MLS-573151 (Synonyms: MLS000573151)

Name: MLS-573151
Brand: MedChemExpress
SKU: HY-113849
Rating: 4.5 (1 reviews)

Cat. No.: HY-113849 Purity: 99.74%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

MLS-573151 (MLS000573151) is a selective GTPase Cdc42 inhibitor with an EC₅₀ of 2 μM. MLS-573151 is inactive against other GTPases family members, such as Rab2, Rab7, H-Ras, Rac1, Rac 2 and RhoA wild-type. MLS-573151 acts by blocking the binding of GTP to Cdc42.

For research use only. We do not sell to patients.

MLS-573151 Chemical Structure

CAS No. : 10179-57-4

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 110	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 110	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 100	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 950	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE MLS-573151

•Sci Adv. 2023 May 24;9(21):eadg1778. [Abstract]

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
BHK-21	CC₅₀	15 μM Compound: 4a	Cytotoxicity against golden hamster BHK21 cells assessed as decrease in cell viability after 72 hrs by MTT assay Cytotoxicity against golden hamster BHK21 cells assessed as decrease in cell viability after 72 hrs by MTT assay	[PMID: 29028528]
MT4	CC₅₀	42 μM Compound: 4a	Cytotoxicity against human MT4 cells assessed as decrease in cell viability after 96 hrs by MTT assay Cytotoxicity against human MT4 cells assessed as decrease in cell viability after 96 hrs by MTT assay	[PMID: 29028528]
Vero 76	CC₅₀	22 μM Compound: 4a	Cytotoxicity against African green monkey Vero 76 cells assessed as decrease in cell viability after 48 to 96 hrs by MTT assay Cytotoxicity against African green monkey Vero 76 cells assessed as decrease in cell viability after 48 to 96 hrs by MTT assay	[PMID: 29028528]

In Vitro

The fluorescence intensities of phagocytosed beads or bacteria in hemocytes, taken as a measure of phagocytosis efficiency, were markedly reduced in granulocytes treated with MLS-573151 (50 μM; for 15 min) compared to that in the control group. MLS-573151 could effectively inhibit the phagocytic ability of granulocytes^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

377.46

Formula

C₂₁H₁₉N₃O₂S

CAS No.

10179-57-4

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC=C(N2N=C(C3=CC=CC=C3)CC2C4=CC=CC=C4)C=C1)(N)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (264.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6493 mL	13.2464 mL	26.4929 mL
5 mM	0.5299 mL	2.6493 mL	5.2986 mL
10 mM	0.2649 mL	1.3246 mL	2.6493 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.74%

Select Batch:

Data Sheet (276 KB) SDS (393 KB)

COA (253 KB) HNMR (280 KB) LCMS (98 KB)

Handling Instructions (2659 KB)

References

[1]. Zurab Surviladze, et al. Identification of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay. J Biomol Screen. 2010 Jan;15(1):10-20. [Content Brief]

[2]. Fan Mao, et al. Transcriptomic Evidence Reveals the Molecular Basis for Functional Differentiation of Hemocytes in a Marine Invertebrate, Crassostrea gigas. Front Immunol. 2020 May 27;11:911. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6493 mL	13.2464 mL	26.4929 mL	66.2322 mL
	5 mM	0.5299 mL	2.6493 mL	5.2986 mL	13.2464 mL
	10 mM	0.2649 mL	1.3246 mL	2.6493 mL	6.6232 mL
	15 mM	0.1766 mL	0.8831 mL	1.7662 mL	4.4155 mL
	20 mM	0.1325 mL	0.6623 mL	1.3246 mL	3.3116 mL
	25 mM	0.1060 mL	0.5299 mL	1.0597 mL	2.6493 mL
	30 mM	0.0883 mL	0.4415 mL	0.8831 mL	2.2077 mL
	40 mM	0.0662 mL	0.3312 mL	0.6623 mL	1.6558 mL
	50 mM	0.0530 mL	0.2649 mL	0.5299 mL	1.3246 mL
	60 mM	0.0442 mL	0.2208 mL	0.4415 mL	1.1039 mL
	80 mM	0.0331 mL	0.1656 mL	0.3312 mL	0.8279 mL
	100 mM	0.0265 mL	0.1325 mL	0.2649 mL	0.6623 mL

MLS-573151 Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MLS-57315110179-57-4MLS000573151MLS573151MLS 573151MLS 000573151MLS-000573151RasGTPaseCdc42granulocytesInhibitorinhibitorinhibit

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MLS-573151 (Synonyms: MLS000573151)

Customer Review

MLS-573151 Related Antibodies

1 Publications Citing Use of MCE MLS-573151

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Ras Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

MLS-573151 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

MLS-573151 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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