OncoFAP

Name: OncoFAP
Brand: MedChemExpress
SKU: HY-145938
Rating: 4.5 (1 reviews)

Cat. No.: HY-145938 Purity: 99.77%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

OncoFAP is an ultra-high-affinity ligand of fibroblast activation protein (FAP) for targeting applications with pan-tumoral potential.

For research use only. We do not sell to patients.

OncoFAP Chemical Structure

CAS No. : 2639365-69-6

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 608	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 608	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 222	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 553	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 875	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1750	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2835	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 4550	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

OncoFAP is an ultra-high-affinity ligand of fibroblast activation protein (FAP) for targeting applications with pan-tumoral potential.

In Vitro

OncoFAP-Fluorescein (15 nM, 1 hour) can efficiently internalize in the HT-1080.hFAP tumor cell line^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

177Lu-OncoFAP-DOTAGA (50 nmol/kg - 1 μmol/kg, i.v., single dose) efficiently targets FAP-positive tumors in the SK-RC-52.hFAP xenograft model, with dose-dependent accumulation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Xenograft model of humanized FAP renal cancer (SK-RC-52.hFAP) in nude mice^[1]
Dosage:	50, 125, 250, 500 nmol/kg, and 1 μmol/kg (in the form of 177Lu-OncoFAP-DOTAGA)
Administration:	Intravenous injection (i.v.), single dose
Result:	Reached 32% of the injected dose per gram of tumor tissue (ID/g) within 10 minutes and maintained above 20% ID/g for 6 hours.

Molecular Weight

459.40

Formula

C₂₁H₁₉F₂N₅O₅

CAS No.

2639365-69-6

Appearance

Solid

Color

White to light yellow

SMILES

OC(CCC(NC1=CC=CC2=C1N=CC=C2C(NCC(N3[C@@H](CC(F)(C3)F)C#N)=O)=O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (217.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1768 mL	10.8838 mL	21.7675 mL
5 mM	0.4354 mL	2.1768 mL	4.3535 mL
10 mM	0.2177 mL	1.0884 mL	2.1768 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (5.44 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.77%

Select Batch:

Data Sheet (270 KB) SDS (251 KB)

COA (245 KB) HNMR (253 KB) CNMR (263 KB) RP-HPLC (265 KB) LCMS (139 KB)

Handling Instructions (2659 KB)

References

[1]. Millul J, et al. An ultra-high-affinity small organic ligand of fibroblast activation protein for tumor-targeting applications. Proc Natl Acad Sci U S A. 2021 Apr 20;118(16):e2101852118. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1768 mL	10.8838 mL	21.7675 mL	54.4188 mL
	5 mM	0.4354 mL	2.1768 mL	4.3535 mL	10.8838 mL
	10 mM	0.2177 mL	1.0884 mL	2.1768 mL	5.4419 mL
	15 mM	0.1451 mL	0.7256 mL	1.4512 mL	3.6279 mL
	20 mM	0.1088 mL	0.5442 mL	1.0884 mL	2.7209 mL
	25 mM	0.0871 mL	0.4354 mL	0.8707 mL	2.1768 mL
	30 mM	0.0726 mL	0.3628 mL	0.7256 mL	1.8140 mL
	40 mM	0.0544 mL	0.2721 mL	0.5442 mL	1.3605 mL
	50 mM	0.0435 mL	0.2177 mL	0.4354 mL	1.0884 mL
	60 mM	0.0363 mL	0.1814 mL	0.3628 mL	0.9070 mL
	80 mM	0.0272 mL	0.1360 mL	0.2721 mL	0.6802 mL
	100 mM	0.0218 mL	0.1088 mL	0.2177 mL	0.5442 mL

OncoFAP Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

OncoFAP2639365-69-6FAPFibroblast activation proteinAnti-cancerNude miceHT-1080.hFAP tumor cell lineInhibitorinhibitorinhibit

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OncoFAP

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OncoFAP Related Antibodies

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Complete Stock Solution Preparation Table

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