2. MAPK/ERK Pathway
  3. Ribosomal S6 Kinase (RSK)
  4. RSK4-IN-1 TFA

RSK4-IN-1 TFA is a potent RSK4 inhibitor with a IC50 value of 9.5 nM. RSK4-IN-1 TFA has antitumor activity.

For research use only. We do not sell to patients.

RSK4-IN-1 TFA Chemical Structure

RSK4-IN-1 TFA Chemical Structure

Size Price Stock Quantity
5 mg USD 310 In-stock
10 mg USD 500 In-stock
25 mg USD 980 In-stock
50 mg USD 1600 In-stock
100 mg USD 2600 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of RSK4-IN-1 TFA:

RSK4-IN-1 TFA Related Antibodies

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p70S6K RSK1 RSK2 RSK3 RSK4

  • Biological Activity

  • Purity & Documentation

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Description

RSK4-IN-1 TFA is a potent RSK4 inhibitor with a IC50 value of 9.5 nM. RSK4-IN-1 TFA has antitumor activity[1].

IC50 & Target

RSK4

9.5 nM (IC50)

Molecular Weight

504.41

Formula

C21H21F5N4O5
Appearance

Solid

SMILES

CC(C)(C1=CN=C(N=C1N2C3CCCC3)NC4=CC(F)=C(C(F)=C4)O)OC2=O.O=C(O)C(F)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (198.25 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.9825 mL 9.9126 mL 19.8251 mL
5 mM 0.3965 mL 1.9825 mL 3.9650 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: 2.5 mg/mL (4.96 mM); Clear solution; Need ultrasonic

    This protocol yields a clear solution of 2.5 mg/mL.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.9825 mL 9.9126 mL 19.8251 mL 49.5629 mL
5 mM 0.3965 mL 1.9825 mL 3.9650 mL 9.9126 mL
10 mM 0.1983 mL 0.9913 mL 1.9825 mL 4.9563 mL
15 mM 0.1322 mL 0.6608 mL 1.3217 mL 3.3042 mL
20 mM 0.0991 mL 0.4956 mL 0.9913 mL 2.4781 mL
25 mM 0.0793 mL 0.3965 mL 0.7930 mL 1.9825 mL
30 mM 0.0661 mL 0.3304 mL 0.6608 mL 1.6521 mL
40 mM 0.0496 mL 0.2478 mL 0.4956 mL 1.2391 mL
50 mM 0.0397 mL 0.1983 mL 0.3965 mL 0.9913 mL
60 mM 0.0330 mL 0.1652 mL 0.3304 mL 0.8260 mL
80 mM 0.0248 mL 0.1239 mL 0.2478 mL 0.6195 mL
100 mM 0.0198 mL 0.0991 mL 0.1983 mL 0.4956 mL
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RSK4-IN-1 TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RSK4-IN-1Ribosomal S6 Kinase (RSK)S6KRSK4InhibitorESCCAntitumorinhibitorinhibit

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