N1,N8-Diacetylspermidine

Name: N1,N8-Diacetylspermidine
Brand: MedChemExpress
SKU: HY-129912
Rating: 4.5 (1 reviews)

Cat. No.: HY-129912 Purity: ≥99.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

N1,N8-Diacetylspermidine is a polyamines in the human urine. N1,N8-Diacetylspermidine is useful as prognostic indicators after treatment and during follow-up examination of cancer patients.

For research use only. We do not sell to patients.

N1,N8-Diacetylspermidine Chemical Structure

CAS No. : 82414-35-5

Size	Price	Stock	Quantity
5 mg	USD 245	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 425	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of N1,N8-Diacetylspermidine:

N1,N8-Diacetylspermidine hydrochloride Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

N1,N8-Diacetylspermidine is a polyamines in the human urine^[1]. N1,N8-Diacetylspermidine is useful as prognostic indicators after treatment and during follow-up examination of cancer patients^[2].

IC₅₀ & Target^[1]

Human Endogenous Metabolite

Molecular Weight

229.32

Formula

C₁₁H₂₃N₃O₂

CAS No.

82414-35-5

Appearance

Solid

Color

White to off-white

SMILES

CC(NCCCNCCCCNC(C)=O)=O

Structure Classification

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (436.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.3607 mL	21.8036 mL	43.6072 mL
5 mM	0.8721 mL	4.3607 mL	8.7214 mL
10 mM	0.4361 mL	2.1804 mL	4.3607 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.90 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (267 KB) SDS (251 KB)

COA (241 KB) HNMR (133 KB) RP-HPLC (405 KB) MS (151 KB)

Handling Instructions (2659 KB)

References

[1]. Umemori Y, et al. Evaluating the utility of N1,N12-diacetylspermine and N1,N8-diacetylspermidine in urine as tumormarkers for breast and colorectal cancers. Clin Chim Acta. 2010 Dec 14;411(23-24):1894-9. [Content Brief]

[2]. Hiramatsu K, et al. Diagnostic and prognostic usefulness of N1,N8-diacetylspermidine and N1,N12-diacetylsperminein urine as novel markers of malignancy. J Cancer Res Clin Oncol. 1997;123(10):539-45 [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.3607 mL	21.8036 mL	43.6072 mL	109.0180 mL
	5 mM	0.8721 mL	4.3607 mL	8.7214 mL	21.8036 mL
	10 mM	0.4361 mL	2.1804 mL	4.3607 mL	10.9018 mL
	15 mM	0.2907 mL	1.4536 mL	2.9071 mL	7.2679 mL
	20 mM	0.2180 mL	1.0902 mL	2.1804 mL	5.4509 mL
	25 mM	0.1744 mL	0.8721 mL	1.7443 mL	4.3607 mL
	30 mM	0.1454 mL	0.7268 mL	1.4536 mL	3.6339 mL
	40 mM	0.1090 mL	0.5451 mL	1.0902 mL	2.7254 mL
	50 mM	0.0872 mL	0.4361 mL	0.8721 mL	2.1804 mL
	60 mM	0.0727 mL	0.3634 mL	0.7268 mL	1.8170 mL
	80 mM	0.0545 mL	0.2725 mL	0.5451 mL	1.3627 mL
	100 mM	0.0436 mL	0.2180 mL	0.4361 mL	1.0902 mL