2. Immunology/Inflammation Apoptosis
  3. Interleukin Related TNF Receptor NOD-like Receptor (NLR)
  4. HMGB1-IN-1

HMGB1-IN-1 (compound 6) displays strong NO inhibitory effect in RAW264.7 cells with IC50 value of 15.9 ± 0.6 μM. HMGB1-IN-1 inhibit the HMGB1/NF-κB/NLRP3 pathway. HMGB1-IN-1 shows good anti-inflammatory activity and good anti-sepsis effects in kidney injury.

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HMGB1-IN-1 Chemical Structure

HMGB1-IN-1 Chemical Structure

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Based on 1 publication(s) in Google Scholar

HMGB1-IN-1 Related Antibodies

Top Publications Citing Use of Products

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View All NOD-like Receptor (NLR) Isoform Specific Products:

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NLRP3 NLRP1 NOD1 NOD2

  • Biological Activity

  • Purity & Documentation

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Description

HMGB1-IN-1 (compound 6) displays strong NO inhibitory effect in RAW264.7 cells with IC50 value of 15.9 ± 0.6 μM. HMGB1-IN-1 inhibit the HMGB1/NF-κB/NLRP3 pathway. HMGB1-IN-1 shows good anti-inflammatory activity and good anti-sepsis effects in kidney injury[1].

IC50 & Target

IL-1β

 

NLRP3

 

In Vitro

HMGB1-IN-1 (compound 6) (0-30 μM, 0-48 h) not only decreases IL-1β and TNF-α levels in RAW264.7 cells and HK-2 cells, but also down-regulates the levels of NLRP3, P-NF-κB p65 and HMGB1 in activated HK-2 cells in a dose-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HMGB1-IN-1 Related Antibodies
In Vivo

HMGB1-IN-1 (compound 6) (15-30 mg/kg, intraperitoneal injection) shows good anti-inflammatory activity[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 mice (8-10 weeks old, 20±5 g)[1]
Dosage: 15, 30 mg/kg
Administration: Intraperitoneal injection, once a day for 7 consecutive days
Result: Decreased the expression levels of IL-1β to 70.1% at 15 mg/kg, and further decreased to 31.4% at 30 mg/kg. Downregulated TNF-α to 37.3%.
Molecular Weight

1042.22

Formula

C57H75N3O15
Appearance

Solid

Color

White to off-white

SMILES

O=C(C=C1[C@@]2([C@@](CC[C@@]31C)(CC[C@@](C)(C2)C(OCC4=CN([C@@H]5O[C@H](COC(C)=O)[C@H](OC(C)=O)[C@H](OC(C)=O)[C@H]5OC(C)=O)N=N4)=O)C)[H])[C@@]6([C@@](CC[C@@H](C7(C)C)OC(/C=C/C8=CC=C(OC)C=C8)=O)(C7CC[C@@]36C)C)[H]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (119.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9595 mL 4.7975 mL 9.5949 mL
5 mM 0.1919 mL 0.9595 mL 1.9190 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.9595 mL 4.7975 mL 9.5949 mL 23.9873 mL
5 mM 0.1919 mL 0.9595 mL 1.9190 mL 4.7975 mL
10 mM 0.0959 mL 0.4797 mL 0.9595 mL 2.3987 mL
15 mM 0.0640 mL 0.3198 mL 0.6397 mL 1.5992 mL
20 mM 0.0480 mL 0.2399 mL 0.4797 mL 1.1994 mL
25 mM 0.0384 mL 0.1919 mL 0.3838 mL 0.9595 mL
30 mM 0.0320 mL 0.1599 mL 0.3198 mL 0.7996 mL
40 mM 0.0240 mL 0.1199 mL 0.2399 mL 0.5997 mL
50 mM 0.0192 mL 0.0959 mL 0.1919 mL 0.4797 mL
60 mM 0.0160 mL 0.0800 mL 0.1599 mL 0.3998 mL
80 mM 0.0120 mL 0.0600 mL 0.1199 mL 0.2998 mL
100 mM 0.0096 mL 0.0480 mL 0.0959 mL 0.2399 mL
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HMGB1-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HMGB1-IN-1Interleukin RelatedTNF ReceptorNOD-like Receptor (NLR)ILTumor Necrosis Factor ReceptorTNFRHK-2RAW264.7 cellskidney injuryanti-inflammatoryanti-sepsisInhibitorinhibitorinhibit

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