ONO-5334

Name: ONO-5334
Brand: MedChemExpress
SKU: HY-108044
Rating: 5.0 (2 reviews)

Cat. No.: HY-108044 Purity: 99.60%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

ONO-5334 is a potent, selective and orally active cathepsin K inhibitor with K_i values of 0.10 nM, 0.049 nM and 0.85 nM for human, rabbit and rat cathepsin K, respectively. ONO 5334 is an effective antiviral compound against SAR-COV-2 virus activity with an EC₅₀ value of 500 nM. ONO-5334 has the potential for the study of osteoporosis and COVID-19 disease.

For research use only. We do not sell to patients.

ONO-5334 Chemical Structure

CAS No. : 868273-90-9

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 550	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 550	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 800	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1760	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2640	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3960	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE ONO-5334

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•Related Screening Libraries:

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View All Cathepsin Isoform Specific Products:

View All Isoforms

Cathepsin B Cathepsin C Cathepsin D Cathepsin E Cathepsin K Cathepsin L Cathepsin S

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

cathepsin K

Cellular Effect

Cell Line	Type	Value	Description	References
Vero C1008	EC₅₀	0.5 μM Compound: ONO 5334	Determination of antiviral efficacy in high-content imaging assay in Vero E6 cells infected with SARS-CoV-2 (USA-WA1/2020 isolate) at MOI 0.75 after 24 hrs Determination of antiviral efficacy in high-content imaging assay in Vero E6 cells infected with SARS-CoV-2 (USA-WA1/2020 isolate) at MOI 0.75 after 24 hrs	[PMID: 32511357]

In Vitro

ONO-5334 has inhibitory effects on human cathepsin S, human cathepsin L, human cathepsin B, porcine calpain Ι and porcine calpain II with K_i values of 0.83 nM, 1.7 nM, 32 nM, 82 nM and 69 nM, respectively^[1].
ONO-5334 (0.1-1 μM; 24 hours) suppresses human osteoclast-mediated bone resorption. It potently reduces osteoclast-mediated release of CTX from bone slices as a dose dependent manner^[1].
ONO-5334 (0-10 μM; pre-treated for 16 h) inhibits antiviral activities in a discernable dose-dependent manner in Vero E6 cells by designed to capture multicycle replication, exhibiting an EC₅₀ value of 0.5 μM^[2]/

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	Vero E6 cells
Concentration:	0.001 μM, 0.003 μM, 0.1 μM, 0.3 μM, 1 μM, 2.5 μM
Incubation Time:	Pre-treated for 16 h and then cultured for 24 hours
Result:	Inhibited SARS-COV-2 virus replication in a dose-dependent manner.

In Vivo

ONO-5334 (oral administration; 0.12-15 mg/kg; single dose) can dose-dependently reduce PTHrP-induced increase in plasma calcium with significant effect (86% reduction) at 15 mg/kg. It also reduces PTHrP-induced increase in plasma CTX level in TPTX rats by 90% at 15 mg/kg^[1].
ONO-5334 (oral administration; 0.3-30 mg/kg; 7 consecutive days) at 3 mg/kg or 30 mg/kg significantly decreases CTX (a bone resorption marker) concentration. On day 7, the reduction in serum CTX concentration by ONO-5334 at 3 mg/kg and 30 mg/kg was 62% and 79%, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Monkey^[1]
Dosage:	0.3 mg/kg; 3 mg/kg
Administration:	Oral administration; 7 consecutive days
Result:	Reduced bone resorption markers but not bone formation markers in normal monkeys.

Clinical Trial

Molecular Weight

438.58

Formula

C₂₁H₃₄N₄O₄S

CAS No.

868273-90-9

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C(C(N/N=C(SC[C@H]1C)/N1C)=O)[C@H](C2CCOCC2)NC(C3CCCCCC3)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (114.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2801 mL	11.4004 mL	22.8009 mL
5 mM	0.4560 mL	2.2801 mL	4.5602 mL
10 mM	0.2280 mL	1.1400 mL	2.2801 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (249 KB) LCMS (113 KB) Elemental Analysis Report (106 KB)

Handling Instructions (2659 KB)

References

[1]. Ochi Y, et al. Effects of ONO-5334, a novel orally-active inhibitor of cathepsin K, on bone metabolism. Bone. 2011 Dec;49(6):1351-6. [Content Brief]

[2]. Laura Riva, et al.A Large-scale Drug Repositioning Survey for SARS-CoV-2 Antivirals. bioRxiv. 2020 Apr 17;2020.04.16.044016. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2801 mL	11.4004 mL	22.8009 mL	57.0021 mL
	5 mM	0.4560 mL	2.2801 mL	4.5602 mL	11.4004 mL
	10 mM	0.2280 mL	1.1400 mL	2.2801 mL	5.7002 mL
	15 mM	0.1520 mL	0.7600 mL	1.5201 mL	3.8001 mL
	20 mM	0.1140 mL	0.5700 mL	1.1400 mL	2.8501 mL
	25 mM	0.0912 mL	0.4560 mL	0.9120 mL	2.2801 mL
	30 mM	0.0760 mL	0.3800 mL	0.7600 mL	1.9001 mL
	40 mM	0.0570 mL	0.2850 mL	0.5700 mL	1.4251 mL
	50 mM	0.0456 mL	0.2280 mL	0.4560 mL	1.1400 mL
	60 mM	0.0380 mL	0.1900 mL	0.3800 mL	0.9500 mL
	80 mM	0.0285 mL	0.1425 mL	0.2850 mL	0.7125 mL
	100 mM	0.0228 mL	0.1140 mL	0.2280 mL	0.5700 mL

ONO-5334 Related Classifications

Metabolic Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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ONO-5334

Customer Review

ONO-5334 Related Antibodies

2 Publications Citing Use of MCE ONO-5334

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Cathepsin Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

ONO-5334 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

ONO-5334 Related Classifications

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