2. Anti-infection
  3. Antibiotic Fungal Bacterial Parasite
  4. Phenothiazine

Phenothiazine  (Synonyms: Thiodiphenylamine)

Cat. No.: HY-Y0055 Purity: 99.90%
SDS
COA
Handling Instructions Technical Support

Phenothiazine is an antibiotic which has insecticidal, fungicidal, antibacterial and anthelmintic activities. Phenothiazine also can be used for the research of neurological diseases.

For research use only. We do not sell to patients.

Phenothiazine Chemical Structure

Phenothiazine Chemical Structure

CAS No. : 92-84-2

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Free Sample (0.1 - 0.5 mg)   Apply Now  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
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5 g   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Phenothiazine:

Phenothiazine Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Phenothiazine

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  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Phenothiazine is an antibiotic which has insecticidal, fungicidal, antibacterial and anthelmintic activities. Phenothiazine also can be used for the research of neurological diseases[1][2].

Cellular Effect
Cell Line Type Value Description References
HT-1080 EC50
0.049 μM
Compound: phenothiazine
Inhibition of erastin-induced ferroptosis in human HT-1080 cells assessed as cell viability incubated for 48 hrs by MTT assay
Inhibition of erastin-induced ferroptosis in human HT-1080 cells assessed as cell viability incubated for 48 hrs by MTT assay
[PMID: 33065375]
K562 IC50
> 100 μM
Compound: 9
Antiproliferative activity against human K562 cells after 48 hrs by hemocytometric analysis
Antiproliferative activity against human K562 cells after 48 hrs by hemocytometric analysis
[PMID: 21563750]
U-937 IC50
1.39 μM
Compound: 9
Cytotoxicity in UV-irradiated human U937 cells exposed to photosensitizer for 2 hrs before irradiation for 20 mins by WST-1 assay
Cytotoxicity in UV-irradiated human U937 cells exposed to photosensitizer for 2 hrs before irradiation for 20 mins by WST-1 assay
[PMID: 24900465]
In Vitro

Phenothiazine inhibits cell death caused by oxidative stress in cells HT22 (causes by glutamate, EC50=0.02 μM), SK-N-MC (causes by H2O2, EC50=0.02 μM)and SH-SY5Y (causes by MPP+, EC50=0.02 μM). Phenothiazine inhibits lipid peroxidation in Ascorbate (HY-B0166)-damaged pig brain membranes, exhibits antioxidant efficacy[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Phenothiazine Related Antibodies
In Vivo

Phenothiazine causes acute hemolytic anemia, exhibits neurotoxicity and phototoxicity in pigs and horses[2].
Phenothiazine protects dopaminergic neurons from the toxic effects of MPP+ and Rotenone (HY-B1756) in C. elegans models[3].
Phenothiazine (5-30 mg/kg, sc, single dose) reduces brain lesions, and exhibits neuroprotective efficacy in traumatic brain injury (TBI) mouse models[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Traumatic brain injury (TBI) mouse models[4]
Dosage: 5-30 mg/kg
Administration: sc, single dose
Result: Reduced brain lesions and weight loss.
Molecular Weight

199.28

Formula

C12H9NS
CAS No.
Appearance

Solid

Color

Off-white to light green

SMILES

C12=CC=CC=C1NC3=C(C=CC=C3)S2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (501.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.0181 mL 25.0906 mL 50.1812 mL
5 mM 1.0036 mL 5.0181 mL 10.0362 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (12.55 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (12.55 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.90%

SDS (761 KB)

COA (250 KB) LCMS (93 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.0181 mL 25.0906 mL 50.1812 mL 125.4529 mL
5 mM 1.0036 mL 5.0181 mL 10.0362 mL 25.0906 mL
10 mM 0.5018 mL 2.5091 mL 5.0181 mL 12.5453 mL
15 mM 0.3345 mL 1.6727 mL 3.3454 mL 8.3635 mL
20 mM 0.2509 mL 1.2545 mL 2.5091 mL 6.2726 mL
25 mM 0.2007 mL 1.0036 mL 2.0072 mL 5.0181 mL
30 mM 0.1673 mL 0.8364 mL 1.6727 mL 4.1818 mL
40 mM 0.1255 mL 0.6273 mL 1.2545 mL 3.1363 mL
50 mM 0.1004 mL 0.5018 mL 1.0036 mL 2.5091 mL
60 mM 0.0836 mL 0.4182 mL 0.8364 mL 2.0909 mL
80 mM 0.0627 mL 0.3136 mL 0.6273 mL 1.5682 mL
100 mM 0.0502 mL 0.2509 mL 0.5018 mL 1.2545 mL
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Phenothiazine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phenothiazine92-84-2ThiodiphenylamineAntibioticFungalBacterialParasiteinsecticidalfungicidalantibacterialanthelminticneurologicalInhibitorinhibitorinhibit

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