L48H37

Name: L48H37
Brand: MedChemExpress
SKU: HY-126154
Rating: 4.5 (1 reviews)

Cat. No.: HY-126154 Purity: 97.05%: FAQs
Data Sheet SDS COA Handling Instructions

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L48H37 is an analog of Curcumin (HY-N0005) with improved chemical stability. L48H37 is a potent and specific myeloid differentiation protein 2 (MD2) inhibitor and inhibits the interaction and signaling transduction of LPS-TLR4/MD2. L48H37 is used for the research of sepsis or lung injury treatment.

For research use only. We do not sell to patients.

L48H37 Chemical Structure

CAS No. : 343307-76-6

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
5 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 380	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 620	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target^[1]

TLR4

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	4.2 μM Compound: 7B	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
HEp-2	IC₅₀	16.59 μM Compound: 569; CA15	Antiproliferative activity against human HEp-2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HEp-2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 32172081]
L02	IC₅₀	12.8 μM Compound: 7B	Cytotoxicity against human HL7702 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HL7702 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
L02	IC₅₀	46.79 μM Compound: 569; CA15	Antiproliferative activity against human HL7702 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HL7702 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 32172081]
NCI-H1650	IC₅₀	8.2 μM Compound: 7B	Cytotoxicity against human NCI-H1650 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human NCI-H1650 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
NCI-H1975	IC₅₀	6.5 μM Compound: 7B	Cytotoxicity against human NCI-H1975 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human NCI-H1975 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]
NCI-H460	IC₅₀	4.9 μM Compound: 7B	Cytotoxicity against human H460 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human H460 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29655083]

In Vitro

L48H37 inhibits LPS-induced inflammation, particularly TNF-α and IL-6 production and gene expression in mouse macrophages^[1].
L48H37 (0-20 µM; 24 hours) decreases the viability of A549 and H460 cells with IC₅₀ values of 5.3 µM and 2.3 µM, respectively, which is more effective compared to curcumin in lung cancer cells. It shows a low cytotoxicity on normal human lung epithelial cells (BEAS-2B) with IC₅₀ of 21 μM^[2].
L48H37 (1, 2, or 4 µM; 16 hours) dose‐dependently inhibited the expression of p‐Cdc2 and Cdc2, and increases the expression of p53. It also shows increased levels of cleaved poly (ADP‐ribosyl) polymerase (PARP) and reduced levels of anti‐apoptotic protein Bcl‐2 in H460 and A549 cells^[2].
L48H37 (4 µM; 16 hours) rapidly induces intracellular ROS levels dose-dependently as detected by increased DCF levels in H460 and A549 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	A549 and H460 cells; BEAS-2B cells
Concentration:	0.625, 1.25, 2.5, 5, 7.5, 10, and 20 µM
Incubation Time:	24 hours
Result:	Inhibited lung cancer cells growth in a concentration-dependent manner.

Western Blot Analysis^[2]

Cell Line:	A549 and H460 cells
Concentration:	0.625, 1.25, 2.5, 5, 7.5, 10, and 20 µM
Incubation Time:	24 hours
Result:	Decreased p‐Cdc2, Cdc2, and Bcl‐2 expression in 2 lung cancer cells.

In Vivo

L48H37 (intraperitoneal injection; 5 mg or 10 mg/kg; once daily; 11‐day ) inhibits H460 xenograft tumor growth and exhibits anti‐tumor activity in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	5‐week‐old athymic BALB/cA nu/nu female mice (18‐22 g)^[2]
Dosage:	5 mg or 10 mg/kg
Administration:	Intraperitoneal injection; once daily; 11‐day
Result:	Reduced tumor wet weights as compared to vehicle control. Decreased the levels of p‐STAT3, and increased the levels of p‐EIF2α and ATF4 in vivo. Exhibited no significant structural changes in mice.

Molecular Weight

483.55

Formula

C₂₇H₃₃NO₇

CAS No.

343307-76-6

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1/C(CN(C/C1=C\C2=CC(OC)=C(C(OC)=C2)OC)CC)=C/C3=CC(OC)=C(C(OC)=C3)OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (103.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0680 mL	10.3402 mL	20.6804 mL
5 mM	0.4136 mL	2.0680 mL	4.1361 mL
10 mM	0.2068 mL	1.0340 mL	2.0680 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 97.05%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (247 KB) HNMR (249 KB) LCMS (275 KB)

Handling Instructions (2659 KB)

References

[1]. Yi Wang, et al. Curcumin Analog L48H37 Prevents Lipopolysaccharide-Induced TLR4 Signaling Pathway Activation and Sepsis via Targeting MD2. J Pharmacol Exp Ther. 2015 Jun;353(3):539-50 [Content Brief]

[2]. Chen Feng, et al. Curcumin analog L48H37 induces apoptosis through ROS-mediated endoplasmic reticulum stress and STAT3 pathways in human lung cancer cells. Mol Carcinog. 2017 Jul;56(7):1765-1777. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0680 mL	10.3402 mL	20.6804 mL	51.7010 mL
	5 mM	0.4136 mL	2.0680 mL	4.1361 mL	10.3402 mL
	10 mM	0.2068 mL	1.0340 mL	2.0680 mL	5.1701 mL
	15 mM	0.1379 mL	0.6893 mL	1.3787 mL	3.4467 mL
	20 mM	0.1034 mL	0.5170 mL	1.0340 mL	2.5850 mL
	25 mM	0.0827 mL	0.4136 mL	0.8272 mL	2.0680 mL
	30 mM	0.0689 mL	0.3447 mL	0.6893 mL	1.7234 mL
	40 mM	0.0517 mL	0.2585 mL	0.5170 mL	1.2925 mL
	50 mM	0.0414 mL	0.2068 mL	0.4136 mL	1.0340 mL
	60 mM	0.0345 mL	0.1723 mL	0.3447 mL	0.8617 mL
	80 mM	0.0259 mL	0.1293 mL	0.2585 mL	0.6463 mL
	100 mM	0.0207 mL	0.1034 mL	0.2068 mL	0.5170 mL

L48H37 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

L48H37343307-76-6Toll-like Receptor (TLR)InflammationAsthmalung injurySepsisTLR4/MD2IL-6TNF-αCurcuminInhibitorinhibitorinhibit

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L48H37

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