2. Recombinant Proteins
  3. Viral Proteins Biotinylated Proteins
  4. Influenza Viruses Proteins
  5. Influenza Hemagglutinin
  6. HA/Hemagglutinin Protein, H1N1 (Biotinylated, ACP41953.1, HEK293, His)

HA/Hemagglutinin Protein, H1N1 (Biotinylated, ACP41953.1, HEK293, His)

Cat. No.: HY-P74038
Data Sheet
SDS
COA
Handling Instructions Technical Support

HA/Hemagglutinin Protein is a viral surface glycoprotein that mediates virus adsorption to host cells and promotes membrane fusion required for infection. HA/Hemagglutinin Protein plays a key role in the process of viral infection of host cells. HA/Hemagglutinin Protein, H1N1 (Biotinylated, ACP41953.1, HEK293, His) is a recombinant HA/Hemagglutinin protein expressed by HEK293 and carrying a C-His tag.

For research use only. We do not sell to patients.

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20 μg USD 326 In-stock

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Description

HA/Hemagglutinin Protein is a viral surface glycoprotein that mediates virus adsorption to host cells and promotes membrane fusion required for infection. HA/Hemagglutinin Protein plays a key role in the process of viral infection of host cells. HA/Hemagglutinin Protein, H1N1 (Biotinylated, ACP41953.1, HEK293, His) is a recombinant HA/Hemagglutinin protein expressed by HEK293 and carrying a C-His tag[1][2].
Background

HA/Hemagglutinin is a glycoprotein on the surface of viruses such as influenza viruses and one of the major antigenic components. The structure of HA/Hemagglutinin is divided into four subdomains: receptor-binding, vestigial esterase, fusion, and membrane anchor. The core functions of HA/Hemagglutinin are mainly to recognize host cell receptors, promote virus-cell membrane fusion, and trigger immune responses. HA/Hemagglutinin initiates infection by binding to sialic acid receptors on the cell surface and mediates membrane fusion after cleavage by host proteases[1][2].
In Vitro

HA/Hemagglutinin Protein (H1N1; 0-10 μg/mL; 6 h) can promote the secretion of TNF-α and IL-12 p70, as well as the expression of MHC-II, CD40 and CD86 in mouse bone marrow-derived myeloid dendritic cells[3].
In Vivo

HA/Hemagglutinin Protein (H1N1; 10-50 μg; intramuscular injection; twice) induces the production of neutralizing antibodies in BALB/c mice and New Zealand white rabbits, and the sera exhibits hemagglutination-inhibiting activity[4].
Species

Virus
Source

HEK293
Tag

C-His
Accession

ACP41953.1 (D18-Q529)
Gene ID

23308115  [NCBI]
Molecular Construction
N-term
H1N1 HA (D18-Q529)
Accession # ACP41953.1
His
C-term
Synonyms
HA; Hemagglutinin; HA/Hemagglutinin Protein, H1N1 (A/California/07/2009, HEK293, His)
Molecular Weight

Approximately 59 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder
Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 15 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping

Room temperature in continental US; may vary elsewhere.
Documentation
Data Sheet (262 KB) SDS (251 KB)

COA (205 KB)

Handling Instructions (2659 KB)
References

HA/Hemagglutinin Protein, H1N1 (Biotinylated, ACP41953.1, HEK293, His) Related Classifications

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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