IIdD Protein, E.coli (His)

GLO1/Glyoxalase I Protein catalyzes the conversion of hemimercaptal to S-lactoylglutathione, detoxifying cytotoxic methylglyoxal produced in glycolysis. Beyond detoxification, GLO1 regulates NF-kappa-B transcriptional activity in TNF-induced pathways, indicating its role in inflammation. Additionally, GLO1 is crucial for normal osteoclastogenesis, emphasizing its broader involvement in cellular processes. IIdD Protein, E.coli (His) is the recombinant E. coli-derived IIdD protein, expressed by E. coli , with N-His labeled tag. The total length of IIdD Protein, E.coli (His) is 396 a.a., with molecular weight of ~46.7 kDa.

GLO1/Glyoxalase I protein serves as a catalyst in the conversion of hemimercaptal, generated from the reaction between methylglyoxal and glutathione, into S-lactoylglutathione. This enzymatic activity plays a crucial role in the detoxification of methylglyoxal, a cytotoxic byproduct of glycolysis. Additionally, GLO1 is implicated in the regulation of TNF-induced transcriptional activity of NF-kappa-B, suggesting its involvement in inflammatory signaling pathways. Furthermore, GLO1 is essential for normal osteoclastogenesis, highlighting its significance in cellular processes beyond its role in detoxification.

E.coli

E. coli

N-His

A8A670 (1M-396A)

57729941

MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSEVALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIPFTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPTPGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLEDYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHGGRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLYALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAKGNAA

Approximately 46.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.