PluriSIn #2 

PluriSIn #2 is a selective transcriptional inhibitor of topoisomerase II α (TOP2A). PluriSIn #2 is a compound that selectively eliminates undifferentiated human pluripotent stem cells (hPSCs)^[1].

PluriSIn #2 (20 µM; 12 hours) treatment reduces TOP2A expression at the protein level^[1].
A 24h exposure of PluriSIn #2 (20 µM) is sufficient to induce massive cell death in hPSCs, but doesn’t affect the viability of various other cell types derived from them^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

249.20

C₁₁H₈FN₃O₃

56563-17-8

Solid

White to off-white

O=C(N(C(N1)=O)C=C(F)C1=O)NC2=CC=CC=C2

Room temperature in continental US; may vary elsewhere.

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Uri Ben-David, et al. Brief reports: Controlling the survival of human pluripotent stem cells by small molecule-based targeting of topoisomerase II alpha. Stem Cells. 2015 Mar;33(3):1013-9.  [Content Brief]