N-Benzyllinolenamide

Name: N-Benzyllinolenamide
Brand: MedChemExpress
SKU: HY-N3033
Rating: 4.5 (1 reviews)

Cat. No.: HY-N3033 Purity: 99.70%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

N-Benzyllinolenamide is a natural macamide isolated from Lepidium meyenii, acts as an inhibitor of fatty acid amide hydrolase (FAAH) with an IC₅₀ of 41.8 μM.

For research use only. We do not sell to patients.

N-Benzyllinolenamide Chemical Structure

CAS No. : 883715-18-2

Size	Price	Stock	Quantity
5 mg	USD 230	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE N-Benzyllinolenamide

•Cell Death Differ. 2022 Sep 14. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

N-Benzyllinolenamide is a natural macamide isolated from Lepidium meyenii, acts as an inhibitor of fatty acid amide hydrolase (FAAH) with an IC₅₀ of 41.8 μM^[1].

IC₅₀ & Target

IC50: 41.8 μM (FAAH)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
U-937	IC₅₀	11.48 μM Compound: 6	Inhibition of FAAH-mediated [ethanol-amine-1-3H]AEA hydrolysis in human U937 cells preincubated for 15 mins measured 15 mins post AEA addition by liquid scintillation counting Inhibition of FAAH-mediated [ethanol-amine-1-3H]AEA hydrolysis in human U937 cells preincubated for 15 mins measured 15 mins post AEA addition by liquid scintillation counting	[PMID: 24972328]
U-937	IC₅₀	84.36 μM Compound: 6	Inhibition of [ethanol-amine-1-3H]AEA uptake in human U937 cells preincubated for 15 mins measured 15 mins post AEA addition by liquid scintillation counting Inhibition of [ethanol-amine-1-3H]AEA uptake in human U937 cells preincubated for 15 mins measured 15 mins post AEA addition by liquid scintillation counting	[PMID: 24972328]

Molecular Weight

367.57

Formula

C₂₅H₃₇NO

CAS No.

883715-18-2

Appearance

Solid

Color

Off-white to light yellow

SMILES

CC/C=C\C/C=C\C/C=C\CCCCCCCC(NCC1=CC=CC=C1)=O

Structure Classification

Alkaloids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (272.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7206 mL	13.6029 mL	27.2057 mL
5 mM	0.5441 mL	2.7206 mL	5.4411 mL
10 mM	0.2721 mL	1.3603 mL	2.7206 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (6.80 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.80 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.70%

Select Batch:

Data Sheet (266 KB) SDS (394 KB)

COA (239 KB) HNMR (191 KB) RP-HPLC (256 KB)

Handling Instructions (2659 KB)

References

[1]. Wu H, et al. Macamides and their synthetic analogs: evaluation of in vitro FAAH inhibition. Bioorg Med Chem. 2013 Sep 1;21(17):5188-97. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7206 mL	13.6029 mL	27.2057 mL	68.0143 mL
	5 mM	0.5441 mL	2.7206 mL	5.4411 mL	13.6029 mL
	10 mM	0.2721 mL	1.3603 mL	2.7206 mL	6.8014 mL
	15 mM	0.1814 mL	0.9069 mL	1.8137 mL	4.5343 mL
	20 mM	0.1360 mL	0.6801 mL	1.3603 mL	3.4007 mL
	25 mM	0.1088 mL	0.5441 mL	1.0882 mL	2.7206 mL
	30 mM	0.0907 mL	0.4534 mL	0.9069 mL	2.2671 mL
	40 mM	0.0680 mL	0.3401 mL	0.6801 mL	1.7004 mL
	50 mM	0.0544 mL	0.2721 mL	0.5441 mL	1.3603 mL
	60 mM	0.0453 mL	0.2267 mL	0.4534 mL	1.1336 mL
	80 mM	0.0340 mL	0.1700 mL	0.3401 mL	0.8502 mL
	100 mM	0.0272 mL	0.1360 mL	0.2721 mL	0.6801 mL