IIdD Protein, E.coli (His)

GLO1/Glyoxalase I Protein catalyzes the conversion of hemimercaptal to S-lactoylglutathione, detoxifying cytotoxic methylglyoxal produced in glycolysis. Beyond detoxification, GLO1 regulates NF-kappa-B transcriptional activity in TNF-induced pathways, indicating its role in inflammation. Additionally, GLO1 is crucial for normal osteoclastogenesis, emphasizing its broader involvement in cellular processes. IIdD Protein, E.coli (His) is the recombinant E. coli-derived IIdD protein, expressed by E. coli , with N-His labeled tag.

GLO1/Glyoxalase I protein serves as a catalyst in the conversion of hemimercaptal, generated from the reaction between methylglyoxal and glutathione, into S-lactoylglutathione. This enzymatic activity plays a crucial role in the detoxification of methylglyoxal, a cytotoxic byproduct of glycolysis. Additionally, GLO1 is implicated in the regulation of TNF-induced transcriptional activity of NF-kappa-B, suggesting its involvement in inflammatory signaling pathways. Furthermore, GLO1 is essential for normal osteoclastogenesis, highlighting its significance in cellular processes beyond its role in detoxification.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

N-His

A8A670 (M1-A396)

/

MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSEVALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIPFTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPTPGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLEDYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLYALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAKGNAA

Approximately 44 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.