1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2) Serine/Threonine Kinase Proteins
  4. Glycogen Synthase Kinase-3 (GSK-3)
  5. GSK-3 beta
  6. GSK-3 beta Protein, Mouse (sf9, His)

GSK-3 beta Protein, Mouse (sf9, His)

Cat. No.: HY-P73090
COA Handling Instructions

GSK-3 beta is a protein kinase that regulates cellular circadian rhythm, autophagy, and apoptosis. GSK-3 beta Protein, Mouse (sf9, His) is the recombinant mouse-derived GSK-3 beta protein, expressed by Sf9 insect cells , with N-10*His labeled tag. The total length of GSK-3 beta Protein, Mouse (sf9, His) is 420 a.a., with molecular weight of ~47 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
5 μg $93 In-stock
10 μg $148 In-stock
20 μg $237 In-stock
50 μg $450 In-stock
100 μg   Get quote  

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Description

GSK-3 beta is a protein kinase that regulates cellular circadian rhythm, autophagy, and apoptosis. GSK-3 beta Protein, Mouse (sf9, His) is the recombinant mouse-derived GSK-3 beta protein, expressed by Sf9 insect cells , with N-10*His labeled tag. The total length of GSK-3 beta Protein, Mouse (sf9, His) is 420 a.a., with molecular weight of ~47 kDa.

Background

GSK-3 beta protein, a constitutively active kinase, serves as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling, and the regulation of transcription factors and microtubules. It achieves this by phosphorylating and inactivating key substrates such as glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU, and MACF1. Primed phosphorylation is a prerequisite for the majority of its substrates. In skeletal muscle, GSK-3 beta contributes to insulin regulation of glycogen synthesis by inhibiting GYS1 activity. It may also mediate insulin resistance by regulating activation of transcription factors. Additionally, GSK-3 beta plays a role in protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5). In Wnt signaling, it forms a multimeric complex with APC, AXIN1, and CTNNB1/beta-catenin, phosphorylating CTNNB1 and targeting it for degradation via ubiquitin/proteasomes. GSK-3 beta is involved in various cellular processes, including regulating replication in pancreatic beta-cells, influencing apoptosis, participating in ERBB2-dependent stabilization of microtubules, and controlling cell polarity and axon outgrowth. It also plays a role in the circadian clock regulation, autophagy, and the negative regulation of the extrinsic apoptotic signaling pathway. Moreover, GSK-3 beta phosphorylates several proteins, including E2F1, FXR1, and interleukin-22 receptor subunit IL22RA1, affecting their stability and function.

Biological Activity

1.The specific activity was determined to be > 20 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.
2. Immobilized GSK-3 beta Protein, Mouse (sf9, His) at 10 μg/mL (100 μl/well) can bind human HG3C-CTNNB1 and the EC50 is 0.15-0.35 μg/mL.

Species

Mouse

Source

Sf9 insect cells

Tag

N-10*His

Accession

Q9WV60 (M1-T420)

Gene ID
Molecular Construction
N-term
10*His
GSK-3 beta (M1-T420)
Accession # Q9WV60
C-term
Synonyms
Glycogen synthase kinase-3 beta; GSK-3 beta; Gsk3b
AA Sequence

MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASPPANATAASDTNAGDRGQTNNAASASASNST

Molecular Weight

Approximately 47 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 25% glycerol, 0.2 mM DTT, pH 7.4

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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GSK-3 beta Protein, Mouse (sf9, His)
Cat. No.:
HY-P73090
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