2. Stem Cell/Wnt
  3. Wnt
  4. JW67

JW67 inhibits the canonical Wnt signaling with an IC50 of 1.17μM. JW67 affects the multiprotein complex consisting of β-catenin/GSK-3β/AXIN/APC/CK1 that rapidly reduces active β-catenin with a subsequent downregulation of Wnt target genes. JW67 also inhibits colorectal cancer cell growth.

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JW67 Chemical Structure

JW67 Chemical Structure

CAS No. : 442644-28-2

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10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

JW67 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE JW67

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

JW67 inhibits the canonical Wnt signaling with an IC50 of 1.17μM[1]. JW67 affects the multiprotein complex consisting of β-catenin/GSK-3β/AXIN/APC/CK1 that rapidly reduces active β-catenin with a subsequent downregulation of Wnt target genes. JW67 also inhibits colorectal cancer cell growth[1].

IC50 & Target

IC50: 1.17μM (Wnt signaling)[1]
In Vitro

JW67 (1 μM; 24 hours) increases concentration of AXIN2 and decreases the active form of β-catenin[1].
JW67 affects the expression of Wnt target genes and reduces cell growth of colon cancer cell lines[1].
JW67 shows a concentrationdependent reduction in proliferation with a GI50 value of 7.8 μM[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

JW67 Related Antibodies

Western Blot Analysis[1]

Cell Line: SW480 cells
Concentration: 1 μM
Incubation Time: 24 hours
Result: Increased the AXIN2 expression.

RT-PCR[1]

Cell Line: SW480 cells
Concentration: 10 or 25μM
Incubation Time: 72 hours
Result: Reduced growth of SW480 CRC cells in vitro by inhibiting cell-cycle progression at the G1/S.
Molecular Weight

394.38

Formula

C21H18N2O6
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C2(OCC3(COC4(C5=CC=CC=C5NC4=O)OC3)CO2)C6=CC=CC=C6N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (126.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5356 mL 12.6781 mL 25.3563 mL
5 mM 0.5071 mL 2.5356 mL 5.0713 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

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C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (6.34 mM); Suspended solution; Need ultrasonic

    This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.34 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5356 mL 12.6781 mL 25.3563 mL 63.3906 mL
5 mM 0.5071 mL 2.5356 mL 5.0713 mL 12.6781 mL
10 mM 0.2536 mL 1.2678 mL 2.5356 mL 6.3391 mL
15 mM 0.1690 mL 0.8452 mL 1.6904 mL 4.2260 mL
20 mM 0.1268 mL 0.6339 mL 1.2678 mL 3.1695 mL
25 mM 0.1014 mL 0.5071 mL 1.0143 mL 2.5356 mL
30 mM 0.0845 mL 0.4226 mL 0.8452 mL 2.1130 mL
40 mM 0.0634 mL 0.3170 mL 0.6339 mL 1.5848 mL
50 mM 0.0507 mL 0.2536 mL 0.5071 mL 1.2678 mL
60 mM 0.0423 mL 0.2113 mL 0.4226 mL 1.0565 mL
80 mM 0.0317 mL 0.1585 mL 0.3170 mL 0.7924 mL
100 mM 0.0254 mL 0.1268 mL 0.2536 mL 0.6339 mL
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JW67 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JW67442644-28-2JW 67JW-67Wntβ-cateninGSK-3bAXINAPCCK1multiproteincomplexInhibitorinhibitorinhibit

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