2. Apoptosis Autophagy Metabolic Enzyme/Protease
  3. Apoptosis Autophagy Glutathione S-transferase
  4. NBDHEX

NBDHEX is a potent glutathione S-transferase P1-1 (GSTP1-1) inhibitor. NBDHEX induces apoptosis of tumor cells. NBDHEX acts as an anticancer agent by inhibiting GSTs catalytic activity, avoiding inconvenience of the inhibitor extrusion from the cell by specific pumps and disrupting the interaction between the GSTP1-1 and key signaling effectors. NBDHEX can also act as late-phase autophagy inhibitor.

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NBDHEX Chemical Structure

NBDHEX Chemical Structure

CAS No. : 787634-60-0

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10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

NBDHEX Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE NBDHEX

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

NBDHEX is a potent glutathione S-transferase P1-1 (GSTP1-1) inhibitor. NBDHEX induces apoptosis of tumor cells. NBDHEX acts as an anticancer agent by inhibiting GSTs catalytic activity, avoiding inconvenience of the inhibitor extrusion from the cell by specific pumps and disrupting the interaction between the GSTP1-1 and key signaling effectors. NBDHEX can also act as late-phase autophagy inhibitor[1][2].

IC50 & Target

Glutathione S-transferase P1-1 (GSTP1-1)[1];
Apoptosis[1];
Autophagy[1]
Cellular Effect
Cell Line Type Value Description References
143B IC50
4.08 μM
Compound: NBDHEX
Antiproliferative activity against human 143B cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
Antiproliferative activity against human 143B cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
[PMID: 33529017]
A549 IC50
2.88 μM
Compound: NBDHEX
Antiproliferative activity against human A549 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
Antiproliferative activity against human A549 cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
[PMID: 33529017]
HOS IC50
1.24 μM
Compound: NBDHEX
Antiproliferative activity against human HOS cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
Antiproliferative activity against human HOS cells assessed as inhibition of cell proliferation incubated for 72 hrs by MTT assay
[PMID: 33529017]
HUVEC IC50
0.5 μM
Compound: NBDHEX
Cytotoxicity against HUVEC assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Cytotoxicity against HUVEC assessed as reduction in cell viability incubated for 72 hrs by MTT assay
[PMID: 33529017]
L02 IC50
1.56 μM
Compound: NBDHEX
Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay
[PMID: 33529017]
U2OS IC50
0.6 μM
Compound: 1, MC2754, NBDHEX
Cytotoxicity against human U2OS cells assessed as cell survival after 48 hrs SRB assay
Cytotoxicity against human U2OS cells assessed as cell survival after 48 hrs SRB assay
[PMID: 25462236]
In Vitro

NBDHEX (0.05-20 μM; 48 hours; H69 and H69AR cells) is cytotoxic toward cell lung cancer H69 and H69AR cells[2].
? NBDHEX (0-5 μM; 24 hours; H69AR cells) treatment results in a dose-dependent apoptosis in the H69AR cell line[2].
? NBDHEX (3 μM; 1-12 hours; H69AR cells) treatment increases the phosphorylation of JNK/c-Jun in H69AR cells in a time-dependent fashion[2].
? NBDHEX treatment shows a marked increase in phosphorylation of p38MAPK, and also increases GSSG content in a time-dependent manner in H69 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

NBDHEX Related Antibodies

Cell Viability Assay[2]

Cell Line: H69 and H69AR cells
Concentration: 0.05-20 μM
Incubation Time: 48 hours
Result: The dose-response profiles revealed a good cytotoxic activity both in sensitive H69 cell line (LC50 of 2.3 μM) and in its Adriamycin-resistant counterpart H69AR (LC50 of 4.5 μM).

Apoptosis Analysis[2]

Cell Line: H69AR cells
Concentration: 0 μM, 0.5 μM, 1 μM, 2 μM, 3 μM, 4 μM, 5 μM
Incubation Time: 24 hours
Result: Resulted in a dose-dependent apoptosis in the H69AR cell line.

Western Blot Analysis[2]

Cell Line: H69AR cells
Concentration: 3 μM
Incubation Time: 1 hour,3 hours, 6 hours, 12 hours
Result: Increased the phosphorylation of JNK/c-Jun in H69AR cells in a time-dependent fashion.
In Vivo

NBDHEX (0.8-80 mg/kg/day; oral administration; daily; for 15 days; SCID female mice) treatment results a statistically significant tumour inhibition (approximately 70%)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: SCID female mice (4-5 weeks) injected with Me501cells[3]
Dosage: 0.8 mg/kg/day, 8.0 mg/kg/day or 80 mg/kg/day
Administration: Oral administration; daily; for 15 days
Result: A statistically significant tumour inhibition (approximately 70%) was observed.
Molecular Weight

297.33

Formula

C12H15N3O4S
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

OCCCCCCSC1=CC=C([N+]([O-])=O)C2=NON=C21
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (420.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3633 mL 16.8163 mL 33.6327 mL
5 mM 0.6727 mL 3.3633 mL 6.7265 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (7.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (7.00 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3633 mL 16.8163 mL 33.6327 mL 84.0817 mL
5 mM 0.6727 mL 3.3633 mL 6.7265 mL 16.8163 mL
10 mM 0.3363 mL 1.6816 mL 3.3633 mL 8.4082 mL
15 mM 0.2242 mL 1.1211 mL 2.2422 mL 5.6054 mL
20 mM 0.1682 mL 0.8408 mL 1.6816 mL 4.2041 mL
25 mM 0.1345 mL 0.6727 mL 1.3453 mL 3.3633 mL
30 mM 0.1121 mL 0.5605 mL 1.1211 mL 2.8027 mL
40 mM 0.0841 mL 0.4204 mL 0.8408 mL 2.1020 mL
50 mM 0.0673 mL 0.3363 mL 0.6727 mL 1.6816 mL
60 mM 0.0561 mL 0.2803 mL 0.5605 mL 1.4014 mL
80 mM 0.0420 mL 0.2102 mL 0.4204 mL 1.0510 mL
100 mM 0.0336 mL 0.1682 mL 0.3363 mL 0.8408 mL
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NBDHEX Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

NBDHEX787634-60-0ApoptosisAutophagyGlutathione S-transferaseGlutathione transferasesGSTsautophagyanticancercatalyticextrusioncytotoxicJNKc-JunGSSGp38MAPKInhibitorinhibitorinhibit

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