JS-K

Cat. No.: HY-126193 Purity: 99.59%: FAQs
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JS-K is a NO donor that reacts with glutathione to generate NO at physiological pH. JS-K inhibits proliferation, induces apoptosis, and disrupts the cell cycle of Jurkat T acute lymphoblastic leukemia cells.

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JS-K Chemical Structure

CAS No. : 205432-12-8

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1 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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nNOS iNOS eNOS

Biological Activity
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References
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Description

JS-K is a NO donor that reacts with glutathione to generate NO at physiological pH^[1]. JS-K inhibits proliferation, induces apoptosis, and disrupts the cell cycle of Jurkat T acute lymphoblastic leukemia cells^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
786-0	IC₅₀	0.8 μM Compound: JS-K	Antiproliferative activity against human 786O cells after 72 hrs by MTT assay Antiproliferative activity against human 786O cells after 72 hrs by MTT assay	[PMID: 18930407]
A2780	IC₅₀	0.63 μM Compound: 19	Antiproliferative activity against human A2780 cells after 48 hrs by MTT assay Antiproliferative activity against human A2780 cells after 48 hrs by MTT assay	[PMID: 25350923]
A549	IC₅₀	0.69 μM Compound: 6; JS-K	Antiproliferative activity against human A549 cells harboring KRAS G12S mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay Antiproliferative activity against human A549 cells harboring KRAS G12S mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]
A549	IC₅₀	1.26 μM Compound: 19	Antiproliferative activity against human A549 cells after 48 hrs by MTT assay Antiproliferative activity against human A549 cells after 48 hrs by MTT assay	[PMID: 25350923]
A549	IC₅₀	1.5 μM Compound: JS-K	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 18930407]
A549	IC₅₀	2.11 μM Compound: JS-K	Anticancer activity against human A549 cells assessed as inhibition of cell growth after 72 hrs by MTT or MTS assay Anticancer activity against human A549 cells assessed as inhibition of cell growth after 72 hrs by MTT or MTS assay	[PMID: 32156499]
A549	IC₅₀	2.313 μM Compound: JS-K	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 29501947]
A549	IC₅₀	6.53 μM Compound: JS-K	Antiproliferative activity against human A549 cells after 48 hrs by MTT assay relative to control Antiproliferative activity against human A549 cells after 48 hrs by MTT assay relative to control	[PMID: 29220794]
A549/TR	IC₅₀	1.987 μM Compound: JS-K	Antiproliferative activity against human A549/TR cells after 72 hrs by MTT assay Antiproliferative activity against human A549/TR cells after 72 hrs by MTT assay	[PMID: 29501947]
B16	IC₅₀	1340 nM Compound: 1; JS-K	Antiproliferative activity against mouse B16 cells after 72 hrs by MTT assay Antiproliferative activity against mouse B16 cells after 72 hrs by MTT assay	[PMID: 29420887]
Bel-7402	IC₅₀	5.17 μM Compound: JS-K	Cytotoxicity against human Bel7402 cells after 72 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 23617697]
BT-474	IC₅₀	3.66 μM Compound: JS-K	Anticancer activity against human BT474 cells assessed as inhibition of cell growth after 72 hrs by MTT or MTS assay Anticancer activity against human BT474 cells assessed as inhibition of cell growth after 72 hrs by MTT or MTS assay	[PMID: 32156499]
Caco-2	IC₅₀	2.6 μM Compound: JS-K	Antiproliferative activity against human Caco-2 cells after 72 hrs by MTT assay Antiproliferative activity against human Caco-2 cells after 72 hrs by MTT assay	[PMID: 18930407]
CCRF-CEM	IC₅₀	0.89 μM Compound: JS-K	Anticancer activity against human CCRF-CEM cells assessed as inhibition of cell growth after 72 hrs by MTT or MTS assay Anticancer activity against human CCRF-CEM cells assessed as inhibition of cell growth after 72 hrs by MTT or MTS assay	[PMID: 32156499]
HCT-116	IC₅₀	0.5 μM Compound: 6; JS-K	Antiproliferative activity against human HCT116 cells harboring KRAS G13D mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay Antiproliferative activity against human HCT116 cells harboring KRAS G13D mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]
HCT-116	IC₅₀	0.7 μM Compound: JS-K	Antiproliferative activity against human HCT 116 cells after 72 hrs by MTT assay Antiproliferative activity against human HCT 116 cells after 72 hrs by MTT assay	[PMID: 18930407]
HCT-116	IC₅₀	3.75 μM Compound: JS-K	Cytotoxicity against human HCT116 cells assessed as inhibition of cell proliferation after 48 hrs by MTT assay Cytotoxicity against human HCT116 cells assessed as inhibition of cell proliferation after 48 hrs by MTT assay	10.1039/C5MD00158G
HCT-116	IC₅₀	4.86 μM Compound: JS-K	Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay relative to control Antiproliferative activity against human HCT116 cells after 48 hrs by MTT assay relative to control	[PMID: 29220794]
HCT-15	IC₅₀	0.6 μM Compound: JS-K	Antiproliferative activity against human HCT-15 cells after 72 hrs by MTT assay Antiproliferative activity against human HCT-15 cells after 72 hrs by MTT assay	[PMID: 18930407]
HeLa	IC₅₀	3.56 μM Compound: 19	Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells after 48 hrs by MTT assay	[PMID: 25350923]
HepG2	IC₅₀	12.2 μM Compound: JS-K	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 23617697]
HepG2	IC₅₀	5.19 μM Compound: JS-K	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 23617697]
HepG2	IC₅₀	7.12 μM Compound: JS-K	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 23617697]
HepG2	IC₅₀	7.42 μM Compound: JS-K	Cytotoxicity against human HepG2 cells assessed as inhibition of cell proliferation after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as inhibition of cell proliferation after 48 hrs by MTT assay	10.1039/C5MD00158G
HL-60	IC₅₀	0.2 μM Compound: JS-K	Antiproliferative activity against human HL60 cells assessed as decrease in cell viability measured after 3 days by MTT assay Antiproliferative activity against human HL60 cells assessed as decrease in cell viability measured after 3 days by MTT assay	[PMID: 28505442]
HL-60	IC₅₀	0.2 μM Compound: JS-K	Antiproliferative activity against human HL-60 cells after 72 hrs by MTT assay Antiproliferative activity against human HL-60 cells after 72 hrs by MTT assay	[PMID: 18930407]
HL-60	IC₅₀	0.2 μM Compound: JS-K	Antiproliferative activity against human HL60 cells after 24 hrs Antiproliferative activity against human HL60 cells after 24 hrs	[PMID: 19364650]
HL-60	IC₅₀	0.53 μM Compound: 6; JS-K	Antiproliferative activity against human HL60 cells harboring NRAS K61L mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay Antiproliferative activity against human HL60 cells harboring NRAS K61L mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]
HT-29	IC₅₀	0.8 μM Compound: JS-K	Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 18930407]
hTERT-BJ	IC₅₀	10 μM Compound: JS-K	Antiproliferative activity against human BJ-5ta cells after 72 hrs by MTT assay Antiproliferative activity against human BJ-5ta cells after 72 hrs by MTT assay	[PMID: 18930407]
HUVEC	IC₅₀	5.39 μM Compound: 19	Antiproliferative activity against HUVEC after 48 hrs by MTT assay Antiproliferative activity against HUVEC after 48 hrs by MTT assay	[PMID: 25350923]
Jurkat E6.1	IC₅₀	0.92 μM Compound: JS-K	Anticancer activity against human Jurkat E6-1 cells assessed as inhibition of cell growth after 72 hrs by MTT or MTS assay Anticancer activity against human Jurkat E6-1 cells assessed as inhibition of cell growth after 72 hrs by MTT or MTS assay	[PMID: 32156499]
L02	IC₅₀	1.99 μM Compound: 6; JS-K	Cytotoxicity against human HL7702 cells assessed as reduction in cell viability after 96 hrs by Celltiter-Glo assay Cytotoxicity against human HL7702 cells assessed as reduction in cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]
MCF-10A	IC₅₀	4.91 μM Compound: JS-K	Antiproliferative activity against human MCF10A cells after 48 hrs by MTT assay relative to control Antiproliferative activity against human MCF10A cells after 48 hrs by MTT assay relative to control	[PMID: 29220794]
MCF7	IC₅₀	993 nM Compound: 1; JS-K	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 29420887]
MDA-MB-231	IC₅₀	2.9 μM Compound: 6; JS-K	Antiproliferative activity against human MDA-MB-231 cells harboring KRAS G13D/BRAF G464V mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay Antiproliferative activity against human MDA-MB-231 cells harboring KRAS G13D/BRAF G464V mutant assessed as inhibition of cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]
MDA-MB-231	IC₅₀	5.75 μM Compound: JS-K	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay relative to control Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay relative to control	[PMID: 29220794]
MDA-MB-231	IC₅₀	7.93 μM Compound: JS-K	Anticancer activity against human MDA-MB-231 cells assessed as inhibition of cell growth after 72 hrs by MTT or MTS assay Anticancer activity against human MDA-MB-231 cells assessed as inhibition of cell growth after 72 hrs by MTT or MTS assay	[PMID: 32156499]
NCI-H1373	IC₅₀	0.86 μM Compound: 1, JS-K	Antiproliferative activity against human NCI-H1373 cells after 72 hrs by MTT assay Antiproliferative activity against human NCI-H1373 cells after 72 hrs by MTT assay	[PMID: 22003962]
NCI-H1703	IC₅₀	0.57 μM Compound: 1, JS-K	Antiproliferative activity against human NCI-H1703 cells after 72 hrs by MTT assay Antiproliferative activity against human NCI-H1703 cells after 72 hrs by MTT assay	[PMID: 22003962]
NCI-H1703	IC₅₀	1.08 μM Compound: 1, Js-K	Antiproliferative activity against human NCI-H1703 cells after 48 hrs by MTT assay Antiproliferative activity against human NCI-H1703 cells after 48 hrs by MTT assay	[PMID: 22480849]
NCI-H1944	IC₅₀	> 10 μM Compound: 1, JS-K	Antiproliferative activity against human NCI-H1944 cells after 72 hrs by MTT assay Antiproliferative activity against human NCI-H1944 cells after 72 hrs by MTT assay	[PMID: 22003962]
NCI-H2122	IC₅₀	3.17 μM Compound: 1, JS-K	Antiproliferative activity against human NCI-H2122 cells after 72 hrs by MTT assay Antiproliferative activity against human NCI-H2122 cells after 72 hrs by MTT assay	[PMID: 22003962]
NCI-H441	IC₅₀	0.94 μM Compound: 1, JS-K	Antiproliferative activity against human NCI-H441 cells after 72 hrs by MTT assay Antiproliferative activity against human NCI-H441 cells after 72 hrs by MTT assay	[PMID: 22003962]
NCI-H441	IC₅₀	1 μM Compound: JS-K	Antiproliferative activity against human H441 cells after 72 hrs by MTT assay Antiproliferative activity against human H441 cells after 72 hrs by MTT assay	[PMID: 18930407]
NCI-H838	IC₅₀	4.3 μM Compound: JS-K	Antiproliferative activity against human H838 cells after 72 hrs by MTT assay Antiproliferative activity against human H838 cells after 72 hrs by MTT assay	[PMID: 18930407]
OVCAR-3	IC₅₀	0.8 μM Compound: JS-K	Antiproliferative activity against human OVCAR-3 cells after 72 hrs by MTT assay Antiproliferative activity against human OVCAR-3 cells after 72 hrs by MTT assay	[PMID: 18930407]
OVCAR-5	IC₅₀	1 μM Compound: JS-K	Antiproliferative activity against human OVCAR5 cells after 72 hrs by MTT assay Antiproliferative activity against human OVCAR5 cells after 72 hrs by MTT assay	[PMID: 18930407]
PC-3	IC₅₀	1.1 μM Compound: JS-K	Antiproliferative activity against human PC-3 cells after 72 hrs by MTT assay Antiproliferative activity against human PC-3 cells after 72 hrs by MTT assay	[PMID: 18930407]
SMMC-7721	IC₅₀	7.31 μM Compound: JS-K	Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay Cytotoxicity against human SMMC7721 cells after 72 hrs by MTT assay	[PMID: 23617697]
SW-620	IC₅₀	5.16 μM Compound: JS-K	Cytotoxicity against human SW620 cells assessed as inhibition of cell proliferation after 48 hrs by MTT assay Cytotoxicity against human SW620 cells assessed as inhibition of cell proliferation after 48 hrs by MTT assay	10.1039/C5MD00158G
TK-10	IC₅₀	0.5 μM Compound: JS-K	Antiproliferative activity against human TK-10 cells after 72 hrs by MTT assay Antiproliferative activity against human TK-10 cells after 72 hrs by MTT assay	[PMID: 18930407]
U2OS	IC₅₀	6.27 μM Compound: JS-K	Antiproliferative activity against human U2OS cells after 48 hrs by MTT assay relative to control Antiproliferative activity against human U2OS cells after 48 hrs by MTT assay relative to control	[PMID: 29220794]
U-937	IC₅₀	0.3 μM Compound: JS-K	Antiproliferative activity against human U937 cells after 72 hrs by MTT assay Antiproliferative activity against human U937 cells after 72 hrs by MTT assay	[PMID: 18930407]
U-937	IC₅₀	0.3 μM Compound: JS-K	Antiproliferative activity against human U937 cells after 24 hrs Antiproliferative activity against human U937 cells after 24 hrs	[PMID: 19364650]
Vero	IC₅₀	1.16 μM Compound: 6; JS-K	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 96 hrs by Celltiter-Glo assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 96 hrs by Celltiter-Glo assay	[PMID: 32305784]

Molecular Weight

384.30

Formula

C₁₃H₁₆N₆O₈

CAS No.

205432-12-8

Appearance

Solid

Color

White to yellow

SMILES

O=C(N1CCN(/[N+]([O-])=N/OC2=CC=C([N+]([O-])=O)C=C2[N+]([O-])=O)CC1)OCC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 36 mg/mL (93.68 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6021 mL	13.0107 mL	26.0213 mL
5 mM	0.5204 mL	2.6021 mL	5.2043 mL
10 mM	0.2602 mL	1.3011 mL	2.6021 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.59%

Select Batch:

Data Sheet (267 KB) SDS (251 KB)

COA (246 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Shami PJ, et al. JS-K, a glutathione/glutathione S-transferase-activated nitric oxide donor of the diazeniumdiolate class with potent antineoplastic activity. Mol Cancer Ther. 2003 Apr;2(4):409-17. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6021 mL	13.0107 mL	26.0213 mL	65.0533 mL
	5 mM	0.5204 mL	2.6021 mL	5.2043 mL	13.0107 mL
	10 mM	0.2602 mL	1.3011 mL	2.6021 mL	6.5053 mL
	15 mM	0.1735 mL	0.8674 mL	1.7348 mL	4.3369 mL
	20 mM	0.1301 mL	0.6505 mL	1.3011 mL	3.2527 mL
	25 mM	0.1041 mL	0.5204 mL	1.0409 mL	2.6021 mL
	30 mM	0.0867 mL	0.4337 mL	0.8674 mL	2.1684 mL
	40 mM	0.0651 mL	0.3253 mL	0.6505 mL	1.6263 mL
	50 mM	0.0520 mL	0.2602 mL	0.5204 mL	1.3011 mL
	60 mM	0.0434 mL	0.2168 mL	0.4337 mL	1.0842 mL
	80 mM	0.0325 mL	0.1626 mL	0.3253 mL	0.8132 mL

JS-K Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JS-K205432-12-8NO SynthaseApoptosisNitric oxide synthasesNOSInhibitorinhibitorinhibit

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JS-K

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Complete Stock Solution Preparation Table

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