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  4. Iohexol

Iohexol is a non-ionic, water-soluble contrast agent used as a reference marker for the determination of glomerular filtration rate (GFR) at the level of renal function. Iohexol can be used for contrast in myelography, computerized tomography (cisternography, ventriculography) and MicroCT imaging.

For research use only. We do not sell to patients.

Iohexol Chemical Structure

Iohexol Chemical Structure

CAS No. : 66108-95-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Iohexol:

Iohexol Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

Iohexol is a non-ionic, water-soluble contrast agent used as a reference marker for the determination of glomerular filtration rate (GFR) at the level of renal function. Iohexol can be used for contrast in myelography, computerized tomography (cisternography, ventriculography) and MicroCT imaging[1][2].

In Vitro

Iohexol (150 mg I/mL, 0-24 h) inhibits the cell viability of NRK 52-E[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Iohexol Related Antibodies

Cell Viability Assay[3]

Cell Line: NRK 52-E
Concentration: 150 mg I/mL
Incubation Time: 0-24 h
Result: Reduced the MTT conversion at 12 and 24 h.
Inhibited mitochondrial dehydrogenase activity at 12 and 24 h.
In Vivo

Iohexol (0.25-4 mL/kg, p.o., weekly) promotes the differentiation of dogs intestinal segments after application of 0.5-2.0 mL/kg[4].
Iohexol (64.7 mg/kg, i.v.) can be used to evaluate glomerular filtration rate (GFR) in healthy or dogs with presenting chronic kidney disease (CKD)[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Healthy dogs [4]
Dosage: 0.25-4 mL/kg
Administration: Oral gavage (p.o.), weekly
Result: Peaked the iohexol serum concentrations between 2 and 4 hours.
Reduced radiolucency after application of 0.25 mL/kg and 4 mL/kg.
Included watery diarrhea after ingestion of 4 mL/kg.
Clinical Trial
Molecular Weight

821.14

Formula

C19H26I3N3O9
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=C(I)C(N(C(C)=O)CC(O)CO)=C(I)C(C(NCC(O)CO)=O)=C1I)NCC(O)CO
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : ≥ 50 mg/mL (60.89 mM)

DMSO : 50 mg/mL (60.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2178 mL 6.0891 mL 12.1782 mL
5 mM 0.2436 mL 1.2178 mL 2.4356 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.04 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.04 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 120 mg/mL (146.14 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.20%

COA (249 KB) HNMR (106 KB) RP-HPLC (77 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 1.2178 mL 6.0891 mL 12.1782 mL 30.4455 mL
5 mM 0.2436 mL 1.2178 mL 2.4356 mL 6.0891 mL
10 mM 0.1218 mL 0.6089 mL 1.2178 mL 3.0445 mL
15 mM 0.0812 mL 0.4059 mL 0.8119 mL 2.0297 mL
20 mM 0.0609 mL 0.3045 mL 0.6089 mL 1.5223 mL
25 mM 0.0487 mL 0.2436 mL 0.4871 mL 1.2178 mL
30 mM 0.0406 mL 0.2030 mL 0.4059 mL 1.0148 mL
40 mM 0.0304 mL 0.1522 mL 0.3045 mL 0.7611 mL
50 mM 0.0244 mL 0.1218 mL 0.2436 mL 0.6089 mL
60 mM 0.0203 mL 0.1015 mL 0.2030 mL 0.5074 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Iohexol Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Iohexol66108-95-0AutophagyMitophagyMitochondrial AutophagyContrast AgentradiographicX-raysMicroCT imagingcisternographyventriculographymyelographyglomerular filtration rate (GFR)Inhibitorinhibitorinhibit

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