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  6. Phytohemagglutinin

Phytohemagglutinin  (Synonyms: PHA-M)

Cat. No.: HY-N7038 Purity: ≥80.0%
Data Sheet
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Help & FAQs

Phytohemagglutinin (PHA-M), the major seed lectin of the common bean, Phaseolus vulgaris, accumulates in the parenchyma cells of the cotyledons. Phytohemagglutinin is a T-cell activator. Stimulation of human mononuclear leukocytes by Phytohemagglutinin induces the expression of ChAT mRNA, and potentiated ACh synthesis.

For research use only. We do not sell to patients.

Phytohemagglutinin Chemical Structure

Phytohemagglutinin Chemical Structure

CAS No. : 9008-97-3

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Based on 4 publication(s) in Google Scholar

Other Forms of Phytohemagglutinin:

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  • Purity & Documentation

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Description

Phytohemagglutinin (PHA-M), the major seed lectin of the common bean, Phaseolus vulgaris, accumulates in the parenchyma cells of the cotyledons. Phytohemagglutinin is a T-cell activator. Stimulation of human mononuclear leukocytes by Phytohemagglutinin induces the expression of ChAT mRNA, and potentiated ACh synthesis[1].

IC50 & Target

Apoptosis[1]
In Vitro

Phytohemagglutinin (PHA-M) binds to the membranes of T-cells, stimulates metabolic activity, cell division, and involves inflammatory pathways[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis[1]

Cell Line: A20 (mouse B cell lymphoma, TLB-208), BW5147.3 (mouse T cell lymphoma, TIB-47), EL4 (mouse T cell lymphoma, TIB-39)[1]
Concentration: 20 μg/ml
Incubation Time: 20-48 hours
Result: Enhanced apoptosis in A20, BW5147.3 and EL4 cells.
In Vivo

Phytohemagglutinin (100 μg; i.p.; daily for 2 days) inhibits A20 tumor growth[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: CB17-SCID Mice (bearing A20 cells)[1]
Dosage: 100 μg
Administration: I.p.; daily for 2 days
Result: Inhibited A20 tumor growth.
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

[Phytohemagglutinin]
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

H2O : 10 mg/mL (Need ultrasonic)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL; Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
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Purity & Documentation

Purity: ≥80.0%

Data Sheet (270 KB) SDS (251 KB)

COA (241 KB)

Handling Instructions (2659 KB)
References
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Phytohemagglutinin Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

Keywords:

Phytohemagglutinin9008-97-3PHA-MOthersT-cellB-celldivisioncancerinflammatorylymphoidtumorInhibitorinhibitorinhibit

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