2. Metabolic Enzyme/Protease
  3. Endogenous Metabolite
  4. Mono-(2-ethylhexyl) phthalate

Mono-(2-ethylhexyl) phthalate  (Synonyms: MEHP; Phthalic acid mono-2-ethylhexyl ester)

Cat. No.: HY-W018392 Purity: 97.08%
COA Handling Instructions

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Mono-(2-ethylhexyl) phthalate (MEHP) is a major bioactive metabolite of diethylhexyl phthalate (DEHP). Mono-(2-ethylhexyl) phthalate can promote fatty acid synthesis in hepatocytes by regulating the expression of relevant genes and proteins, contributing to non-alcoholic fatty liver disease (NAFLD).

For research use only. We do not sell to patients.

Mono-(2-ethylhexyl) phthalate Chemical Structure

Mono-(2-ethylhexyl) phthalate Chemical Structure

CAS No. : 4376-20-9

Size Price Stock Quantity
Solid or liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 41 In-stock
Solution
10 mM * 1 mL in DMSO USD 41 In-stock
Solid or liquid
50 mg USD 38 In-stock
100 mg USD 60 In-stock
200 mg   Get quote  
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Based on 7 publication(s) in Google Scholar

Other Forms of Mono-(2-ethylhexyl) phthalate:

Mono-(2-ethylhexyl) phthalate Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Mono-(2-ethylhexyl) phthalate (MEHP) is a major bioactive metabolite of diethylhexyl phthalate (DEHP). Mono-(2-ethylhexyl) phthalate can promote fatty acid synthesis in hepatocytes by regulating the expression of relevant genes and proteins, contributing to non-alcoholic fatty liver disease (NAFLD)[1].

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

Mono-(2-ethylhexyl) phthalate (0-100 μM, 48 h) can promote lipid accumulation in HepG2 cells[1].
Mono-(2-ethylhexyl) phthalate (0-100 μM, 24-48 h) increases the TG level in HepG2 cells in a dose-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mono-(2-ethylhexyl) phthalate Related Antibodies

Cell Viability Assay[1]

Cell Line: HepG2 cells
Concentration: 0-250 μM
Incubation Time: 24-72 h
Result: Decreased cell viability at 50-250 μM.

Western Blot Analysis[1]

Cell Line: HepG2 cells
Concentration: 0-100 μM
Incubation Time: 6-48 h
Result: Increased the levels of ACC1, FASN, and SCD proteins after (24 h).
Decreased the levels of ACC1, FASN, and SCD proteins (48 h).
Increased the levels of ChREBP (24-48 h).
Increased the expressions of five genes (SREBP-1c, ACC1, FASN, SCD and ChREBP) within a short period of time (6 h), and decreased with prolonged exposure (24 and 48 h).
In Vivo

Mono-(2-ethylhexyl) phthalate (0.1-1 mL/kg, p.o., daily, 3 days) causes fetal malformations and reduces live fetuses in pregnant mice[2].
Mono-(2-ethylhexyl) phthalate (25-50 mg/kg, i.p., once time) induces a significantly high incidence of somatic mutations in the coat hair of offspring of mice[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

278.34

Formula

C16H22O4
CAS No.
Appearance

<25°C Solid,>27°C Liquid

Color

Colorless to light yellow

SMILES

O=C(O)C1=CC=CC=C1C(OCC(CC)CCCC)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 1 year
-20°C 6 months
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (359.27 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.5927 mL 17.9636 mL 35.9273 mL
5 mM 0.7185 mL 3.5927 mL 7.1855 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (8.98 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 4.29 mg/mL (15.41 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 97.08%

COA (246 KB) HNMR (285 KB) LCMS (104 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.5927 mL 17.9636 mL 35.9273 mL 89.8182 mL
5 mM 0.7185 mL 3.5927 mL 7.1855 mL 17.9636 mL
10 mM 0.3593 mL 1.7964 mL 3.5927 mL 8.9818 mL
15 mM 0.2395 mL 1.1976 mL 2.3952 mL 5.9879 mL
20 mM 0.1796 mL 0.8982 mL 1.7964 mL 4.4909 mL
25 mM 0.1437 mL 0.7185 mL 1.4371 mL 3.5927 mL
30 mM 0.1198 mL 0.5988 mL 1.1976 mL 2.9939 mL
40 mM 0.0898 mL 0.4491 mL 0.8982 mL 2.2455 mL
50 mM 0.0719 mL 0.3593 mL 0.7185 mL 1.7964 mL
60 mM 0.0599 mL 0.2994 mL 0.5988 mL 1.4970 mL
80 mM 0.0449 mL 0.2245 mL 0.4491 mL 1.1227 mL
100 mM 0.0359 mL 0.1796 mL 0.3593 mL 0.8982 mL
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Mono-(2-ethylhexyl) phthalate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Mono-(2-ethylhexyl) phthalate4376-20-9MEHP Phthalic acid mono-2-ethylhexyl esterEndogenous MetaboliteDEHPNAFLDfatty acidhepatocytesInhibitorinhibitorinhibit

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