Insulysin/IDE Protein, Human (HEK293, His)

Insulin/IDE protein is the key to the decomposition of cellular peptides. It participates in the degradation of insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kinins and other peptides, and affects intercellular peptide signaling. Substrate binding induces conformational changes that promote degradation of larger substrates such as insulin. Insulysin/IDE Protein, Human (HEK293, His) is the recombinant human-derived Insulysin/IDE protein, expressed by HEK293 , with C-6*His labeled tag.

Insulysin/IDE protein plays a crucial role in the intricate cellular breakdown of various peptides, including insulin, APP peptides, IAPP peptides, natriuretic peptides, glucagon, bradykinin, kallidin, and others. This multifaceted involvement underscores its significance in intercellular peptide signaling and the regulation of peptide hormone cascades. Substrate binding induces vital conformational changes, enabling the degradation of larger substrates, such as insulin. Insulysin/IDE contributes to the intricate regulation of blood glucose homeostasis by participating in the degradation of insulin, glucagon, and IAPP. Additionally, it plays a pivotal role in the clearance of amyloidogenic peptides derived from APP, impacting neuronal and microglial processes. The protein is also implicated in the degradation of natriuretic peptides, ANP, BNP, and CNP, thereby inactivating their ability to raise intracellular cGMP. Furthermore, Insulysin/IDE is involved in antigen processing, producing antigenic peptides presented to cytotoxic T lymphocytes. In microbial infection, the membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P14735-1 (M42-L1019)

3416  [NCBI]

MNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Approximately 120.0 kDa under reducing (R) condition.

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 10% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.