(±)-Evodiamine

Name: (±)-Evodiamine
Brand: MedChemExpress
SKU: HY-N0114A
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0114A Purity: 99.74%: FAQs
Data Sheet SDS COA Handling Instructions

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(±)-Evodiamine, a quinazolinocarboline alkaloid, is a Top1 inhibitor. Evodiamine exhibits anti-inflammatory, antiobesity, and antitumor effects. (±)-Evodiamine inhibits the proliferation of a wide variety of tumor cells by inducing their apoptosis.

For research use only. We do not sell to patients.

(±)-Evodiamine Chemical Structure

CAS No. : 518-18-3

Size	Price	Stock	Quantity
250 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 80	In-stock	Estimated Time of Arrival: December 31
1 g	USD 120	In-stock	Estimated Time of Arrival: December 31
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10 g		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of (±)-Evodiamine:

Evodiamine In-stock

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Topoisomerase Topo I Topo II

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Top1

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	0.98 μM Compound: 1	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 27178387]
A549	IC₅₀	77.24 μM Compound: 3; Evo	Antiproliferative activity against human A549 cells assessed as inhibition rate incubated for 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as inhibition rate incubated for 72 hrs by MTT assay	[PMID: 34844412]
Bel-7402	IC₅₀	1.19 μM Compound: 1	Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 29635169]
Bel-7402	IC₅₀	1.23 μM Compound: 1	Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay Antiproliferative activity against human Bel7402 cells after 72 hrs by MTT assay	[PMID: 27178387]
BGC-823	IC₅₀	1.16 μM Compound: 1	Antiproliferative activity against human BGC823 cells after 72 hrs by MTT assay Antiproliferative activity against human BGC823 cells after 72 hrs by MTT assay	[PMID: 27178387]
Caco-2	IC₅₀	2.73 μM Compound: 1	Antiproliferative activity against human Caco2 cells after 72 hrs by MTT assay Antiproliferative activity against human Caco2 cells after 72 hrs by MTT assay	[PMID: 29635169]
CHO	EC₅₀	0.86 μM Compound: 1	Agonist activity at rat TRPV1 expressed in CHO cells assessed as induction of 45Ca2+ uptake measured after 5 mins by scintillation counting method Agonist activity at rat TRPV1 expressed in CHO cells assessed as induction of 45Ca2+ uptake measured after 5 mins by scintillation counting method	[PMID: 27159637]
HCCLM9	IC₅₀	9.27 μM Compound: EVO	Antiproliferative activity against human HCCLM9 cells treated for 72 hrs by MTT assay Antiproliferative activity against human HCCLM9 cells treated for 72 hrs by MTT assay	[PMID: 35639640]
HCT-116	IC₅₀	32.67 μM Compound: 3; Evo	Antiproliferative activity against human HCT-116 cells assessed as inhibition rate incubated for 72 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as inhibition rate incubated for 72 hrs by MTT assay	[PMID: 34844412]
HEK293	IC₅₀	> 100 μM Compound: 3; Evo	Antiproliferative activity against HEK293 cells assessed as inhibition rate incubated for 72 hrs by MTT assay Antiproliferative activity against HEK293 cells assessed as inhibition rate incubated for 72 hrs by MTT assay	[PMID: 34844412]
HEK293	EC₅₀	0.1 μM Compound: 1	Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 0.1 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM) Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 0.1 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM)	[PMID: 27159637]
HEK293	EC₅₀	0.18 μM Compound: 1	Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 0.6 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM) Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 0.6 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM)	[PMID: 27159637]
HEK293	EC₅₀	0.33 μM Compound: 1	Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 1 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM) Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 1 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM)	[PMID: 27159637]
HEK293	IC₅₀	0.44 μM Compound: 1	Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as inhibition of capsaicin-induced inward current at -60 mV holding potential pretreated for 180 secs prior to capsaicin addition in presence of extracellular calcium by whole-cel Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as inhibition of capsaicin-induced inward current at -60 mV holding potential pretreated for 180 secs prior to capsaicin addition in presence of extracellular calcium by whole-cel	[PMID: 27159637]
HEK293	EC₅₀	0.6 μM Compound: 1	Agonist activity at rat TRPV1 channel expressed in HEK293 cells assessed as induction of channel current at -60 mV holding potential by whole-cell patch-clamp method Agonist activity at rat TRPV1 channel expressed in HEK293 cells assessed as induction of channel current at -60 mV holding potential by whole-cell patch-clamp method	[PMID: 27159637]
HEK293	EC₅₀	2.38 μM Compound: 1	Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 10 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM) Partial antagonist activity at rat TRPV1 expressed in HEK293 cells assessed as capsaicin EC50 for induction of channel current at 10 uM at -60 mV holding potential by whole-cell patch clamp method (Rvb = 0.06 uM)	[PMID: 27159637]
HepG2	IC₅₀	2.67 μM Compound: EVO	Antiproliferative activity against human HepG2 cells treated for 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells treated for 72 hrs by MTT assay	[PMID: 35639640]
HepG2	IC₅₀	9.32 μM Compound: 1	Antiproliferative activity against human HepG2 cells treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
HL-60	IC₅₀	1.36 μM Compound: 1	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay	[PMID: 29635169]
HL-60	IC₅₀	1.39 μM Compound: 1	Antiproliferative activity against human HL60 cells treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human HL60 cells treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
Huh-7	IC₅₀	2.18 μM Compound: EVO	Antiproliferative activity against human Huh-7 cells treated for 72 hrs by MTT assay Antiproliferative activity against human Huh-7 cells treated for 72 hrs by MTT assay	[PMID: 35639640]
L02	IC₅₀	0.34 μM Compound: 1	Antiproliferative activity against human L02 cells after 72 hrs by MTT assay Antiproliferative activity against human L02 cells after 72 hrs by MTT assay	[PMID: 27178387]
L02	IC₅₀	3.95 μM Compound: EVO	Antiproliferative activity against human L02 cells treated for 72 hrs by MTT assay Antiproliferative activity against human L02 cells treated for 72 hrs by MTT assay	[PMID: 35639640]
MCF-10A	IC₅₀	> 100 μM Compound: 3; Evo	Antiproliferative activity against human MCF-10A cells assessed as inhibition rate incubated for 72 hrs by MTT assay Antiproliferative activity against human MCF-10A cells assessed as inhibition rate incubated for 72 hrs by MTT assay	[PMID: 34844412]
MCF7	IC₅₀	28.53 μM Compound: 1	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 29635169]
MDA-MB-231	IC₅₀	13.3 μM Compound: 3; Evo	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition rate incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition rate incubated for 72 hrs by MTT assay	[PMID: 34844412]
MDA-MB-435	IC₅₀	6.3 μM Compound: 3; Evo	Antiproliferative activity against human MDA-MB-435 cells assessed as inhibition rate incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-435 cells assessed as inhibition rate incubated for 72 hrs by MTT assay	[PMID: 34844412]
PBMC	IC₅₀	20.96 μM Compound: 1	Antiproliferative activity against human PBMC cells after 72 hrs by MTT assay Antiproliferative activity against human PBMC cells after 72 hrs by MTT assay	[PMID: 29635169]
PBMC	IC₅₀	22.87 μM Compound: 1	Antiproliferative activity against human PBMC treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human PBMC treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
PC-3	IC₅₀	13.97 μM Compound: 1	Antiproliferative activity against human PC3 cells treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human PC3 cells treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]
SGC-7901	IC₅₀	6.93 μM Compound: 1	Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay Antiproliferative activity against human SGC7901 cells after 72 hrs by MTT assay	[PMID: 29635169]
SK-HEP1	IC₅₀	2.92 μM Compound: EVO	Antiproliferative activity against human SK-HEP1 cells treated for 72 hrs by MTT assay Antiproliferative activity against human SK-HEP1 cells treated for 72 hrs by MTT assay	[PMID: 35639640]
SMMC-7721	IC₅₀	3.04 μM Compound: EVO	Antiproliferative activity against human SMMC-7721 cells treated for 72 hrs by MTT assay Antiproliferative activity against human SMMC-7721 cells treated for 72 hrs by MTT assay	[PMID: 35639640]
THP-1	IC₅₀	3.37 μM Compound: 1	Antiproliferative activity against human THP1 cells treated for 24 hrs measured after 72 hrs by MTT assay Antiproliferative activity against human THP1 cells treated for 24 hrs measured after 72 hrs by MTT assay	[PMID: 29037951]

Molecular Weight

303.36

Formula

C₁₉H₁₇N₃O

CAS No.

518-18-3

Appearance

Solid

Color

Off-white to light brown

SMILES

O=C1C2=CC=CC=C2N(C)C(N1CC3)C4=C3C5=CC=CC=C5N4

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 10 mg/mL (32.96 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2964 mL	16.4821 mL	32.9641 mL
5 mM	0.6593 mL	3.2964 mL	6.5928 mL
10 mM	0.3296 mL	1.6482 mL	3.2964 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 1 mg/mL (3.30 mM); Clear solution

This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.74%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (254 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Dong G, et, al. New tricks for an old natural product: discovery of highly potent evodiamine derivatives as novel antitumor agents by systemic structure-activity relationship analysis and biological evaluations. J Med Chem. 2012 Sep 13;55(17):7593-613. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2964 mL	16.4821 mL	32.9641 mL	82.4103 mL
	5 mM	0.6593 mL	3.2964 mL	6.5928 mL	16.4821 mL
	10 mM	0.3296 mL	1.6482 mL	3.2964 mL	8.2410 mL
	15 mM	0.2198 mL	1.0988 mL	2.1976 mL	5.4940 mL
	20 mM	0.1648 mL	0.8241 mL	1.6482 mL	4.1205 mL
	25 mM	0.1319 mL	0.6593 mL	1.3186 mL	3.2964 mL
	30 mM	0.1099 mL	0.5494 mL	1.0988 mL	2.7470 mL

(±)-Evodiamine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(±)-Evodiamine518-18-3TopoisomeraseApoptosisquinazolinocarbolinealkaloidTop1anti-inflammatoryantiobesityantitumorapoptosisInhibitorinhibitorinhibit

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(±)-Evodiamine

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View All Topoisomerase Isoform Specific Products:

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References

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Complete Stock Solution Preparation Table

(±)-Evodiamine Related Classifications

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