1-EBIO (Synonyms: 1-Ethyl-2-benzimidazolinone)

Name: 1-EBIO
Brand: MedChemExpress
SKU: HY-101360
Rating: 4.5 (1 reviews)

Cat. No.: HY-101360 Purity: 99.96%: Data Sheet
SDS

COA
Handling Instructions
FAQs
Technical Support

Solubility

In Vivo Dissolution Calculator

Help & FAQs

1-EBIO is an activator of Ca²⁺ sensitive K⁺ channels. 1-EBIO is used to study the role of K⁺ channels in diverse physiological functions.

For research use only. We do not sell to patients.

1-EBIO Chemical Structure

CAS No. : 10045-45-1

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.2 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 31	In-stock	Estimated Time of Arrival: June 3
Solution
10 mM * 1 mL in DMSO	USD 33	In-stock	Estimated Time of Arrival: June 3
Solid
25 mg	USD 30	In-stock	Estimated Time of Arrival: June 3
50 mg	USD 50	In-stock	Estimated Time of Arrival: June 3
100 mg	USD 70	In-stock	Estimated Time of Arrival: June 3
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

1-EBIO is an activator of Ca²⁺ sensitive K⁺ channels. 1-EBIO is used to study the role of K⁺ channels in diverse physiological functions^[1].

Molecular Weight

162.19

Formula

C₉H₁₀N₂O

CAS No.

10045-45-1

Appearance

Solid

Color

Light yellow to light brown

SMILES

O=C1NC2=CC=CC=C2N1CC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (616.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.1656 mL	30.8280 mL	61.6561 mL
5 mM	1.2331 mL	6.1656 mL	12.3312 mL
10 mM	0.6166 mL	3.0828 mL	6.1656 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (15.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (15.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (15.41 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, PEG300/PEG400, Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Purity & Documentation

Purity: 99.96%

Select Batch:

Data Sheet (273 KB) SDS (392 KB)

COA (251 KB) HNMR (245 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Devor DC, et al. Modulation of Cl- secretion by benzimidazolones. I. Direct activation of a Ca(2+)-dependent K+ channel. Am J Physiol. 1996 Nov;271(5 Pt 1):L775-84. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	6.1656 mL	30.8280 mL	61.6561 mL	154.1402 mL
	5 mM	1.2331 mL	6.1656 mL	12.3312 mL	30.8280 mL
	10 mM	0.6166 mL	3.0828 mL	6.1656 mL	15.4140 mL
	15 mM	0.4110 mL	2.0552 mL	4.1104 mL	10.2760 mL
	20 mM	0.3083 mL	1.5414 mL	3.0828 mL	7.7070 mL
	25 mM	0.2466 mL	1.2331 mL	2.4662 mL	6.1656 mL
	30 mM	0.2055 mL	1.0276 mL	2.0552 mL	5.1380 mL
	40 mM	0.1541 mL	0.7707 mL	1.5414 mL	3.8535 mL
	50 mM	0.1233 mL	0.6166 mL	1.2331 mL	3.0828 mL
	60 mM	0.1028 mL	0.5138 mL	1.0276 mL	2.5690 mL
	80 mM	0.0771 mL	0.3854 mL	0.7707 mL	1.9268 mL
	100 mM	0.0617 mL	0.3083 mL	0.6166 mL	1.5414 mL

1-EBIO Related Classifications

Neurological Disease

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?
1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.
How should solution-form recombinant proteins be stored?
1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that
Why is it necessary to add carrier proteins?
Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.
Carrier protein types and options?
In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

Keywords:

1-EBIO10045-45-11-Ethyl-2-benzimidazolinonePotassium ChannelKcsAInhibitorinhibitorinhibit

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1-EBIO (Synonyms: 1-Ethyl-2-benzimidazolinone)

Customer Review

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•Related Screening Libraries:

Biological Activity

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References

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Molarity Calculator

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Complete Stock Solution Preparation Table

1-EBIO Related Classifications

Keywords:

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