IDO1/2-IN-1

Cat. No.: HY-145280 Purity: 98.06%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

IDO1/2-IN-1 (compound 4t) is the first potent IDO1/IDO2 dual inhibitor with IC₅₀s of 28 nM and 144 nM for IDO1 and IDO2, respectively. IDO1/2-IN-1 exhibits antitumor activies. Orally active.

For research use only. We do not sell to patients.

IDO1/2-IN-1 Chemical Structure

CAS No. : 2310286-45-2

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 662	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 662	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 620	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 980	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1950	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 3250	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of IDO1/2-IN-1:

IDO1/2-IN-1 hydrochloride Get quote

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View All Isoforms

IDO1 IDO2 IDO

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

IDO1

28 nM (IC₅₀)

IDO2

144 nM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
CT26	IC₅₀	61.96 μM Compound: 4t	Cytotoxicity against mouse CT26 cells assessed as reduction in cell viability by CCK8 assay Cytotoxicity against mouse CT26 cells assessed as reduction in cell viability by CCK8 assay	[PMID: 34854662]
HCT-116	IC₅₀	56.13 μM Compound: 4t	Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability by CCK8 assay Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability by CCK8 assay	[PMID: 34854662]
HT-29	IC₅₀	8325 μM Compound: 4t	Cytotoxicity against human HT-29 cells assessed as reduction in cell viability by CCK8 assay Cytotoxicity against human HT-29 cells assessed as reduction in cell viability by CCK8 assay	[PMID: 34854662]

Molecular Weight

485.27

Formula

C₁₆H₁₈BrFN₈O₄

CAS No.

2310286-45-2

Appearance

Solid

Color

Off-white to light yellow

SMILES

FC1=CC=C(C=C1Br)/N=C(C2=NON=C2NCCN3N=NC(COCCO)=C3)/NO

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (206.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0607 mL	10.3035 mL	20.6071 mL
5 mM	0.4121 mL	2.0607 mL	4.1214 mL
10 mM	0.2061 mL	1.0304 mL	2.0607 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.06%

Select Batch:

Data Sheet (270 KB) SDS (251 KB)

COA (249 KB) HNMR (331 KB) RP-HPLC (239 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. He X, et al. Discovery of the First Potent IDO1/IDO2 Dual Inhibitors: A Promising Strategy for Cancer Immunotherapy. J Med Chem. 2021;64(24):17950-17968. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0607 mL	10.3035 mL	20.6071 mL	51.5177 mL
	5 mM	0.4121 mL	2.0607 mL	4.1214 mL	10.3035 mL
	10 mM	0.2061 mL	1.0304 mL	2.0607 mL	5.1518 mL
	15 mM	0.1374 mL	0.6869 mL	1.3738 mL	3.4345 mL
	20 mM	0.1030 mL	0.5152 mL	1.0304 mL	2.5759 mL
	25 mM	0.0824 mL	0.4121 mL	0.8243 mL	2.0607 mL
	30 mM	0.0687 mL	0.3435 mL	0.6869 mL	1.7173 mL
	40 mM	0.0515 mL	0.2576 mL	0.5152 mL	1.2879 mL
	50 mM	0.0412 mL	0.2061 mL	0.4121 mL	1.0304 mL
	60 mM	0.0343 mL	0.1717 mL	0.3435 mL	0.8586 mL
	80 mM	0.0258 mL	0.1288 mL	0.2576 mL	0.6440 mL
	100 mM	0.0206 mL	0.1030 mL	0.2061 mL	0.5152 mL

IDO1/2-IN-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IDO1/2-IN-12310286-45-2Indoleamine 2,3-Dioxygenase (IDO)antitumorCT26 xenograft mouse modelsIDO1IDO2oralInhibitorinhibitorinhibit

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IDO1/2-IN-1

Customer Review

Other Forms of IDO1/2-IN-1:

IDO1/2-IN-1 Related Antibodies

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•Related Screening Libraries:

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View All Indoleamine 2,3-Dioxygenase (IDO) Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

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Dilution Calculator

Complete Stock Solution Preparation Table

IDO1/2-IN-1 Related Classifications

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