MLT-231

Name: MLT-231
Brand: MedChemExpress
SKU: HY-131902
Rating: 4.5 (1 reviews)

Cat. No.: HY-131902 Purity: 99.55%: FAQs
Data Sheet SDS COA Handling Instructions

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MLT-231 is a potent, highly selective allosteric MALT1 Inhibitor with an IC₅₀ of 9 nM. MLT-231 specifically prevents endogenous BCL10 cleavage with IC₅₀ of 160 nM. MLT-231 shows antitumor activity in an ABC-DLBCL type xenograft model in mouse.

For research use only. We do not sell to patients.

MLT-231 Chemical Structure

CAS No. : 2682102-10-7

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 638	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 638	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 580	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 950	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1900	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 3000	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 4800	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
BJAB	IC₅₀	2 μM Compound: 27; MLT-231	Antiproliferation activity against human BJAB cells assessed as reduction in cell viability incubated for 14 days by resazurin dye-based fluorescence analysis Antiproliferation activity against human BJAB cells assessed as reduction in cell viability incubated for 14 days by resazurin dye-based fluorescence analysis	[PMID: 33252239]
BMDM	IC₅₀	> 10 μM Compound: 27; MLT-231	Cytotoxicity against mouse Bone marrow-derived macrophage cells assessed as reduction in cell viability by [3H]-thymidine incorporation assay Cytotoxicity against mouse Bone marrow-derived macrophage cells assessed as reduction in cell viability by [3H]-thymidine incorporation assay	[PMID: 33252239]
OCI-Ly3	IC₅₀	> 10 μM Compound: 27; MLT-231	Antiproliferation activity against human OCI-LY3 cells assessed as reduction in cell viability incubated for 14 days by resazurin dye-based fluorescence analysis Antiproliferation activity against human OCI-LY3 cells assessed as reduction in cell viability incubated for 14 days by resazurin dye-based fluorescence analysis	[PMID: 33252239]
PBMC	IC₅₀	0.19 μM Compound: 27; MLT-231	Inhibition of mixed lymphocyte reaction in human PBMC cells assessed as reduction in [3H]-thymidine incorporation measured after 5 days in presence of Il-7 by microbeta counting method Inhibition of mixed lymphocyte reaction in human PBMC cells assessed as reduction in [3H]-thymidine incorporation measured after 5 days in presence of Il-7 by microbeta counting method	[PMID: 33252239]

In Vitro

MLT-231 (19.5-10000 nM) inhibits the proliferation of OCI-Ly3 cells. MLT-231 (50-5000 nM; 24 hours) leads to accumulation of the uncleaved form of its substrates CYLD, BCL10, and RELB while expression of the NF-κB target gene IRF4 is suppressed^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	OCI-Ly3 cells
Concentration:	50-5000 nM
Incubation Time:	24 hours
Result:	NF-κB target gene IRF4 was suppressed.

In Vivo

MLT-231 (10-100 mg/kg; p.o.; bid schedule for 2 weeks) displays in vivo efficacy in the ABC-DLBCL xenograft model^[1].
MLT-231 (1 mg/kg; i.v.; BALB/c mice) treatment shows the CL, t_1/2, and V_ss are 11 mL/min/kg, 1.9 hours, and 1.5 L/kg, respectively^[1].
MLT-231 (1 mg/kg; i.v.; Sprague-Dawley rats) treatment shows the CL, t_1/2, and V_ss are 41 mL/min/kg, 3.2 hours, and 9.4 L/kg, respectively^[1].
MLT-231 (3 mg/kg; p.o.; BALB/c mice) treatment shows the AUC_0-24, C_max and F are 3096 nM/h, 549 nM, and 99%, respectively^[1].
MLT-231 (3 mg/kg; p.o.; Sprague-Dawley rats) treatment shows the AUC_0-24, C_max and F are 547 nM/h, 46 nM, and 61%, respectively.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Scid-beige mice (OCI-Ly10 ABC-DLBCL type xenograft model)^[1]
Dosage:	10, 30, and 100 mg/kg
Administration:	P.o.; bid schedule for 2 weeks
Result:	Led to tumor stasis, while being well tolerated.

Molecular Weight

469.85

Formula

C₁₉H₁₉ClF₃N₇O₂

CAS No.

2682102-10-7

Appearance

Solid

Color

White to off-white

SMILES

O=C(NC1=C(N2[C@@H](C)COC[C@@H]2C)N3C(N=C1)=CC(Cl)=N3)NC4=CC=NC(C(F)(F)F)=C4

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 110 mg/mL (234.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1283 mL	10.6417 mL	21.2834 mL
5 mM	0.4257 mL	2.1283 mL	4.2567 mL
10 mM	0.2128 mL	1.0642 mL	2.1283 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 6 mg/mL (12.77 mM); Clear solution

This protocol yields a clear solution of ≥ 6 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (60.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5.5 mg/mL (11.71 mM); Clear solution

This protocol yields a clear solution of ≥ 5.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (55.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.55%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (248 KB) HNMR (445 KB) LCMS (110 KB)

Handling Instructions (2659 KB)

References

[1]. Pissot Soldermann C, et al. Discovery of Potent, Highly Selective, and In Vivo Efficacious, Allosteric MALT1 Inhibitors by Iterative Scaffold Morphing [published online ahead of print, 2020 Nov 30]. J Med Chem. 2020;10.1021/acs.jmedchem.0c01245. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1283 mL	10.6417 mL	21.2834 mL	53.2085 mL
	5 mM	0.4257 mL	2.1283 mL	4.2567 mL	10.6417 mL
	10 mM	0.2128 mL	1.0642 mL	2.1283 mL	5.3208 mL
	15 mM	0.1419 mL	0.7094 mL	1.4189 mL	3.5472 mL
	20 mM	0.1064 mL	0.5321 mL	1.0642 mL	2.6604 mL
	25 mM	0.0851 mL	0.4257 mL	0.8513 mL	2.1283 mL
	30 mM	0.0709 mL	0.3547 mL	0.7094 mL	1.7736 mL
	40 mM	0.0532 mL	0.2660 mL	0.5321 mL	1.3302 mL
	50 mM	0.0426 mL	0.2128 mL	0.4257 mL	1.0642 mL
	60 mM	0.0355 mL	0.1774 mL	0.3547 mL	0.8868 mL
	80 mM	0.0266 mL	0.1330 mL	0.2660 mL	0.6651 mL
	100 mM	0.0213 mL	0.1064 mL	0.2128 mL	0.5321 mL

MLT-231 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MLT-2312682102-10-7MLT231MLT 231MALT1mucosa associated lymphoid tissue lymphoma translocation gene 1primaryimmunecellsBlymphomaantitumorABC-DLBCLtypexenograftmodelInhibitorinhibitorinhibit

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MLT-231

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Complete Stock Solution Preparation Table

MLT-231 Related Classifications

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