APOBEC3A Protein, Human (His-SUMO)

APOBEC3A protein is a DNA deaminase that limits the mobility of viruses, foreign DNA, and retrotransposons. APOBEC3A Protein, Human (His-SUMO) is the recombinant human-derived APOBEC3A protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.

APOBEC3A protein functions as a DNA deaminase, specifically a cytidine deaminase, exerting restriction activity against viruses, foreign DNA, and the mobility of retrotransposons. It demonstrates antiviral efficacy against adeno-associated virus (AAV) and human T-cell leukemia virus type 1 (HTLV-1), potentially impeding the mobility of both LTR and non-LTR retrotransposons. APOBEC3A selectively targets single-stranded DNA, exhibiting the capacity to deaminate both methylcytosine and cytosine in foreign DNA. Additionally, it can induce somatic hypermutation in both nuclear and mitochondrial DNA, suggesting its involvement in genomic diversity. Furthermore, APOBEC3A may contribute to the epigenetic regulation of gene expression through the active process of DNA demethylation, highlighting its multifaceted role in cellular defense mechanisms and potential impact on the epigenetic landscape.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His;N-SUMO

P31941-1 (M1-N199)

100913187  [NCBI]

MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTSVKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSWGCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWDTFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN

Approximately 39 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.