMR (254 KB) LCMS (87 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.0750 mL 15.3752 mL 30.7503 mL 76.8758 mL
5 mM 0.6150 mL 3.0750 mL 6.1501 mL 15.3752 mL
10 mM 0.3075 mL 1.5375 mL 3.0750 mL 7.6876 mL
15 mM 0.2050 mL 1.0250 mL 2.0500 mL 5.1251 mL
20 mM 0.1538 mL 0.7688 mL 1.5375 mL 3.8438 mL
25 mM 0.1230 mL 0.6150 mL 1.2300 mL 3.0750 mL
30 mM 0.1025 mL 0.5125 mL 1.0250 mL 2.5625 mL
40 mM 0.0769 mL 0.3844 mL 0.7688 mL 1.9219 mL
50 mM 0.0615 mL 0.3075 mL 0.6150 mL 1.5375 mL
60 mM 0.0513 mL 0.2563 mL 0.5125 mL 1.2813 mL
80 mM 0.0384 mL 0.1922 mL 0.3844 mL 0.9609 mL
100 mM 0.0308 mL 0.1538 mL 0.3075 mL 0.7688 mL
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Hepln-13 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hepln-1364369-13-7Hepln13Hepln 13Ser/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasespotentnon-toxicorallyprostatecancerphenotypesoverexpression-relevantInhibitorinhibitorinhibit

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