Phenazine-1-carboxylic acid

Name: Phenazine-1-carboxylic acid
Brand: MedChemExpress
SKU: HY-33037
Rating: 4.5 (1 reviews)

Cat. No.: HY-33037 Purity: 99.88%: FAQs
Data Sheet SDS COA Handling Instructions

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Phenazine-1-carboxylic acid is an antifungal agent. Additionally, Phenazine-1-carboxylic acid exhibits anticancer activity by inducing apoptosis in cancer cells through the regulation of ROS generation. Phenazine-1-carboxylic acid can upregulate the expression of IL-8 and ICAM-1 while inhibiting the release of RANTES and MCP-1, demonstrating its potential immunomodulatory effects. Phenazine-1-carboxylic acid holds significant research value in the areas of anti-infection, anticancer, and immune response modulation.

For research use only. We do not sell to patients.

Phenazine-1-carboxylic acid Chemical Structure

CAS No. : 2538-68-3

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 99	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 99	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 180	In-stock	Estimated Time of Arrival: December 31
1 g	USD 450	In-stock	Estimated Time of Arrival: December 31
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10 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	15.6 μM Compound: 4, Tubermycin B	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 5 days by hemocytometry Cytotoxicity against human HCT116 cells assessed as growth inhibition after 5 days by hemocytometry	[PMID: 23402329]

In Vitro

Phenazine-1-carboxylic acid (10-100 µM, 24 and 48 hours) can effectively inhibit the proliferation of DU145 cells in a time- and dose-dependent manner^[2].
Phenazine-1-carboxylic acid (50 µM, 24 hours) can induce apoptosis in DU145 cells through ROS generation and mitochondrial apoptosis pathways^[2].
Phenazine-1-carboxylic acid (50 µM, 36 hours) can upregulate IL-8 and ICAM-1 expression, inhibit RANTES and MCP-1 release, and has potential immunomodulatory activity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	DU145 prostate cancer cells
Concentration:	10, 25, 50, 75, 100 µM
Incubation Time:	24 and 48 hours
Result:	Inhibited DU145 cell proliferation in a time- and dose-dependent manner, with the highest inhibition at the maximum concentration.

Apoptosis Analysis^[2]

Cell Line:	DU145 prostate cancer cells
Concentration:	50 µM
Incubation Time:	24 hours
Result:	Induced reactive oxygen species (ROS) generation in DU145 cells, causing mitochondrial membrane depolarization and apoptosis.

In Vivo

Phenazine-1-carboxylic acid (2 and 3 µg/mL, added through E3 medium, single dose) significantly prevented V. anguillarum infection in zebrafish embryos^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Zebrafish embryos infected with Vibrio anguillarum^[4]
Dosage:	2 µg/mL and 3 µg/mL
Administration:	Added through zebrafish embryo E3 medium, single dose
Result:	Effectively inhibited V. anguillarum infection in zebrafish embryos and increased embryo hatching rate.

Molecular Weight

224.21

Formula

C₁₃H₈N₂O₂

CAS No.

2538-68-3

Appearance

Solid

Color

Light yellow to green yellow

SMILES

O=C(O)C1=C(N=C(C=CC=C2)C2=N3)C3=CC=C1

Structure Classification

Initial Source

Microorganisms

Marine natural products

Truncatella angustata

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (22.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.4601 mL	22.3005 mL	44.6010 mL
5 mM	0.8920 mL	4.4601 mL	8.9202 mL
10 mM	0.4460 mL	2.2301 mL	4.4601 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.88%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (252 KB)

Handling Instructions (2659 KB)

References

[1]. Cimmino A, et al. Phenazine-1-Carboxylic Acid (PCA), Produced for the First Time as an Antifungal Metabolite by Truncatella angustata, a Causal Agent of Grapevine Trunk Diseases (GTDs) in Iran. J Agric Food Chem. 2021 Oct 20;69(41):12143-12147. [Content Brief]

[2]. Karuppiah V, et al. Phenazine-1-carboxylic acid-induced programmed cell death in human prostate cancer cells is mediated by reactive oxygen species generation and mitochondrial-related apoptotic pathway[J]. journal of applied biomedicine, 2016, 14(3): 199-209.

[3]. Denning GM, et al. Phenazine-1-carboxylic acid, a secondary metabolite of Pseudomonas aeruginosa, alters expression of immunomodulatory proteins by human airway epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2003 Sep;285(3):L584-92. [Content Brief]

[4]. Zhang L, et al. Antagonistic activity and mode of action of phenazine-1-carboxylic acid, produced by marine bacterium Pseudomonas aeruginosa PA31x, against Vibrio anguillarum in vitro and in a zebrafish in vivo model[J]. Frontiers in microbiology, 2017, 8: 289. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.4601 mL	22.3005 mL	44.6010 mL	111.5026 mL
	5 mM	0.8920 mL	4.4601 mL	8.9202 mL	22.3005 mL
	10 mM	0.4460 mL	2.2301 mL	4.4601 mL	11.1503 mL
	15 mM	0.2973 mL	1.4867 mL	2.9734 mL	7.4335 mL
	20 mM	0.2230 mL	1.1150 mL	2.2301 mL	5.5751 mL